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Composite probiotic agent for improving intestinal functions of piglets, and preparation method thereof

A technology of compound probiotics and intestinal function, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as low product quality, threats to human health, and many livestock and poultry diseases, so as to improve the intestinal tract function, enhancing immune performance and growth performance

Active Publication Date: 2018-03-02
佛山市顺德区兄弟德力饲料实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has always been a huge problem that has been plaguing farmers, such as many diseases in livestock and poultry, and low product quality; at the same time, food safety problems caused by drug residues in meat, eggs, milk and food are threatening human health

Method used

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  • Composite probiotic agent for improving intestinal functions of piglets, and preparation method thereof
  • Composite probiotic agent for improving intestinal functions of piglets, and preparation method thereof
  • Composite probiotic agent for improving intestinal functions of piglets, and preparation method thereof

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Embodiment 1

[0021] 1. Materials

[0022] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0023] 2. Method

[0024] 2.1 Preparation of mixed bacterial solution

[0025] Weigh 50 grams of LB medium, dissolve it in 2000m L of tap water, mix well, divide into four parts, sterilize with high pressure steam at 121℃ for 20 minutes, and inoculate 5m L of Bacillus Veles, Enterococcus, Bacillus subtilis and Enterococcus cloacae Bacillus seed bacterial liquid, under the condition of 37 ℃, 120r / min, carry out expansion culture, adjust to the concentration of Bacillus velei, Enterococcus, Bacillus subtilis and Enterobacter cloacae to be 6.5-8.3×10 13 CFU / mL, 3.5-6.6×10 12 CFU / mL, 5.1-7.6×10 12 CFU / mL and 5.5-6.8×10 12 CFU / ml.

[0026] The formula of LB medium is: tryptone 20g, yeast extract 10g, NaCl 20g.

[0027]...

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Abstract

The invention discloses a composite probiotic agent for improving the intestinal functions of piglets, and a preparation method thereof. The composite probiotic agent comprises a mixed bacterial liquid and a composite carrier, the mixed bacterial liquid is a Bacillus velezensis, Enterococcus, Bacillus subtilis and Enterobacter cloacae mixed bacterial liquid, and the composite carrier is a mixtureof zeolite powder and whey powder. The preparation method comprises the following steps: mixing the mixed bacterial liquid with expanded corn flour, and then mixing the obtained mixture with the composite carrier. Compared with composite probiotic agents in the prior art, the composite probiotic agent in the invention is especially suitable for piglets having incompletely developed intestinal functions, and can aid digestion, soften night soil and improve the immunity and the growth performance of the piglets when applied to feed additives for the piglets.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a compound probiotic agent for improving intestinal function of piglets and a preparation method thereof. Background technique [0002] In recent years, the level of my country's animal husbandry industry has grown rapidly, and livestock breeding has played an important supporting role in my country's economic industry and its proportion in the national economy is increasing day by day. However, there has always been a huge problem that has been plaguing farmers, such as many diseases in livestock and poultry, and low product quality. At the same time, food safety problems caused by drug residues in meat, eggs, milk, and food are threatening human health. As food safety issues become increasingly prominent, livestock, poultry, meat, eggs, and milk products that do not contain additives such as antibiotics and hormones have gradually replaced the previous products and become people's pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/07C12R1/46C12R1/01C12R1/125
Inventor 王在贵叶淼丁仁源孙玲红杨濡
Owner 佛山市顺德区兄弟德力饲料实业有限公司
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