Specific amplification primer of sika deer microsatellite site M027 and application of specific amplification primer

Abstract

The invention provides a specific amplification primer of a sika deer microsatellite site M027 and an application of the specific amplification primer, relates to the specific amplification primer ofthe sika deer microsatellite site M027 and the application of the specific amplification primer, and aims to provide the specific amplification primer of the sika deer microsatellite site M027 and theapplication of the specific amplification primer. The nucleotide sequence of the primer of the sika deer microsatellite site M027 is as shown in SEQ.ID.NO:1 and SEQ.ID.NO:2. The sika deer microsatellite site M027 is applied to analysis of the growth traits of cornu cervi pantotrichum. The growth traits of the cornu cervi pantotrichum are selected according to genotypes, high-yield trait cornu cervi pantotrichum individuals and low-yield cornu cervi pantotrichum individuals can be screened, the accuracy is high, the operation is simple, the expense is low, the selection of the growth traits ofthe cornu cervi pantotrichum can be started at the early stage of life of sika deer, the generation interval is shortened, the selection intensity is improved, and the breeding process of the sika deer is accelerated. The specific amplification primer disclosed by the invention is suitable for the field of breeding of the sika deer.

Description

technical field [0001] The invention relates to a specific amplification primer for sika deer microsatellite site M027 and application thereof. Background technique [0002] Sika deer antler, as a traditional and precious medical and health care medicinal material, has high economic value. At present, the key issue affecting the economic benefits of my country's sika deer breeding industry is to increase the production of antler. However, the high-yielding trait of velvet antler is controlled by multiple genes, and it is a quantitative trait with low heritability. Using traditional breeding techniques, the cycle is relatively long. For example, the breeding of Shuangyang sika deer takes up to 50 years, and it is difficult to carry out early selection. Therefore, at present, many scholars use molecular genetic markers to study the production traits of sika deer antler from the molecular level. Molecular genetic markers can effectively overcome the shortcomings of traditiona...

AI Technical Summary

Problems solved by technology

Due to the above reasons, no effective microsatellite molecular markers related to the production performance of sika deer antler production have been screened at home and abroad, which greatly limits the construction of the physical map of sika deer and further in-depth research on molecular breeding of sika deer for velvet.

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