215 results about "Methanogenesis" patented technology

This invention generally relates to natural gas and methylotrophic energy generation, bio-generated fuels and microbiology. In alternative embodiments, the invention provides nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis, or “methylotrophic” conversion. Additionally, the invention provides methods to develop nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis in a given reservoir. The invention also provides methods for the evaluation of potentially damaging biomass formation and scale precipitation resulting from the addition of nutrient amendments. In another embodiment, the invention provides methods for simulating biogas in sub-surface conditions using a computational model.

Processes for biogenic production of a hydrogen-carbon-containing fluid from a hydrocarbon containing formation are described. The processes may include providing in the formation an anaerobic microorganism consortium containing one or more enzymes to activate a starting hydrocarbon by an addition of a chemical group to the hydrocarbon. The processes may further include converting the activated hydrocarbon into the hydrogen-carbon-containing fluid through one or more intermediate hydrocarbons, and recovering the hydrogen-carbon-containing fluid from the formation.

The invention provides a method for producing biogas through high-solid two-phase three-stage anaerobic digestion by using perishable organic wastes, which comprises the following steps: the perishable organic wastes are hydrolyzed in a hydrolysis acidogenic reactor, and a mixture obtained after hydrolysis is acidified to generate a large number of organic acid products with low molecular weight; the bottom of the hydrolysis acidogenic reactor is communicated with the bottom of a methanogenic reactor through a circulation pump and a pipeline; the top of the methanogenic reactor is communicated with the top of the hydrolysis acidogenic reactor; and the acetic acid producing reaction is carried out in the methanogenic reactor and the methane producing reaction is continuously carried out. The invention can separate the hydrolysis acidogenic process from the acetic acid and methane producing process in the anaerobic digestion process so as to prevent organic acids generated by the perishable organic wastes from inhibiting methanogenesis. The device has simple processing procedure, low cost, easy maintenance and stable and reliable performance, is suitable for anaerobic digestion treatment of various perishable organic wastes, and has highly efficient and stable biogas production capacity of the perishable organic wastes through the anaerobic digestion.

The present invention relates to the field of degradation with hyperthermophilic organisms, and in particular to the use of hyperthermophilic degradation to produce heat and energy rich components including hydrogen and ethanol from a biomass. In some embodiments, a biomass is fermented in the presence of hyperthermophilic organisms to produce heat. The heat is used to heat a liquid which is used directly in a heat pump or radiant heat or to produce electricity or drive a steam turbine. In some embodiments, acetate is utilized as a substrate to produce energy by methanogenesis.

The invention discloses a device and a method for treating low-carbon nitrogen sewage in a synchronous anaerobic ammonia oxidation and anaerobic methane oxidation mode, belonging to the technical field of sewage treatment. The device mainly comprises a sewage anaerobic methanogenesis reactor, a sludge anaerobic digestion tank, a synchronous anaerobic ammonia oxidation and anaerobic methane oxidation reactor, a partial nitrification reactor, a water intake pool and a water outtake pool, wherein anaerobic digestion sludge is respectively inoculated to the sewage anaerobic methanogenesis reactor and the sludge anaerobic digestion tank; and cultured and domesticated anaerobic ammonia oxidation and denitrification type methane anaerobic oxidized bacterium symbiotic bacteria flora sludge is inoculated to the synchronous anaerobic ammonia oxidation and anaerobic methane oxidation reactor, and cultured and domesticated partial nitrification sludge is inoculated to the partial nitrification reactor, so as to carry out sewage treatment. By utilizing the device and the method, the problems that the organic carbon source is insufficient and the ratio of C to N is low in sewage denitrification treatment are solved.

The invention discloses a rapid culture method of simultaneous desulfidation and denitrogenation granular sludge. The method is characterized in that an upflow anaerobic sludge bed reactor is adopted, anaerobic ammoxidation granular sludge and methanogenesis granular sludge are adopted as inoculation sludge, model wastewater containing ammonia nitrogen and nitrite nitrogen is adopted as inlet water, and the method comprises the following steps: adding sulfide into the model wastewater, adding inorganic salts and trace elements for maintaining the growth of microbes, gradually improving the concentrations of matrixes in the inlet water and / or shortening the hydraulic retention time in order to adjust the running of the reactor, and culturing under anaerobic and shady conditions at 30-37DEG C under the pH value of the inlet water of 7.5-8.0. The sludge obtained in the invention has the advantages of obvious granularity, strong impact resistance, high processing efficiency, saving of the occupied are through cooperating with a UASB reactor, simplification of the desulfidation and denitrogenation process, and low investment and running cost, and is suitable for treating wastewater containing ammonia nitrogen, nitrite nitrogen and sulfides of the light industry, the pharmacy industry, the oil refining industry and the leather making industry.

The invention relates to a method for utilizing nano ferroferric oxide for improving the activity of anaerobic digestion methanogens and methanogenesis efficiency. The method aims at solving the problems that in the prior art, the addition concentration of Fe2+ / Fe3+ is hard to control, negative ion inhibition is caused, bioavailability is low, the chemical property is unstable, and cost is high. The method comprises the steps that short chain fatty acid salt is adopted as an electron donor, amine salt is adopted as a nitrogen source, phosphate is adopted as a phosphorus source, system basicity is maintained, and under the culture condition of continuous flow anaerobic fermentation, anaerobic mixed granule sludge is separated, and mixed seed sludge is obtained; the short chain fatty acid salt is adopted as the electron donor, the amine salt is adopted as the nitrogen source, the phosphate is adopted as the phosphorus source, the system basicity is maintained, the nano ferroferric oxide is adopted as an iron ion donor, the mixed seed sludge is adopted as inoculation sludge, and culturing is carried out under the static anaerobic fermentation condition. The method is used for improving the activity of the anaerobic digestion methanogens and the methanogenesis efficiency.

