169 results about "Probiotic agent" patented technology

Disclosed is a method of diagnosing irritable bowel syndrome, fibromyalgia, chronic fatigue syndrome, depression, attention deficit / hyperactivity disorder, autoimmune diseases, such as multiple sclerosis and systemic lupus erythematosus, or Crohn's disease, which involves detecting the presence of small intestinal bacterial overgrowth (SIBO) in a human subject having at least one symptom associated with a suspected diagnosis of any of those diagnostic categories. Also disclosed is a method of treating these disorders, and other disorders caused by SIBO, that involves at least partially eradicating a SIBO condition in the human subject. The method includes administration of anti-microbial or probiotic agents, or normalizing intestinal motility by employing a prokinetic agent. The method improves symptoms, including hyperalgesia related to SIBO and disorders caused by SIBO. Also disclosed is a kit for the diagnosis or treatment of irritable bowel syndrome, fibromyalgia, chronic fatigue syndrome, depression, attention deficit / hyperactivity disorder, autoimmune diseases, or Crohn's disease.

Methods of identifying subjects at increased risk of cancer, based upon detection of biofilms and / or biofilm-associated microbes within a subject, are disclosed. Therapies designed to prevent formation and / or reduce the size of biofilms in a subject identified to be at increased risk of cancer based upon detection of biofilms and / or biofilm-associated microbes are disclosed. In particular embodiments, the invention provides for identification of a subject at elevated risk of developing or having colorectal cancer and / or a colorectal adenoma, based upon detection of a biofilm and / or biofilm-associated bacteria within the gastrointestinal tract of the subject (optionally, within a biopsy specimen and / or stool sample of such subject). Therapies involving administration of an antibiotic agent and / or a probiotic agent to a subject, to prevent or reduce biofilm formation within the gastrointestinal tract of the subject, optionally provided in combination with additional cancer therapy, are also disclosed.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

Disclosed is a method of diagnosing irritable bowel syndrome, fibromyalgia, chronic fatigue syndrome, depression, attention deficit / hyperactivity disorder, autoimmune diseases, such as multiple sclerosis and systemic lupus erythematosus, or Crohn's disease, which involves detecting the presence of small intestinal bacterial overgrowth (SIBO) in a human subject having at least one symptom associated with a suspected diagnosis of any of those diagnostic categories. Also disclosed is a method of treating these disorders, and other disorders caused by SIBO, that involves at least partially eradicating a SIBO condition in the human subject. The method includes administration of anti-microbial or probiotic agents, or normalizing intestinal motility by employing a prokinetic agent. The method improves symptoms, including hyperalgesia related to SIBO and disorders caused by SIBO. Also disclosed is a kit for the diagnosis or treatment of irritable bowel syndrome, fibromyalgia, chronic fatigue syndrome, depression, attention deficit / hyperactivity disorder, autoimmune diseases, or Crohn's disease.

Disclosed is a method of manipulating the rate of gastrointestinal transit in a mammalian subject Also disclosed is the use, in the manufacture of a medicamens for the treatment of constipation, of a selective inhibitor of methanogensis, a methanogen-displacing probiotic agent, or a prebiotic agent that inhibits the growth of methanogenic bacteria or promotes the growth of competing non-methanogenic intestinal flora. Alternatively, in accordance with the invention, is disclosed the use in the manufacture of a medicament for the treatment of diarrhea, of methane, or a methane precursor, a methanogenic or other methane-enhancing probiotic agent, or a methanogenesis-enhancing prebiotic agent.

The invention discloses a probiotics microbial agent for regulating livestock intestinal flora and a preparation method thereof. The probiotics microbial agent is prepared by using candida utilis, streptococcis faecium, bacillus subtilis, bacillus natto, lactobacillus plantarum and rhodopseudomonas palustris as raw materials, performing the three-stage liquid fermentation, then uniformly mixing the enlarged cultivated fermenting bacteria with a feed wheat bran, performing the solid fermentation, drying at low temperature to obtain the probiotics agent for regulating the livestock intestinal flora structure. The agent can be used for regulating the livestock intestinal flora structure, promoting the growth of the probiotics, inhibiting the growth of the harmful bacteria, substituting the antibiotics, improving the feed utilization rate, obviously reducing the odor of the excrement of the livestock and improving the ecological environment of aquiculture and round environment, and is efficient, non-toxic and no residue. The probiotics microbial agent can be widely applied to a livestock feed additive so as to prevent the livestock intestinal disease and respiratory disease.

