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Identification or prediction method of pork quality

A prediction method and meat quality technology, applied in the field of breeding, can solve problems such as differences in pig breeds

Inactive Publication Date: 2013-06-26
SICHUAN CHUANJIAO ECOLOGY PIG IND
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Problems solved by technology

[0009] GYS-1 encodes the rate-limiting enzyme of skeletal muscle glycogen synthesis—glycogen synthase 1, which is a candidate gene that affects meat quality through muscle. It was found through the study of 5 Chinese local breeds and 3 western pig breeds There is a restriction fragment polymorphism in an intron of GYS1, which causes the difference between different pig breeds

Method used

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  • Identification or prediction method of pork quality
  • Identification or prediction method of pork quality
  • Identification or prediction method of pork quality

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Embodiment Construction

[0081] Attached below figure 1 and 2 Introduce the specific embodiment of the present invention in detail, carry out qRT-PCR quantitative analysis on the glycogen synthesis gene and glycolysis regulation gene in the eye muscle of slaughtered pigs, and select the key factor that plays a decisive role in the final meat quality according to the analysis results. function-related genes.

[0082] The root uses glycogen synthesis genes and glycolysis regulatory genes as independent variables, and the final pH (24h) as the dependent variable to conduct simple multiple regression analysis to obtain the relationship between the expression of these genes and the meat quality index (final pH). The specific steps are as shown in the figure below, and finally the relationship between glycogenin-1-like, glycogen synthase-1, PRKAG3, hexokinase-1 and hexokinase-2 and the final pH value is obtained:

[0083] y=5.823-1789.991×x 1 +14.826×x 2 +29.259×x 3 -31.185×x 4 +21.458×x 5 (R=0.947)...

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Abstract

The invention discloses an identification or prediction method of pork quality. The pork quality is identified and predicted by determining expression quantity of gene glycogenin-1-like and gene PRKAG3, the expression quantity of the gene glycogen synthase-1, the expression quantity of the gene hexokinase-1, and the expression quantity of the gene hexokinase-2 through real-time fluorescence quantification PCR (qRT-PCR). The method can be used for judging pork quality performance by detecting the expression quantity of the plurality of key genes, the process that the pig needs to be butchered to determine the pork quality is avoided, and the cost of pork quality performance detection is reduced. More reliable data can be added in the work of pig breeding through the formula, and the breeding accuracy is improved; and meanwhile partial compatriot determining is replaced, and the breeding cost of the enterprise is reduced.

Description

Technical field: [0001] The invention relates to a method for identifying and predicting pork meat quality, and belongs to the field of breeding. Background technique: [0002] Pork is the main source of meat, and pig farming has always been one of the most important components of animal husbandry. With the explosive growth of the world's population and the improvement of people's living standards since modern times, the pig industry, which provides the most important source of meat products for human beings, is also developing rapidly. For a long time in the past, the focus of pig genetics and breeding was mainly on producing the most lean meat in the shortest time with the least amount of feed. After satisfying the demand of "quantity", people began to pay more and more attention to the improvement of "quality". In the 1990s meat quality became the focus of people's breeding work, and with the passage of time this concern has become a focus of more and more investment. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 李雪梅李疏仲李学杰李聪睿张顺华李一汉郑莉
Owner SICHUAN CHUANJIAO ECOLOGY PIG IND
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