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175 results about "Phellinus linteus" patented technology

Phellinus linteus (Japanese "meshimakobu", Chinese "song gen", Korean "sanghwang", English "Mesima", American English "black hoof mushroom") is a medicinal mushroom used in Japan, Korea and China for centuries to prevent ailments as diverse as gastroenteric dysfunction, diarrhea, haemorrhage and cancers.

Artificial cultivation method of Phellinus linteus

The invention discloses an artificial cultivation method for Phellinus baumii Pilat. The method comprises the following steps: (1) inoculating the purified strain separated from a Phellinus baumii Pilat fruiting body to a mother seed culture medium so as to obtain a mother seed; (2) inoculating the mother seed to an original seed culture medium so as to obtain a Phellinus baumii Pilat original seed; (3) inoculating the Phellinus baumii Pilat original seed to a cultivar culture medium inside a cultivation bag for culture; and (4) transplanting the cultivation bag fully covered by hypha into a mushroom producing room and carrying out the mushroom producing culture under the following conditions: 85 to 95 percent of relative air humidity and 22 to 25 DEG C of temperature. The method finds out the best environmental factors such as the relative air humidity, the temperature, the illumination, and the like for the growth of the Phellinus baumii Pilat fruiting body. Through the artificial accurate control on the factors of relative humidity and temperature in the mushroom producing room, the method can maximally improve the growth speed of the Phellinus baumii Pilat fruiting body while simultaneously ensuring the quality of the Phellinus baumii Pilat fruiting body. The method has the advantages of high yield, high Phellinus baumii Pilat fruiting body quality, material conservation, short production cycle, and the like.
Owner:BEIJING FORESTRY UNIVERSITY +1

Artificial cultivation method for phellinus linteus

The invention relates to an artificial cultivation method for phellinus linteus. Artificial domestication cultivation research of the phellinus linteus newly starts in China, a few exploration tests are conducted in parts of areas, and the problems such as sporocarp is low in productivity and poor in quality (unshaped) exist mainly due to the fact that cultivation techniques are immature. The artificial cultivation method for the phellinus linteus comprises the steps that phellinus linteus strains are activated, 0.3 square centimeter stain blocks are taken on the aseptic condition to be inoculated to phellinus linteus cultivation culture media in cultivation bags, the stain blocks are cultivated at constant temperature being 28 DEG C without light until mycelia grow all over the cultivation bags, and cultispecies are obtained; when the mycelia in the cultivation bags are yellow or have protuberant nodular primordia, the cultivation bags are moved out of yellow areas, and semi-bag-off soil-cast yellow-out cultivation is conducted. According to the artificial cultivation method for the phellinus linteus, mulberry fields are used for phellinus linteus cultivation, and growing conditions of generation, differentiation and development of wild phellinus linteus sporocarps are imitated in nature. The phellinus linteus is high in productivity, good in quality (similar to wild phellinus linteus in shape), short in growth cycle, simple in cultivation mode, and capable of being cultivated in large scale.
Owner:MICROBIOLOGY INST OF SHAANXI

Biologic compound amylose nutrient fluid with high efficiency and preparing method thereof

The invention relates to a preparation method of a biological composite polysaccharide efficient nutrient solution. The invention comprises the steps of selecting a strain, preparing a nutrient, culturing a liquid spawn, generating a mycelium in a fermentor, filtrating the fermentation broth and generating the nutrient solution which is rich in microbial polysaccharide after concentration. The key of the invention lies in that the composite nutrient solution is made by mixing the mycelium culture medium extracted from the respective fermentor after deep fermentation of the following microbial strains after the above steps according to the following weight proportion: mythic fungus of 5 to 20 percent, aweto of 5 to 20 percent, poria of 5 to 15 percent, grey polypore of 5 to 15 percent, boletus of 5 to 15 percent, phellinus linteus of 5 to 10 percent, manyzoned polypore of 5 to 20 percent, mushroom of 6 to 20 percent, bailing of 5 to 10 percent mushroom and agaricus blazei strain of 5 to 20 percent. The nutrient solution is flask separated, sealed and sterilized under bacteria free environment to become the final product.
Owner:张真元

Phellinus igniarius bacterial strain liquid fermenting culture medium and method for fermenting and producing phellinus linteus polysaccharides

