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A kind of Phellinus sporocarpa mycelia separation method

A technology of Phellinus linteus and a separation method, which is applied in the field of separation of medicinal bacteria, can solve the problems of cumbersome operation of tissue block extraction, large repetitive workload, and high contamination rate of miscellaneous bacteria, and achieves difficult disinfection and thorough, simple method, and dyeing. low impurity effect

Active Publication Date: 2017-10-20
陈卫华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The key is to disinfect the fruiting bodies with chemical agents. Because the fruiting bodies of Phellinus fungus are very hard and lignified, the disinfection of the fruiting bodies of Phellinus fungus and the operation of taking pieces from tissues are very cumbersome, and it is not easy to disinfect thoroughly. The mycelium obtained from Phellinus sporocarpa by tissue separation method has a very high rate of contamination by bacteria, a very low success rate, and a large amount of repetitive work

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take the complete Phellinus sporophores (radius 3-4cm, thickness 2-3cm) into a wide-mouth culture bottle (diameter 7.5cm, height 12cm), add 120ml of tap water, then tightly wrap the mouth of the bottle with 3 layers of newspaper, heat culture Boil the water in the bottle and keep it for 10 seconds. After cooling, incubate at 35-37°C for 8-12 hours, take it out, and repeat the above boiling and cultivation for 2 times; , cultivated under the condition of 25-28 ℃ for 72 hours to grow 1cm mycelium on the Phellinus sporocarp, at this moment it can be taken out and separated from the mycelia.

Embodiment 2

[0022] Put the complete Phellinus sporocarp (radius 3-4cm, thickness 2-3cm) into a wide-mouth culture bottle (diameter 7.5cm, height 12cm), add pure water to make it submerged Phellinus sporocarp, use 3 layers Tightly wrap the mouth of the bottle with newspaper, then boil and keep for 5-15 seconds, then incubate at 30-37°C for 12-18 hours, repeat the above steps of boiling and incubating for 2 times, and finally boil for 10 seconds, after cooling, at 25 Cultivate under the condition of -28°C for 48 hours, that is, a hyphae of about 0.5 cm grows on the fruiting body of Phellinus fungus, and the hyphae can be separated.

Embodiment 3

[0024] Put the complete Phellinus sporophores (radius 3-4cm, thickness 2-3cm) into a wide-mouth culture bottle (diameter 7.5cm, height 12cm), add distilled water to immerse, tightly wrap the mouth of the bottle with 3 layers of newspaper, then boil and dry Keep it for 15-20 seconds, then incubate at 32-35°C for 18-24 hours, repeat the above steps of boiling and culturing twice, and finally boil for 15 seconds, cool down, and incubate at 25-30°C for 60 hours , that is, about 0.8cm mycelium grows on the Phellinus sporocarp, which can be separated from the mycelium.

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Abstract

The invention discloses a phellinus linteus sporocarp hypha separating method. The method comprises the steps of placing complete phellinus linteus sporocarp into a culture bottle; adding liquid until the phellinus linteus sporocarp is immersed; boiling and maintaining for 5 to 20 seconds; culturing for 8 to 72 hours at the temperature of 25 to 37 DEG C; repeating the boiling and culturing processes for a plurality of times until hypha grows in the phellinus linteus sporocarp; separating hypha. Compared with the traditional tissue separation method, the phellinus linteus sporocarp hypha separating method has the characteristics that the raw materials are easily obtained, the preparation is convenient, special devices are saved, the contamination rate in operation is low, the success rate is high, the hypha can grow quickly, and the culture costs a little time; the method is very suitable for field separation of the phellinus linteus sporocarp hypha.

Description

technical field [0001] The invention relates to a method for separating the mycelia of fruiting bodies, in particular to a method for separating the mycelia of the fruiting bodies of Phellinus fungus, and belongs to the field of separation of medicinal fungus strains. Background technique [0002] Phellinus Basidiomycota ( Basidiomycotas ), Laminaria ( Hymenomycetes ), Aphylloides ( Aphyllophorales ), Porinaceae ( Hymenochae taceae ), Acupuncture ( Phellinus Quel .), commonly known as Phellinus because of its bright yellow fruiting body, is a rare medicinal fungus. Phellinus is one of the best anti-tumor fungi internationally recognized, without any toxic effects, and is a precious medicinal fungus with great development value. Due to the limited resources of wild Phellinus japonica, people have started artificial cultivation, but whether it is artificial cultivation or liquid fermentation mycelium production, obtaining high-quality excellent fungus mycelium is the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 邵伟唐明熊泽
Owner 陈卫华
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