Molecular marker capable of influencing intramuscular fat content of Duroc boar and application
A technology for intramuscular fat and molecular markers, which is applied in the field of molecular biotechnology and molecular markers, can solve the problems of limiting the application of molecular markers for complex traits and large confidence regions, and achieve the goal of increasing core competitiveness, improving economic benefits, and improving meat quality Effect
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[0035] 1. Experimental animals
[0036] The group of experimental pigs used in the present invention is 2296 purebred American Duroc of the Breeding Pig Branch of Guangdong Wens Food Group Co., Ltd., which is the core group of the Breeding Pig Branch.
[0037] In this experiment, Chinese and American Duroc pigs were selected from this resource group. The pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.
[0038] 2. Sample collection
[0039] The above-mentioned piglet docked tail and ear tissues were collected, soaked in 75% ethanol, and stored in a -20°C refrigerator for later use.
[0040] 3. 50K SNP typing in the whole pig genome
[0041] From the ear tissue or docked tail tissue collected from each individual of the 2296 Duroc species selected from the above resource groups, the whole genome DNA was extracted by the standard phenol-chloroform method, and each piece was accu...
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