The invention relates to a phellinus igniarius bacterial strain liquid fermenting culture medium and a method for fermenting and producing phellinus linteus polysaccharides. The culture medium has a prescription shown as follows: 20 to 30g/L of glucose, 2 to 6g/L of yeast powder, 1 to 3g/L of KH2PO4, 0.3 to 0.7g/L of MgSO4.7H2O, 110mg/L of VB, and 1L of water; a pH is under a natural condition; a fermenting condition is that a temperature is at 28 to 32 DEG C, a rocking speed is at 140 to 180rpm, inoculation amount is 20 to 30%, cultivation is carried out for 9 to 11 days; the method comprises the following steps of: centrifuging, collecting fermenting liquid, washing via distilled water, precipitating to obtain a mycelium, then extracting polysaccharide inside or outside cells. With the adoption of the phellinus igniarius bacterial strain liquid fermenting culture medium and the method for fermenting and producing the phellinus linteus polysaccharides provided by the invention, yield of the mycelium of phellinus igniarius and content of the polysaccharide inside or outside the cells can be obviously increased; moreover, yields of the phellinus linteus polysaccharides is 6.34% higher than that of polysaccharide extracted by a common hot water extracting method. An animal test proves that extracted phellinus linteus polysaccharides have obvious biological activity such as tumour resistance, oxidization resistance, and improvement of immunity.
Owner:FUJIAN AGRI & FORESTRY UNIV

Artificial nutrient medium for Phellinus linteus fluid culture and method for fermentation of Phellinus linteus polysaccharide

The invention provides a synthetic medium for culturing a phellinus igniarius liquid. The synthetic medium takes glucose as a carbon source and 20 kinds of amino acid as a nitrogen source, and consists of inorganic salt and vitamin. The ratio of carbon to nitrogen is designed as 50 to 1; and the specific concentration g/l is as follows: the glucose: 30 to 80 grams; glutamic acid: 0.1 to 1.5 grams; glutamine: 0.1 to 1.5 grams; aspartic acid: 0.1 to 1.5 grams; asparagines: 0.1 to 1.5 grams; glutamic acid: 0.01 to 0.1 gram; tryptophan: 0.01 to 0.1 gram; valine: 0.01 to 0.1 gram; alanine: 0.01 to 0.1 gram; etc. During the whole culture process of the synthetic medium, phellinus igniarius mycelium is quick in growth; the yield of the phellinus igniarius mycelium can reach 20 grams per liter to the highest degree; simultaneously no agricultural and sideline product is introduced as the carbon source and the nitrogen source; substances such as proteins in the medium, medium compositions and so on are not carried; phellinus igniarius exopolysaccharide produced through fermentation has high purity and stable quality; and the yield of phellinus igniarius polysaccharide can reach 0.198 gram per liter to the highest degree. The synthetic medium has the advantages of clear compositions, stable quality and so on, and can be used for mass culture of the phellinus igniarius liquid and research of the regulation and control rule of polysaccharide anabolism.
Owner:SOUTHWEST UNIVERSITY

Method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature

InactiveCN104592409AIncrease productionProtect active structureAlcoholUltrasonic assisted
The invention discloses a method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature. The method comprises the following steps: mixing male agaric mycelium obtained by a liquid fermentation method with deionized water evenly at the feed-to-liquid ratio of 1g to 40-60mL; extracting at -0.04MPa to -0.07MPa and 50-60 DEG C for 1-3 hours; and centrifuging and separating an extract liquid, and carrying out alcohol precipitation to obtain active phellinus linteus polysaccharide. The extraction method disclosed by the invention is simple, low in extraction cost, and easy to operate. Compared with a hot water extraction method and an ultrasonic assist extraction method, the yield of the phellinus linteus polysaccharide is increased; the active structure of the phellinus linteus polysaccharide can be well kept; the high-activity phellinus linteus polysaccharide is obtained.
Owner:SHAANXI NORMAL UNIV

Phellinus linteus strain and breeding method thereof

The invention provides a phellinus linteus strain and a breeding method of the phellinus linteus strain. The breeding method comprises the steps of preparation and mutagenesis of protoplast, screening of a mutagenesis strain, and verification of the mutagenesis strain. The shake flask biomass of the phellinus linteus strain obtained through the breeding method reaches 17.92 g / L and is higher than that of an original strain by 9.51%. The yield of flavone reaches 0.47 g / L and is higher than that of the original strain by 52.16%, and the yield of polysaccharide reaches 3.75 g / L and is higher than that of the original strain by 67.95%. The biological yield and the yield of metabolic active matter are also remarkably higher than those of the parent strain SH1, and the free radical scavenging capability is remarkably improved.
Owner:SHANGHAI ACAD OF AGRI SCI

Preparation method of zinc-enriched liquid fermentation product of phellinus linteus

