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A genotype determination method of fad2 gene of Brassica napus

A technology of Brassica napus and determination method, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve problems such as cumbersome work

Inactive Publication Date: 2018-11-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to find the difference in the gene sequence of different materials, it is necessary to carry out gene amplification and sequencing with different templates, which is cumbersome.

Method used

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  • A genotype determination method of fad2 gene of Brassica napus
  • A genotype determination method of fad2 gene of Brassica napus
  • A genotype determination method of fad2 gene of Brassica napus

Examples

Experimental program
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Effect test

Embodiment 1

[0056] (1) Take the seeds of the Brassica napus maintainer line 100B, undergo spaceflight mutagenesis + staggered sodium compound mutagenesis, and after multiple generations of planting, the offspring with stable inheritance of oleic acid properties, that is, the high oleic acid material fad2 of the present invention Gene clones 18, 29, 31, 43, 49 have an oleic acid content of 62.60%; the experiment can also be performed using naturally occurring rapeseed materials with high oleic acid content. High oleic acid content is generally > 60%, expressed in G.

[0057] Take the seeds of the Brassica napus maintainer line 100B harvested without any treatment and grown in the natural state in the field, that is, the clones 6, 13, 19, 53, and 55 of the fad2 gene of the control material of the high-oleic acid material described in the present invention, The content of oleic acid is 56.57%; the reference material is represented by CK.

[0058] (2) According to Peng Qi’s 2011 doctoral dis...

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PUM

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Abstract

The invention provides a classification method, which can be used for quickly and conveniently classifying a genotype of a fad2 gene of cabbage type rape with oleic acid content which is more than 30 percent, and particularly discloses a method for classifying the genotype of the fad2 gene of the cabbage type rape by judging whether basic groups of nucleotides from a forty-first position to a fiftieth position of the fad2 gene of the cabbage type rape are AAAAGTCTGA or not and whether basic groups of nucleotides from a two hundred and thirty-first position to a two hundred and forty-fifth position are TCCCTCACCCTCTCT or not.

Description

technical field [0001] The invention relates to the field of oil crops, in particular to a method for testing and judging that the fad2 gene of Brassica napus belongs to fad2-I type or fad2-II type. Background technique [0002] Brassica napus is an important type of the three major rapeseed resources, and it is also a typical oil crop with a wide planting area in southern my country. Its fatty acid composition is divided into saturated fatty acids and unsaturated fatty acids. Unsaturated fatty acids are beneficial to human health and plant resistance Reverse and post-injury repair play an important role. Fad2 gene encodes oleic acid dehydrogenase, which is a key enzyme for plants to produce polyunsaturated fatty acids and an important enzyme for regulating oleic acid content in Brassica napus. At present, in double-low rape varieties, oleic acid is the main fatty acid with the highest content, followed by linoleic acid and linolenic acid. The oleic acid content of rapeseed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6869
CPCC12Q1/6869C12Q1/6895
Inventor 李壮余世聪蔺丽丽牛应泽吴永成郭世星侯凯
Owner SICHUAN AGRI UNIV
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