The invention provides application of a compound probiotic agent in preparing products for treating ulcerative colitis. The compound probiotic agent contains four probiotic lactic acid strains, namelylactobacillus casei Zhang, bifidobacterium zoonosus V9, lactobacillus plantarum P-8 and lactobacillus plantarum C2, and the probiotics all have good acid resistance, artificial gastrointestinal fluidtolerance and bile salt tolerance, and do not carry drug-resistant plasmids. The four probiotic strains are high in safety. The compound probiotic agent further contains prebiotics which can synergize with the probiotics, and then the probiotic function of the probiotics is ensured. Accordingly, the compound probiotic agent has remarkable curative effect on the ulcerative colitis, the probiotic function of an intestinal flora can be adjusted so as to make the intestinal flora healthy, and by taking the compound probiotic agent as an adjuvant drug for treating the ulcerative colitis, the compound probiotic agent has the advantages of being simple in treatment, high in remission rate, free of toxic or side effects and the like.

The invention belongs to the technical field of fertilizers and particularly relates to an anti-disease composite microbial fertilizer specially-used for American ginseng and also relates to the preparation method of the microbial fertilizer. The microbial fertilizer includes furfural residue, cassava residue, decayed cow dung, lignite humic acid, plant ash, tea residue, clay, mineralized yellow-brown potassium humate and a composite probiotic agent. The microbial fertilizer is balanced in nutrients and is abundant in microelements and can effectively increase productivity and improve fertility level of soil. The added composite probiotic agent can significantly inhibit the main diseases of the American ginseng, such as cylindrocarpon destructans diseases, root rot, damping off, black spot and the like. By means of the composite microbial fertilizer, the yield of the American ginseng is increased by 30-35% effectively and diseases rate is reduced by more than 90%. The fertilizer, when being used for long time, can effectively improve soil. The fertilizer is long-acting in fertilizer efficiency, achieves the effects in the whole growing period of American ginseng, and also has the effect of improving the quality of the American ginseng.

A formulation for promoting sinus health includes at least one probiotic agent in combination with N-acetyl cysteine (NAC) and a bioflavonoid or derivative thereof.

The invention discloses a compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as a preparation method and application thereof, and belongs to the field of the compound probiotic agent. The compound probiotic agent is prepared by mixing and compounding a lactobacillus plantarum KT-Lp9 microbial agent, a lactobacillus casei zhang microbial agent, a lactobacillus plantarum P-8 microbial agent, a bifidobacterium animalis subsp. lactis V9 microbial agent and a dilution carrier, wherein the quantity of viable bacteria of the four kinds of microbial agents is more than or equal to 2*10<11>CFU / g. The compound probiotic agent with different contents of viable bacteria only can be applied to 96 dogs at different ages for 60 days, so that the immunity of the test dogs can be improved, and the incidence of diarrhea and the sickness degree are reduced. Main representations are that the feed intake and growth rate of the dogs are increased, leukocyte counts, lymphocytes counts and neutrophil counts in blood are controlled, and the contents of four immunologic factors including immune globulin G, interleukin 6, interferon alpha and tumor necrosis factor alpha in the blood can be increased; and meanwhile, the content of secretory immunoglobulin A (SigA) in excrement is increased.

The invention discloses a piglet creep feed as well as a preparation method and application thereof. The piglet creep feed comprises the following components: corns, extruded corns, extruded rice, spray-dried egg powder, extruded soybeans, soybean meal, fermented soybean meal, dried porcine soluble, fish soluble protein, an astragalus membranaceus polysaccharide extract, a herba houttuyniae extract, an acanthopanax senticosus extract, licorice powder, a probiotic agent, fish meal, whey powder, soybean oil, mountain flour, calcium hydrophosphate, a complex enzyme preparation, L-lysine, methionine, threonine, choline chloride, zinc oxide, an acidifier, ferrous glycine, a flavoring agent, a sweetening agent, an antioxidant, a mildew preventive and nucleus materials for weaned piglets; all the components are mixed uniformly to prepare the creep feed. The piglet creep feed provided by the invention can not only solve the problems about digestion coefficient and absorptivity of weaned piglets, but also improve the immunity of weaned piglets, promote gastrointestinal development and reduce diarrhea of piglets, can further fully replace a creep feed containing plasma proteins, therefore the piglet creep feed can serve as a high-quality piglet creep feed for application.