ActiveCN104372061AIncrease contentHigh activityMicroorganism based processesFermentationExtremely low frequency magnetic fieldZinc
The invention discloses a preparation method of a zinc-enriched liquid fermentation product of phellinus linteus. The preparation method comprises the following steps: inoculating a phellinus linteus strain onto a liquid seed culture medium added with zinc-enriched yeast and a diaphragma juglandis extract, rapidly culturing with the help of a low-frequency ultrasound with a certain frequency and intensity, inoculating the phellinus linteus liquid strain onto a liquid fermentation culture medium added with zinc-enriched yeast and a plukenetia volubilis leave enzymolysis product and culturing in an ultra-low-frequency magnetic field with a certain frequency and intensity to obtain the zinc-enriched liquid fermentation product of phellinus linteus. According to the preparation method, by the physical-assisted synergistic fermentation of seed liquid and fermentation two-phase low-frequency ultrasound and ultra-low-frequency magnetic fields and the synergistic effect of exogenous additives such as the diaphragma juglandis extract and the plukenetia volubilis leave enzymolysis product, the contents and activities of organic zinc and zinc polysaccharide in final fermentation product of phellinus linteus are significantly increased.
Owner:ZHEJIANG FORESTRY ACAD

Preparation method of composite solid-state probiotic agent including phellinus linteus

The invention relates to a preparation method of a composite solid-state probiotic agent including phellinus linteus. The method comprises the following steps of inoculating phellinus linteus seed fluid to solid-state fermentation culture mediums, performing fermentation, and degrading cellulose and hemicellulose type substances for producing monosaccharide; and then sequentially adding after fermentation bacterial fluid for a plurality of times, performing fermentation to obtain fermentation products, and then drying the fermentation products so as to obtain the composite solid-state probiotic agent. According to the preparation method disclosed by the invention, stagewise solid-state fermentation is performed according to requirement differences of different probiotics for nutrient substances, so that the culture mediums can be efficiently decomposed; phellinus linteus grows through solid-state substrates to produce active polysaccharide, and besides, the cellulose and hemicellulose type substances are degraded to generate the monosaccharide; and the solid-state probiotic agent has special paste fragrance, contains fungus polysaccharide, is high in effective viable count, is high in the content of organic nitrogen, and has the efficacy of adjusting the flora in the intestinal tracts of animals, enhancing immunity, increasing the utilization rate of the solid-state substrates, reducing the fetor of excrement of livestock and improving environment.
Owner:INNER MONGOLIA UNIVERSITY +1

Method for enriching and purifying common phellinus fungus general flavone in common phellinus fungus

The invention belongs to the field of natural organic chemistry and relates to a method for enriching and purifying common phellinus fungus general flavone in common phellinus fungus by using a batch distillation-macroporous absorption resin coupling method. The method is characterized in that the common phellinus fungus general flavone is separated and purified by applying a macropore adsorption technology, the adsorption selectivity to the common phellinus fungus general flavone is better, the separation effect is remarkable, the extraction purity is high, the semi-finished product with more than 35 percent of the common phellinus fungus general flavone and the final product with above 90 percent of the common phellinus fungus general flavone can be obtained, and the defects of relatively lower conventional extraction rate and low extraction purity are overcome. Macroporous absorption resin with stable physicochemical property, large surface area, higher switching speed, high mechanical strength, strong contamination resistance and good thermal stability is selected, and the common phellinus fungus general flavone is selectively absorbed from a solution through physical adsorption, thus the method has the advantages of high adsorption, high deabsorption speed and larger adsorption capacity.
Owner:DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD

Mushroom concentrated liquid and drink containing beta-glucan and preparation method thereof

The invention discloses mushroom concentrated liquid and drink containing beta-glucan, which is liquid type health-care drink prepared by mixing 5-50 percent of concentrated extract liquid of nine mushrooms of birch mushroom, phellinus linteus, ganoderma lucidum, grifola frondosa, hericium erinaceum, shiitake mushroom, sparasis crispa(Wulf)Fr., pine mushroom and aweto, 1.6-3 percent of sugar or sugar alcohol, 2.0-3.5 percent of honey, 0.5-0.8 percent of syrup and 0.01-0.03 percent of acid with water till 500ml. The drink is easy to be internally digested and absorbed by a human body, can improve the immunity resisting ability of the human body, and has the advantages of rich nutrition, enhancement and improvement of human immunity, virus resistance, fungus resistance, reduction of blood sugar, stimulation of hematopoiesis, reduction of cholesterin, reduction of blood lipid, aging resistance and the like, and can be used as a novel health-care type drink. The invention further discloses a method for preparing the mushroom concentrated liquid and drink containing delta-dextran.
Owner:文承官 +1

