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A Molecular Marker Related to Laying Duck Laying Performance and Its Application in Breeding

A molecular marker, egg-laying performance technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Inactive Publication Date: 2021-12-24
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the MTNR1C gene in laying ducks, so it cannot be applied to duck breeding

Method used

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  • A Molecular Marker Related to Laying Duck Laying Performance and Its Application in Breeding
  • A Molecular Marker Related to Laying Duck Laying Performance and Its Application in Breeding
  • A Molecular Marker Related to Laying Duck Laying Performance and Its Application in Breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Cloning, sequencing and polymorphism analysis of duck MTNR1C gene

[0019] A population of 785 Shaoxing ducks (Hubei Shendan Health Breeding Co., Ltd.) was selected for the experiment. After the pedigree data and egg-laying performance of the ducks were measured, about 1 mL of blood was drawn from the inferior jugular vein of ducks with a disposable syringe, and moved to the 10 % Anticoagulant (2% Na 2 -EDTA, 1.6% NaCl, pH 8.0) centrifuge tube, record the wing number, extract genomic DNA, and store at -80°C.

[0020] According to the sequence of duck MTNR1C gene (NCBI accession no. NW_004676748.1), the primer P-MTNR1C was designed, and the specific sequence is shown in SEQ ID NO.2 and SEQ ID NO.3.

[0021] PCR amplification was performed in duck genomic DNA using the above primers. The PCR reaction system was 25 μL, including 1 μL of DNA (50 ng / μL) template, 0.4 μL of upstream and downstream primers; 1.5 μL of dNTPs (10 mmol / L) mixture; 10× PCR buffer 2.5 μL; Mg 2+ (...

Embodiment 2

[0028] Association analysis of MTNR1C gene mutation sites with age at first lay and egg production:

[0029] In a population composed of 785 Shaoxing ducks, the association analysis of different genotypes of MTNR1C gene and duck egg-laying traits (including day-to-day, 34-week-old egg production and 72-week-old egg production) was conducted. The PCR-direct sequencing method established in Example 1 was used to carry out genotyping of individuals, and the relationship between egg-laying traits and genotypes was estimated by least squares method and tested for significance of difference using generalized linear model (GLM) of SPSS 18.0 software. .

[0030] The results of the association analysis are shown in Table 2.

[0031] Table 2 Correlation analysis between MTNR1C gene 108C>T base mutation and duck egg-laying performance

[0032]

[0033]

[0034] Note: Different letters a and b in the same column indicate significant difference (P<0.05); () indicates the number of ...

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Abstract

The invention relates to a molecular marker related to laying performance of laying ducks and its application in breeding, belonging to the technical field of molecular markers. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. In the sequence There is a C>T mutation at the 108th position of the duck, which is significantly correlated with the age at first laying, egg production at 34 weeks and egg production at 72 weeks, and the age at first laying of the TT genotype is significantly Lower than the CC and CT genotypes, the 34-week-old egg production of the TT genotype was significantly higher than that of the CC genotype, and the 72-week-old egg production of the TT genotype was significantly higher than that of the CC and CT genotypes. Sequence type, select TT genotype duck. The invention realizes the early selection and breeding of duck egg-laying traits, and provides powerful assistance for the breeding work of egg-type ducks.

Description

technical field [0001] The invention relates to a molecular marker related to egg-laying performance of laying ducks and its application in breeding, and belongs to the technical field of molecular markers. Background technique [0002] According to incomplete statistics, at present, the annual population of adult laying ducks in my country is about 200 million, the annual production of duck eggs is about 4 million tons, and the total output value of the laying duck industry exceeds 60 billion yuan. The laying duck industry has become one of the important industries in my country's rural economic development. one. Especially in the past 20 years, China's laying duck breeding industry has developed rapidly, and is developing from decentralized breeding to moderate scale operation. [0003] The egg-laying performance of laying ducks is affected by multiple factors such as heredity, environment, nutrition, and feeding management. Traditional breeding methods are mainly selected...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 曾涛卢立志唐建宏冯佩诗屈元启李国勤陈黎沈军达陶争荣田勇徐坚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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