The invention discloses a chicken feed probiotic agent containing enterococcus faecalis and a preparation method of the chicken feed probiotic agent. The chicken feed probiotic agent is prepared by virtue of the steps of evenly mixing enterococcus faecalis HEW-A131 active bacterial sludge, bacillus subtilis active bacterial sludge, saccharomyces cerevisiae active bacterial sludge and a stabilization protective agent together in the mass ratio of (0.1-0.8): (1-2.5): (0.1-0.5): 1, and then pelletizing, drying, coating and the like; the probiotic agent is capable of improving the utilization efficiency of an animal feed and saving the cost, and also capable of greatly improving the steady state of the internal environment of the digestive tract in an animal body, promoting the absorption and utilization of nutrition and promoting the growth of the animal; as a result, the productivity, the immune performance and the reproductive performance of the animal are obviously improved, the production cost is reduced and the economic benefit is increased.

An exemplary embodiment providing one or more improvements includes feeding animals with probiotic microbes encapsulated in a mixture of xanthan gum and chitosan, or in gelatin, specifically Pediococcus acidilactici and Saccharomyces boulardii. Such encapsulation protects the viability of the probiotic microbes against unfavorable temperatures. An exemplary embodiment providing one or more improvements includes methods of using viable probiotics in therapy of birds and mammals infected with infectious diseases. Probiotics acted as adjuvants in stimulating antibody reaction and stimulated a cellular immunity response. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

The invention relates to an environmental protection technology for processing a threonine fermentation wastewater by using a biological agent. The process comprises the following steps: separating mycoprotein from threonine fermented liquid by virtue of a high-speed disc separator, recovering mycoprotein sediments, and collecting supernate; filtering the supernate by virtue of a film; recovering mycoprotein by virtue of the film, collecting filtrate, merging the mycoprotein, and adjusting the solid content to prepare a probiotic agent in a fermentation manner; concentrating, carrying out isopotential sedimentation on a production wastewater generated in the threonine extraction step; carrying out natural sedimentation and solid-liquid separation, discharging the supernate into a sewage treatment system; and adjusting the pH and adding a composite microbial agent to carry out advanced treatment, so as to discharge with standards. The process disclosed by the invention is low in wastewater discharge, economical and environmentally friendly, and has a wide application prospect.

The invention relates to fattening pig feeds and a preparation method thereof. The fattening pig feeds are prepared from the following components in parts by weight: 80 to 90 parts of basal feeds, 7 to 15 parts of a traditional Chinese medicament, 3 to 5 parts of a probiotic agent and 0.05 to 0.1 part of a vitamin complex. The probiotic agent is prepared by a method comprising the following steps: (1) preparing a medical stone extracting solution: weighing and crushing medical stones to 150 to 200 meshes, adding water according to a material-liquid ratio of 1 gram of medical stones to 100 to 150 milliliters of water, performing high-pressure cooking for 2 to 3h, and filtering to obtain filtrate and filter residues; (2) uniformly mixing 30 to 40 parts by weight of bran, 20 to 30 parts by weight of corn flour, 10 to 15 parts by weight of straw powder, 10 to 15 parts by weight of rice husks and 5 to 8 parts by weight of the filter residues obtained in step (1), regulating the water content to be 30 to 50 percent by virtue of the filtrate obtained in step (1), and sterilizing to obtain an activated culture medium; (3) inoculating probiotics to the activated culture medium according to an inoculation amount of 10 to 15 percent, and culturing for 2 to 3 days at 30 to 37 DEG C; (4) adding 5 to 10 parts by weight of xanthan gum into the culture medium in which the probiotics are cultured, uniformly mixing, then granulating and drying to obtain the probiotic agent. According to the fattening pig feeds, the feed conversion rate can be effectively increased.

