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Gene chip, molecular probe combination and kit for analyzing characteristics of sheep wool and application

A technology of molecular probes and gene chips, which is applied in sheep detection and biological fields, can solve problems such as insufficient uniformity of sites, insufficient expression of functional sites and regions, etc.

Active Publication Date: 2021-08-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the existing sheep SNP chip is mainly based on the data of western sheep breeds, and lacks the SNP data of the combination of Chinese sheep breeds and foreign sheep breeds, there are still insufficient site uniformity, insufficient reflection of functional sites and regions, and more than 10 % of loci are very low frequency loci in Chinese sheep populations and many other problems, so it is of great significance to design a SNP chip that is suitable for Chinese sheep populations and can quickly and effectively detect sheep wool traits

Method used

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  • Gene chip, molecular probe combination and kit for analyzing characteristics of sheep wool and application
  • Gene chip, molecular probe combination and kit for analyzing characteristics of sheep wool and application
  • Gene chip, molecular probe combination and kit for analyzing characteristics of sheep wool and application

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Example 1 Obtaining the combination of SNP loci for hair traits

[0048] 1. Selection of individual sheep

[0049] In order to achieve a more comprehensive coverage of domestic and foreign sheep breeds, the applicant collected genetic information from 248 sheep individuals in Asia, Europe, Africa and the Middle East, including 16 Asian mouflon breeds and 172 local breeds And 60 breeds, specifically related to small-tailed Han sheep, Surabaya fur sheep, big-tailed Han sheep, Wadi sheep in Shandong, China, Hu sheep in Jiangsu, China, Tan sheep in Ningxia, China, Altay sheep and Bashbai sheep in Xinjiang, China , Dorper sheep, Chinese Merino sheep (fine-wool sheep), Chinese Merino sheep (super-fine wool sheep), Suffolk sheep, Zeller black sheep, Duolang sheep, Waggir Sheep sheep, Finland's Finnish sheep ( Finnsheep), Dutch Ouessant, Shetland, Solognote, Gotland, Drente Heathen, Bonga Sheep, Afar Sheep in Ethiopia, Mbororo Sheep in Niger, Yankasa Sheep in Nigeria, West Af...

Embodiment 2

[0062] The preparation of embodiment 2 primer combination and probe combination

[0063] Those skilled in the art design primers according to the sequence information of each site in the combination of SNP sites provided by the present invention, and use the designed primers for secondary structure evaluation and Tm value evaluation, and finally obtain good specificity, Primers that are highly sensitive and can achieve detection purposes under the same reaction conditions.

[0064] Wherein, secondary structure assessment and Tm value assessment can be carried out by any method commonly used in the art, for example, using DNA folding form to assess its secondary structure, see ( ), and then use the software RaW-Probe to evaluate its Tm value.

[0065] The above methods are all conventional methods. According to the site information in the combination of SNP sites for hair provided by this application, it can be obtained without creative labor. Therefore, according to the comb...

Embodiment 3

[0067] The preparation of embodiment 3 gene chips

[0068] The SNP gene chip of the present application adopts conventional method to immobilize the primers or probes obtained in Example 2 on polymer substrates, such as nylon membranes, nitrocellulose membranes, plastics, silica gel wafers, micro-magnetic beads, etc., or to immobilize the probes The primers or probes obtained in Example 2 are directly synthesized on a glass plate, or on a hard surface such as glass, and the use method of the SNP gene chip of the present application is the same as the conventional method.

[0069] It should be noted that those skilled in the art can use any method to prepare the SNP gene chip for detecting sheep wool, and can also entrust a biological company to prepare it, but the SNP gene chip prepared based on the combination of SNP sites for wool provided by this application Chips all belong to the protection scope of the present invention.

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Abstract

The invention discloses a gene chip, a molecular probe combination and a kit for analyzing the characteristics of sheep wool and application, and relates to the technical field of biology. A 2849-SNP-site combination for analyzing the characteristics of the sheep wool and the molecular probe combination, the gene chip and the kit prepared on the basis of the site combination are provided, the characteristics difficult to measure in the early stage are detected from the gene level, the breeding process can be accelerated, the breeding cost can be saved, and the molecular probe combination, the gene chip and the kit can be used for screening and identifying sheep varieties, reconstructing sheep pedigree, protecting germplasm resources and improving the germplasm resources.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the technical field of sheep detection, and more specifically to a gene chip for analyzing the traits of sheep wool, a molecular probe combination, a kit and its application. Background technique [0002] Sheep is one of the most important economic animals in the world, not only as food for human consumption, but also as a source of dairy products. In addition, its wool value also plays an important role in social development. With the continuous decline in the economic value of the wool textile market in recent years, the number of wool-based sheep in my country has continued to decrease, but the advantages of wool products still exist. In order to better analyze and study the wool performance of sheep and obtain better High-quality wool, and in order to protect sheep breeds, more and more researchers have used genetic analysis to study sheep wool traits. [0003] At present, molecu...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李孟华李心罗凌云杨继吕锋骅
Owner CHINA AGRI UNIV
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