Korean phellinus linteus plastic bag cultivation method

The invention relates to a cultivation technology of fungi and in particular relates to a Korean phellinus linteus plastic bag cultivation method. The method comprises the following steps: adding a culture medium into a stirrer according to a formula ratio, and adding water for stirring; bagging by using polypropylene edible mushroom bags; sterilizing by using constant-pressure steam; reducing the temperature to 45-60 DEG C, and transferring into a sterile room for stacking, cooling to the temperature of 25-30 DEG C, and inoculating under sterile conditions; transferring into a culture room after inoculating, wherein the edible mushroom bags are full of mycelia within 30-40 days; transferring the mushroom bags full of mycelia into a fruiting room to enter a fruiting body cultivation stage, arranging the mushroom bags, and opening the bag cover until yellow mushrooms come out; picking the fruiting bodies within 120-180 days after maturation, wherein the picking manner is that the mushroom bag is held by one hand and the fruiting bodies are picked by the other hand; and drying and packaging. According to the method disclosed by the invention, the fruiting bodies of the Korean phellinus linteus and pharmacological active ingredients of the mycelium are maintained to the greatest degree; and by experiencing the method, the content of cultivated fruiting bodies and various components of the mycelium is equivalent to content of various components of wild phellinus linteus.
Owner:徐振宗

Method for performing large-scale and stable cultivation on phellinus linteus

ActiveCN107142216AGood effectProtection from Unstable InterferenceFungiMicroorganism based processesHigh pressureCulture mediums
The invention belongs to the technical field of phellinus linteus cultivation, and provides a method for performing large-scale and stable cultivation on phellinus linteus. The method comprises the following steps: compounding zeolite powder and diatomite, performing high-temperature sterilization, rapidly cooling to a certain temperature by utilizing liquid nitrogen, and inoculating the phellinus linteus into a complexing body of the zeolite powder and the diatomite in a sterile room; prefabricating a culture medium by using water, mulberry sawdust, a carbon source, a nitrogen source, a phosphorus fertilizer, a potassium fertilizer, magnesium sulfate and agar powder, and performing sterilization by using high-pressure steam; mixing the complexing body inoculated with the phellinus linteus and a nutritional medium according to a proportion, putting the mixture into a grid plate, packaging by using plastic, putting into a culture room, controlling the temperature and the relative humidity of the culture room, maintaining a dark state, and cultivating for a certain time to obtain uniform mycelium. When the method is used for cultivating the phellinus linteus, the survival rate is high, the quality is stable and large-scale cultivation can be realized.
Owner:金华市挺美科技有限公司

Preservation method of phellinus linteus

The invention relates to a preservation method of phellinus linteus. A main material of a culture medium is prepared from the following components by mass: 50% of tung tree wood dust, 15% of maple tree wood dust, 25% of wheat bran, and 10% of corn cob powder; an auxiliary material is prepared from the following components by mass: 16% of magnesium sulfate, 34% of potassium dihydrogen phosphate, and 50% of gypsum powder; a formula of the culture medium of a wood dust tube comprises the main material, the auxiliary material and water according to a ratio of 1:0.003:1.4. The preparation method comprises the following steps of firstly, uniformly mixing the two types of wood dust in the main material, sequentially adding the wheat bran and the water, and uniformly mixing; adding the mixed auxiliary material, uniformly mixing, adding water and uniformly stirring; separately loading the culture medium into test tubes, packaging, sterilizing, and inoculating, so as to obtain the phellinus linteus preservation strain when the phellinus linteus can well grow and be free from infection by infectious microbes after re-transfer verification. The preservation method has the advantages that the effect is excellent, and the preserved strain can be effectively preserved for 2 to 5 years; after activating, the growth rate of hypha, growth condition, biology conversion rate and the like are basically free from influence.
Owner:HUNAN INST OF MICROBIOLOGY

Traditional Chinese medicine hair-blacking and hair-smoothing agent and preparation method thereof

The invention discloses a traditional Chinese medicine hair-blacking and hair-smoothing agent. The agent is prepared from, by weight, 20-40 parts of radix rehmanniae preparata, 15-30 parts of radix polygonum multiflorum preparata, 22-30 parts of herba ecliptae, 20-30 parts of gynostemma pentaphyllum, 20-30 parts of fructus ligustri lucidi, 18-28 parts of rhizoma chuanxiong, 18-28 parts of angelicasinensis, 5-10 parts of dried ginger, 5-12 parts of meadowfoam seed oil, 6-12 parts of lophatheri herbak, 5-10 parts of phellinus linteus, 3-8 parts of ganoderma, 5-10 parts of Sarcodon imbricatus, 5-10 parts of arimillaria mellea, 6-12 parts of gulfweed and 5-15 parts of black mulberries. The invention further provides a preparation method of the traditional Chinese medicine hair-blacking and hair-smoothing agent. The traditional Chinese medicine hair-blacking and hair-smoothing agent has the good permeation promoting capacity, active ingredients in the product can reach deep skin tissue bymore rapidly permeating through fur and can provide adequate nutrients for the fur, metabolism is promoted, the product effect is better, and the effects of blacking hair, nourishing hair, protectinghair and achieving moisturizing and maintaining are achieved.
Owner:广西和桂集团有限公司
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