SNP molecular marker related to cockscomb developmental character and detection method thereof

A molecular marker and detection method technology, applied in the field of poultry genetic engineering, to achieve the effect of accelerating genetic progress, great economic value and scientific research value, and saving production costs

Active Publication Date: 2018-11-23
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the genetic variation of the CHADL gene and the traits of comb development

Method used

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  • SNP molecular marker related to cockscomb developmental character and detection method thereof
  • SNP molecular marker related to cockscomb developmental character and detection method thereof
  • SNP molecular marker related to cockscomb developmental character and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] The method for detecting the SNP molecular marker comprises the following steps:

[0029] (1) Test material

[0030] The population to be tested comes from Jiangsu Lihua Animal Husbandry Co., Ltd., the terminal male parent A line roosters used for breeding of new processing-type high-quality broiler breeds (matching lines). This strain has the characteristics of green feet, jute feathers, and well-developed combs. All families were mixed in groups, kept in cages, fed freely, and had adequate drinking water. Feed to 20 weeks of age and reach sexual maturity, take blood samples, measure the comb area and height of each chicken and record the data.

[0031] (2) Extraction of genomic DNA

[0032] After the individual chicken was weighed, about 1 mL of blood was drawn from the vein under the wing of the duck with a disposable syringe, and injected into a 1.5 mL centrifuge that had been sterilized by high pressure and filled with about 200 μl of 2% sterile EDTA (Ethylene d...

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Abstract

The invention discloses an SNP molecular marker related to a cockscomb developmental character and a detection method thereof. A nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO:1, and the 302nd position of the SEQ ID NO:1 is G or A. The SNP molecular marker is related to the cockscomb developmental character and is a new molecular marker, by detecting the genotype of an SNP site of a to-be-detected chicken, early selection is conducted on the cockscomb developmental character, the production cost can be reduced, a genetic progress is accelerated, breeding of high-quality chickens can be better served, and the SNP molecular marker related to the cockscomb developmental character and the detection method thereof have a large economic value and scientific research value.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic engineering, and more specifically, relates to a SNP molecular marker related to comb development traits and a detection method thereof. Background technique [0002] For high-quality chickens, it is required that the broilers on the market have a sexually mature appearance such as large crown and red face, because only when they are close to sexual maturity, their fat deposits are more, the meat quality is the most delicious, and the flavor is the most intense. The development of cockscomb is the most obvious and important feature in the secondary sexual characteristics of chickens, which can be used as an index to measure the degree of sexual maturity, and is also an important index to distinguish high-quality chickens from fast-growing chickens after slaughtering and marketing. At the same time, there is also a great correlation between precociousness and chicken quality. The degree of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 束婧婷刘一帆邹剑敏章明屠云洁姬改革单艳菊巨晓军张笛盛中伟肖芹
Owner JIANGSU INST OF POULTRY SCI
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