The invention discloses a probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis, and its preparing method, and belongs to the technical field of feed microbes. The probiotic agent having the advantages of special sour fragrance, high number of contained live bacteria and improved degradation rate of proteins in the soybean meal, wheat bran and corn flour is prepared through the solid-state fermentation of soybean meal, wheat bran and corn flour by adopting Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9; and the method has the characteristics of low production cost and simple operation. The adoption of mixed bacterium segment solid state fermentation can utilize the mutualism synergism of strains, and can improve the yield and the thallus concentration; and the probiotic agent utilizing the growth, propagation and metabolism of the Lactobacillus plantarum HM-10 in the early stage of fermentation can provide abundant nutrients and necessary anaerobic environment for the propagation of the Bifidobacterium lactis V9, so it is helpful for the growth of the Bifidobacterium lactis V9, and the number of Lactobacillus plantarum HM-10 live bacteria is increased.

The invention belongs to the field of feed and in particular relates to a pig feed for the latter fattening period. The pig feed disclosed by the invention comprises corn flour, distillers' grains of Baijiu, wheat bran, soybean meal, peanut meal, peanut vine meal, sesame seed meal, rapeseed dregs, palm meal, rice bran, sweet potatoes, potatoes, sweet potato vine meal, brown seaweed, a complex enzyme preparation, a complex-probiotic agent, citric acid, sodium carboxymethyl cellulose, edible salt, fishbone dust, shell powder, calcium carbonate, lysine, methionine, ginger powder, premix, soybean dietary fiber flour, green feed and chitosan. The feed formula disclosed by the invention has the innovation that the green feed is added, so that the absorption and digestion ability of pigs in the latter fattening period is improved, the growth speed of the pigs is improved, and the disease resistance of the pigs in the latter fattening period is improved. In addition, a variety of feeds which are difficult to digest and absorb are fermented and transformed into components which are easy to digest and absorb, and the growth of the pigs in the latter fattening period is facilitated.

The invention discloses a preparation method of a composite probiotic agent for preventing and treating diabetes and controlling body weight, in particular to a composite probiotic agent formed by compounding probiotics, prebiotics and metformin and used for preventing and treating diabetes and controlling body weight and a preparation method of the composite probiotic agent. Beneficial bacteria such as clostridium butyricum, saccharomyces boulardii, lactobacillus reuteri, bifidobacterium infantis, bacillus licheniformis and the like, the prebiotics such as arabinose and xylooligosaccharide, and metformin are combined to avoid symptoms such as nausea, emesis, diarrhea, abdominal pain, constipation, abdominal distention, dyspepsia, heartburn and the irritable bowel syndrome which are caused by the metformin, the curative effect is good, glucose metabolism is accelerated by directly absorbing and utilizing glucose, and effects of controlling body weight and treating the diabetes are improved. In the aspect of improving adverse effects of the metformin drug to a gastrointestinal tract, the effect of the composite probiotic agent is higher than that of the metformin which is applied purely.

The invention relates to an aquatic probiotic agent. The aquatic probiotic agent comprises rhodopseudomonas palustris HEW-GJ106 which is collected in CGMCC (China General Microbiological Culture Collection Center) with the collection number of CGMCC NO.17021. The aquatic probiotic agent also comprises one or more of enterococcus faecalis, lactobacillus plantarum, pediococcus acidilactici, bacillussubtilis or saccharomyces cerevisiae. The invention also provides a preparation method and an application of the aquatic probiotic agent. The probiotic agent can improve the utilization efficiency ofaquatic animals, the cost is saved, the steady state of the internal environment of digestive tracts in animal bodies is greatly improved, nutrition absorption and utilization are promoted, animal growth is promoted, and the production performance, the immunity performance and the stress resistance of animals are notably improved.

Disclosed is a method of manipulating the rate of gastrointestinal transit in a mammalian subject. Also disclosed is the use, in the manufacture of a medicament for the treatment of constipation, of a selective inhibitor of methanogensis, a methanogen-displacing probiotic agent, or a prebiotic agent that inhibits the growth of methanogenic bacteria or promotes the growth of competing non-methanogenic intestinal flora. Alternatively, in accordance with the invention, is disclosed the use in the manufacture of a medicament for the treatment of diarrhea, of methane or a methane precursor, a methanogenic or other methane-enhancing probiotic agent, or a methanogenesis-enhancing prebiotic agent.

