Culture medium applicable to liquid fermentation production of phellinus linteus

Abstract

The invention in particular relates to a culture medium applicable to liquid fermentation production of phellinus linteus. The culture medium is characterized by being prepared from the following raw materials in percentage by weight: 6 percent to 8 percent of wheat bran, 2 percent to 4 percent of corn starch, 1 percent to 3 percent of peptone, 0.05 percent to 0.15 percent of MgSO4.7H2O, 0.10 percent to 0.15 percent of KH2PO4, 0.02 percent to 0.04 percent of K2HPO4.3H2O and the balance of water. According to the culture medium provided by the invention, the raw materials are convenient to select and the cost is low; a formula obtained by research can be used for obtaining high yield of phellinus linteus mycelia and extracellular active substances. The yield of the mycelia reaches 4.6217g / 100mL (46.217mg / mL). The yield of extracellular flavone reaches 16.198mg / mL. The yield of extracellular triterpene compounds reaches 5.736mg / mL.

Description

[0001] (1) Technical field [0002] The invention relates to Phellinus Phellinus, in particular to a medium suitable for the production of Phellinus Phellinus liquid fermentation. [0003] (2) Background technology [0004] The medicinal fungus Phellinus has anti-cancer, anti-oxidant, enhanced immune regulation, anti-inflammatory, hypolipidemic, and anti-viral effects. In traditional Chinese medicine, it is used to treat wounds, abdominal pain, and gonorrhea. Recent studies have highlighted its anticancer and liver protective properties. It contains active ingredients such as triterpenoids, flavonoids, and polysaccharides. [0005] At present, most of Phellinus chinensis products depend on the fruiting bodies of Phellinus chinensis, and the growth of the Phellinus chinensis fruiting bodies is highly dependent on the environment, the growth cycle is long, and the yield is unstable. The use of liquid culture can easily control and optimize various growth factors to obtain stabl...

AI Technical Summary

Problems solved by technology

Studies have reported that the yield of mycelium obtained by using liquid medium (potato / glucose broth 24%, malt extract 10%, peptone 1%) is 1.42 g / 100mL. This medium lacks mineral elements, which is not conducive to mycelium growth

Another study pointed out that the use of liquid medium (corn flour 50 g / L, wheat bran 50 g / L, KH 2 PO 4 2 g / L, MgSO 4 1 g / L, vitamin B1 300µg / L and vitamin B2 400µg / L) to obtain a mycelium yield of 0.356 g / 100mL, this formula contains low nitrogen, which is not conducive to the growth of mycelium, and the use of vitamins will increase its production cost

[0006] For the liquid culture of Phellinus, the current technology shows that the yield is generally low, or the cost of raw materials is high