Preparation method of composite solid-state probiotic agent including phellinus linteus
A probiotic agent, the technology of Phellinus linteus, applied in the application, animal feed, animal feed and other directions, can solve the problems of insufficient decomposition and utilization of nutrients, unable to produce ideal effects, and low content of beneficial microorganisms, so as to improve the palatability of feeds. Sex, ease of operation, the effect of regulating immunity
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Embodiment 1
[0027] This embodiment is a method for preparing a composite solid probiotic containing Phellinus igniarius. The method uses Phellinus igniarius seed liquid inoculated into a solid fermentation medium for fermentation to degrade cellulose and hemicellulose. Sugar; then add the post-fermentation broth in 2 times, carry out a sequential post-fermentation process, obtain the fermentation product after the fermentation, and dry the fermentation product to obtain a composite solid probiotic. The post-fermentation bacteria liquid is Bacillus, Saccharomyces cerevisiae and lactic acid bacteria.
[0028] The mass parts of each component in the solid fermentation medium are: 20 parts of corn flour, 35 parts of corn husk, 7.5 parts of soybean meal, 10 parts of soybean hulls, 20 parts of bran, 3 parts of corn gluten meal, 2.5 parts of talcum powder, Mongolian 0.2 parts of de-stone and 1.8 parts of molasses.
[0029] The process of inoculating Phellinus igniarius into the solid fermentation me...
Embodiment 2
[0045] On the basis of Example 1, a preferred embodiment of the present invention is a preparation method of a composite solid probiotic containing Phellinus igniarius:
[0046] The mass parts of each component in the solid-state fermentation medium are: 17 parts of corn flour, 39 parts of corn husk, 5 parts of soybean meal, 13 parts of soybean hulls, 16 parts of bran, 4.5 parts of corn gluten meal, 1 part of talcum powder, Mongolian 0.3 part of de-stone and 0.8 part of molasses.
[0047] The process of inoculating Phellinus igniarius into the solid fermentation medium for fermentation is as follows: After the solid fermentation medium is sterilized, the Phellinus igniarius seed solution is connected with an inoculum of 5% by volume, and the water content of the culture medium is adjusted to 85%. Solid-state fermentation at 25°C for 8 days; after the fermentation is completed, the resulting solid-state fermentation medium is stirred and mixed, and the mycelium is evenly dispersed i...
Embodiment 3
[0056] On the basis of Example 2, a preferred embodiment of the present invention also includes a preparation method of a composite solid probiotic containing Phellinus linteus:
[0057] The mass parts of each component in the solid fermentation medium are: 23 parts of corn flour, 31 parts of corn husk, 9 parts of soybean meal, 7 parts of soybean hulls, 24 parts of bran, 1.5 parts of corn gluten meal, 4 parts of talcum powder, Mongolian 0.1 part of stone removal and 2.8 part of molasses.
[0058] The process of inoculating Phellinus igniarius into the solid fermentation medium for fermentation is as follows: after the solid fermentation medium is sterilized, 20% of the volume of the inoculum is connected to the Phellinus igniarius seed solution, and the water content of the culture medium is adjusted to 40%. Solid-state fermentation at 35°C for 3 days; after the fermentation is completed, the resulting solid-state fermentation medium is stirred and mixed, and the mycelium is evenly...
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