The invention discloses a cosmetic product. The plant fermentation composition with whitening and spot-fading functions is characterized by being prepared by taking sea buckthorn fruit oil, an aloe extracting solution, a radix salviae miltiorrhizae extracting solution and a radix morindae officinalis sugar chain solution as substrates and fermenting the substrates by using lactobacillus gasseri ATCC19992 and citrobacter flavus CGMCC.No 2998. In recent years, a large number of studies find out that after a specific strain is selected for fermenting the plant, the beautifying effect can be obviously improved; however, a research and development team of the company finds that the product is derived from pure natural plants, can achieve very remarkable whitening and spot-fading effects after being fermented by a probiotic agent, has excellent spot-fading and decolorizing effects, and particularly has very remarkable effects on chloasma and intractable pigmentation after being researched and practiced for many years.

Methods for determining the susceptibility or resistance of bacteria to antibiotic agents are provided. In one embodiment, the methods include culturing the bacteria in the presence or absence or theantimicrobial agent to generate a primary culture which is then cultured in the presence or absence of transforming phages. The recombinant phages are specific to the bacteria and comprise a heterologous marker (e.g., a nucleic acid that is expressible as a detectable product such as an RNA or a protein). The susceptibility or resistance of the bacteria to the antimicrobial agent may be determinedby assaying the culture for the presence or absence of the heterologous marker, wherein a reduction in the level or activity of the marker in the culture compared to the level or activity of the marker in a comparative culture indicates that the bacteria is sensitive to the antibiotic agent.

The present invention relates to a method for producing a probiotic agent of live Clostridium butyricum, belonging to the field of microbial technology and specially relates to a solid fermentation method for producing the probiotic agent of live Clostridium butyricum. The method comprises the steps of: employing the clostridium butyricum as a fermentation strain which is inoculated on an optimized solid fermentation medium, fermenting under conditions of isolation from air and a temperature of 35-42 DEG C for 48-72h, drawing off and determining a viable cell density, drying, crushing, and packaging to finished products. The method of the invention is advantageous in that: the production process is simple and stable, and easy for promotion; soybean meal and corn flour are used as main ingredients for the medium, greatly reducing the cost of production; the viable cell density is high, the maturation rate of spores is high, stable product quality; and the product quality is stable; and the fermentation product can be directly dried for use without purification, reducing costs and improving the product quality.

The invention relates to a bionic feed for breeding adult mandarin fish. The bionic feed is prepared from the following raw materials in parts by weight: 40-50% of enzymolysis fish soluble extracts, 10-15% of shrimp powder, 10-18% of fermented soybean meal, 2-5% of vital wheat gluten, 5-10% of fermented corn protein powder, 7-11% of high gluten flour, 1.5-2% of calcium dihydrogen phosphate, 1-2% of multiple vitamins, 1-2% of multiple minerals, 3-6% of refined fish oil, 2-4% of a composite phagostimulant and 1-2% of high-activity probiotic agents. The bionic feed for breeding adult mandarin fish disclosed by the invention wholly replaces forage fish. The feed has movement and mouth feel of living forage fish, and is extremely easy to ingest, digest and absorb by mandarin fish. When the bionic feed for mandarin fish is applied for replacing the forage fish, the breeding density and the yield can be increased, the cost of the feed can be reduced, the yield per unit is increased, the waterexchange amount is reduced, and economic benefits are increased.

The invention relates to a preparation method of a composite solid-state probiotic agent including phellinus linteus. The method comprises the following steps of inoculating phellinus linteus seed fluid to solid-state fermentation culture mediums, performing fermentation, and degrading cellulose and hemicellulose type substances for producing monosaccharide; and then sequentially adding after fermentation bacterial fluid for a plurality of times, performing fermentation to obtain fermentation products, and then drying the fermentation products so as to obtain the composite solid-state probiotic agent. According to the preparation method disclosed by the invention, stagewise solid-state fermentation is performed according to requirement differences of different probiotics for nutrient substances, so that the culture mediums can be efficiently decomposed; phellinus linteus grows through solid-state substrates to produce active polysaccharide, and besides, the cellulose and hemicellulose type substances are degraded to generate the monosaccharide; and the solid-state probiotic agent has special paste fragrance, contains fungus polysaccharide, is high in effective viable count, is high in the content of organic nitrogen, and has the efficacy of adjusting the flora in the intestinal tracts of animals, enhancing immunity, increasing the utilization rate of the solid-state substrates, reducing the fetor of excrement of livestock and improving environment.

