Method for breeding new breed of pear of self-pollination and bear fruit
A new germplasm and self-flowering technology, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of late start, restriction of self-fertile varieties selection, and inability to screen out new germplasm, and achieve The effect of low investment and wide application prospects
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[0037] 1. Materials and methods
[0038] 1.1 Test material
[0039] The high-quality early-maturing pear variety 'Bali' or 'Changshilang' with known S genotype was selected as the female parent (♀), and the self-fertilizing variety 'Aoga 20th Century' was used as the male parent (♂) for cross-pollination test. Because the S genotype of 'Bali' is S 2 S 5 , the S genotype of 'Changjuro' is S 2 S 3 , they all contain an S 2 Gene, so that the "August 20th Century" containing S 2 The pollen of the gene cannot be fertilized, only S 4 sm Pollen completes fertilization, and all offspring contain S 4 sm Gene, therefore, the progeny of these hybrid combinations are self-fertile.
[0040] 1.2 Hybrid combination
[0041] 1) Combination 1: A hybrid combination with 'Bali' as the female parent (♀) and the autofertile variety 'Aoga 20th Century' as the male parent (♂). The hybrid combination obtained a total of 6547 hybrid seeds, and the hybrid was stratified and seeded to obtai...
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