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Molecular marker tightly linked with major QTL (Quantitative Trait Loci) of cotton seed oil content of upland cotton and application thereof

A technology of oil content and molecular markers, which is applied in the fields of cotton breeding and molecular biology, to improve breeding efficiency, reduce workload, and speed up selection progress

Inactive Publication Date: 2011-11-23
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the QTL of cottonseed oil content in upland cotton

Method used

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  • Molecular marker tightly linked with major QTL (Quantitative Trait Loci) of cotton seed oil content of upland cotton and application thereof
  • Molecular marker tightly linked with major QTL (Quantitative Trait Loci) of cotton seed oil content of upland cotton and application thereof
  • Molecular marker tightly linked with major QTL (Quantitative Trait Loci) of cotton seed oil content of upland cotton and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Molecular markers closely linked to the main QTL for cottonseed oil content in upland cotton were obtained by the following methods:

[0024] (1) Construction of population and determination of cottonseed oil content:

[0025] The high-oil upland cotton variety DPLSR3 was used as the female parent, and the low-oil upland cotton variety Yancheng 115 was used as the male parent. In 2008, a hybrid combination was established at the Lishui Plant Experimental Base of the Jiangsu Academy of Agricultural Sciences. At the end of the same year, Hainan Nanfan F 1 , selfing produces F 2 . In 2009, F 2 and parents, a total of 151 F 2 Single plant, harvested F 2 Seed, to F 2 The seeds were fluffed and dried, and F was measured by near-infrared analysis method (Xu Peng, et al. 2 oil content. 2010 Planting F 2∶3 Plant rows, harvest seeds according to plant rows, also develvet and dry the seeds, and measure F by near-infrared analysis method 2∶3 oil content.

[0026] (2) Gene...

Embodiment 2

[0029] Molecular markers closely linked to the main QTL for cottonseed oil content in upland cotton were verified in genetic populations:

[0030] The cottonseed oil content of the derivative lines of upland cotton variety DPLSR3 was screened by molecular marker TMB1216 with a size of 190bp:

[0031] Identification object:

[0032] The upland cotton variety DPLSR3 was crossed with Yancheng 115 and propagated to F 3 Generation, they are used as the identification object of cottonseed oil content.

[0033] Identification process

[0034] a) Identification by molecular markers closely linked to the major QTL for cottonseed oil content in upland cotton

[0035] Firstly, the DNA obtained by separating and extracting the leaves of each identification object by CTAB extraction method, and then using the primers 5'AATTTAGTTTCATACACAC3'(SEQ ID NO:1) and 5'TGGTCACCCATCGTTAAC3'(SEQ ID NO:2) of the molecular marker TMB1216 to target These DNAs were subjected to polymerase chain reacti...

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Abstract

The invention relates to a molecular marker tightly linked with a major QTL (Quantitative Trait Loci) of the cotton seed oil content of upland cotton and application thereof. The molecular marker tightly linked with the major QTL of the cotton seed oil content of upland cotton is TMB1216. The molecular marker TMB1216 can be applied to the genotype detection of an upland cotton variety or strain to judge the oil content of the variety or strain. In the invention, the molecular marker TMB1216 is used for assisting in selecting, so that high accuracy is achieved, the selecting speed is increased, the breeding workload is lowered greatly simultaneously, the breeding efficiency is increased, and the defects and disadvantages of long period, high subsequent screening workload, low verification efficiency of the variety or strain and the like existing in the conventional breeding are overcome.

Description

technical field [0001] The invention relates to a molecular marker closely linked with the main effect QTL of cottonseed oil content in upland cotton and its application, belonging to the fields of cotton breeding and molecular biology. Background technique [0002] As a by-product of cotton fiber production, cottonseed is also a good oil resource. Among edible oils, cottonseed oil has the highest linoleic acid content (Qiu Xinmian et al., 1999). The linoleic acid content of cottonseed oil is as high as 55.6%, which is a high-grade health care oil with extremely high linoleic acid content (Chen Xusheng et al., 2003). At the same time, as a raw material for biodiesel, cottonseed oil has the following advantages: (1) The carbon chain length of petrochemical diesel oil is C15-C18, while 99% of the carbon chain length of fatty acids in cottonseed oil is concentrated in C16 and C18, so cottonseed oil It has a good chemical basis for biodiesel; (2) Compared with rapeseed and soy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 徐鹏郭婷婷张香桂倪万潮郭书巧沈新莲
Owner JIANGSU ACAD OF AGRI SCI
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