The present invention discloses a Bacillus licheniformis-Bacillus subtilis-Lactobacillus casei composite probiotic preparation and a preparation method thereof, and belongs to the technical field of feed microorganisms, wherein soybean meal, wheat bran and corn powder are adopted as matrixes, and Bacillus licheniformis-Bacillus subtilis-Lactobacillus casei are adopted to carry out solid state fermentation to obtain the probiotic preparation. According to the present invention, the preparation method has characteristics of low production cost, economic benefit increase and simple operation, and can be widely used for the probiotic solid state fermentation technology in the microecology field; the bacterial mixing solid state fermentation is adopted, and the mutual-benefit symbiotic synergism effect among the strains is adopted, such that the yield and the bacterial concentration can be increased, the number of live bacteria in the obtained fermented product is high, and the proteins in the soybean meal and the corn powder are easily decomposed into small peptides and free amino acids so as to be easily digested and absorbed by intestine and stomach; and when the product is added to a feed, the feed utilization efficiency can be effectively increased by 13-25% when the product is fed to milk cow.

The present invention relates to a method for producing a probiotic agent of live Lactobacillus bulgaricus, belonging to the field of microbial technology and specially relates to a liquid anaerobic fermentation method for producing the probiotic agent of live Lactobacillus bulgaricus. The method comprises the steps of: employing the Lactobacillus bulgaricus as a fermentation strain which is inoculated on an optimized liquid fermentation medium, fermenting at a temperature of 37-42 DEG C for 12-14h, drawing off and determining the number of viable cells, adsorbing fermentation broth by using vegetable starch, drying, crushing, and packaging to prepare the probiotic agent of live Lactobacillus bulgaricus with stable viability. The method of the invention is advantageous in that: high density fermentation of the Lactobacillus bulgaricus can be completed by using an ordinary fermentation tank, with simple operation, stable effect and low energy consumption; and centrifuges, freeze-drying machines, etc. are not required, thus reducing production costs and improving production efficiency.

The invention discloses a composite probiotic agent for improving the intestinal functions of piglets, and a preparation method thereof. The composite probiotic agent comprises a mixed bacterial liquid and a composite carrier, the mixed bacterial liquid is a Bacillus velezensis, Enterococcus, Bacillus subtilis and Enterobacter cloacae mixed bacterial liquid, and the composite carrier is a mixtureof zeolite powder and whey powder. The preparation method comprises the following steps: mixing the mixed bacterial liquid with expanded corn flour, and then mixing the obtained mixture with the composite carrier. Compared with composite probiotic agents in the prior art, the composite probiotic agent in the invention is especially suitable for piglets having incompletely developed intestinal functions, and can aid digestion, soften night soil and improve the immunity and the growth performance of the piglets when applied to feed additives for the piglets.

The invention relates to complex probiotic for treating and preventing allergic diseases as well as a preparation method and a preparation of the complex probiotic. The complex probiotic is characterized by being prepared from strain composition shown in the description in percentage by weight.

The invention discloses a synbiotic feed additive for growing and fattening of pigs. The synbiotic feed additive is prepared from composite probiotic agents, maltodextrin, isomaltooligosacharide, glucose, citric acid, composite vitamins, composite trace elements, charcoal, attapulgite, leguminosae, dioscorea opposita, poria cocos, atractylodes macrocephala rhizomes, dried unripe fruits of citrus aurantium, common vladimiria roots, Chinese thorowax, dried ripe fruits of crataegus pinnatifida, oblongleaf kadsura stems or roots and bidens pilosa. The invention further provides an application method of the additive. During the growth and fattening period of pigs, the synbiotic feed additive for growing and fattening of pigs is added to a feed according to the addition quantity of the synbioticfeed additive to the body weight of fattening pigs being 0.1-5% for feeding. Through adoption of the synbiotic feed additive for growing and fattening pigs and the application method disclosed by theinvention, the weight increment speed of the body weight of the pigs can be increased, the lean meat proportion is increased, the nutrients are comprehensive, the feed feeding quantity can be reduced, the utilization rate of the feed can be increased, the growth speed of the pigs is high, the meat quality is bright red, tender in nature and good in mouth feel, the economic benefits are increased,and the synbiotic feed additive has good application prospects.