This invention generally relates to natural gas and methylotrophic energy generation, bio-generated fuels and microbiology. In alternative embodiments, the invention provides nutrient amendments and microbial compositions, e.g., consortia, that are both specifically optimized to stimulate methanogenesis, or for “methylotrophic” or other conversions. In alternative embodiments, the invention provides methods to develop nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis in a given reservoir. The invention also provides methods for the evaluation of potentially damaging biomass formation and scale precipitation resulting from the addition of nutrient amendments. In other embodiments, the invention provides methods for simulating biogas in sub-surface conditions using a computational model.

Disclosed is a method of manipulating the rate of gastrointestinal transit in a mammalian subject Also disclosed is the use, in the manufacture of a medicamens for the treatment of constipation, of a selective inhibitor of methanogensis, a methanogen-displacing probiotic agent, or a prebiotic agent that inhibits the growth of methanogenic bacteria or promotes the growth of competing non-methanogenic intestinal flora. Alternatively, in accordance with the invention, is disclosed the use in the manufacture of a medicament for the treatment of diarrhea, of methane, or a methane precursor, a methanogenic or other methane-enhancing probiotic agent, or a methanogenesis-enhancing prebiotic agent.

The invention relates to a sludge treatment method and process of synchronously generating electricity and methane by using excess sludge by a microbial fuel cell. The process mainly comprises anaerobic digestion of sludge and microbial electrogenesis. The operating method comprises the following steps: first, concentrating the excess sludge; mixing with anaerobic digested sludge; adjusting the pH value to an appropriate value; then, adding into an anode chamber of the microbial fuel cell; and controlling mixing and temperature of the mixed sludge in the anode chamber for anaerobic digestion and electrogenesis. The method provided by the invention is simple in process, simple and convenient to operate and control and low in operating cost and energy consumption and can generate electricity and methane by sludge. The output voltage of sludge electrogenesis is improved by 0.02-0.10V compared with that under the condition of just electrogenesis, the electrogenesis power is improved by 10-20%, and the methanogenesis is not affected and even improved by 5-10%. The residual organic matters in sludge after treatment can be reduced by 20-30% compared with those after conventional anaerobic digestion. Therefore, the sludge resource utilization and reduction efficiency are improved.

The invention belongs to the technical field of environmental protection and relates to a method for improving the methanogenesis of residual sludge by heat and alkali combined pretreatment. According to the method, after the residual sludge is thermally pretreated, the dissolving in water of organic matters such as protein and polysaccharide in the residual sludge can be improved significantly so that abundant water-soluble organic matrixes can be provided for the fermentation of the sludge and the generation of acids; and in addition, after the residual sludge is pretreated under the initial alkaline condition, the dissolving and the dehydrating of the sludge can be promoted further and short chain fatty acids can be produced continuously. Therefore, the residual sludge dehydrating efficiency and the acid generating efficiency can be improved greatly by the combination of thermal pretreatment and the initial alkaline condition so that the yield of methane can be increased to the maximum extent.

The invention provides a method for realizing methanogenesis with surplus sludge by utilizing a microbial electrolytic tank, relates to a method for methanogenesis, and aims at solving the problems that a conventional fermentation method cannot completely utilize surplus sludge and is longer in time. The method comprises the following steps: firstly, taking surplus sludge as a substrate for acclimatization of anode functional microorganism and starting a microbial electrolytic tank reactor; and secondly, under the condition that the electrolytic tank is stable, taking the surplus sludge as the substrate for methanogenesis. The method completely utilizes the surplus sludge, and is short in reaction time; the methanogenesis rate is 0.10-0.25m<3>H2 / m<3>.d; and the protein removal rate is as high as 38%-58%, and the COD (chemical oxygen demand) removal rate is up to 38%-55%. Due to the higher removal rates of the protein and COD, the method for treating surplus sludge through the microbial electrolytic tank technology and recycling the energy product methane with higher economic value has the characteristic of combining productivity with reduction of environmental pollution.

The invention relates to a printing and dyeing wastewater pretreatment method based on an anoxic zone phase separation technology. According to the printing and dyeing wastewater pretreatment method, an anoxic zone is divided into a phase I and a phase II, namely a phase for hydrogen production and acid production through fermentation and a methanogenesis phase; an electron donor is provided by organic matters in the wastewater, and dye is effectively decolored in the phase I by using a sulfate reducing process and the reducing capacity of reduzate, namely the sulfide, therefore the biodegradability of the wastewater is improved; and the organic matters can be efficiently removed in the phase II. As an anoxic phase transformation technology in the printing and dyeing wastewater treatment course, the printing and dyeing wastewater pretreatment method is used for providing a two-phase anoxic system and carrying out pretreatment on the printing and dyeing wastewater; the chromaticity and the COD (Chemical Oxygen Demand) removal rates can be effectively increased, the biodegradability of the wastewater can be greatly improved, and the defects that a traditional anoxic process is instable in treatment effect, large in sludge yield and the like are overcome. The printing and dyeing wastewater pretreatment method can be used for effectively removing chromaticity and COD in the printing and dyeing wastewater, the removal rates of the chromaticity and the COD respectively reach more than 95 percent and 60 percent, and the organic loading to subsequent procedures is greatly reduced.

The invention relates to a method for strengthening anaerobic digestion of sludge by utilizing kitchen waste pre-alcoholization. The method mainly comprises the following steps: (1) performing ethanolpre-fermentation on kitchen wastes, namely feeding uniformly crushed kitchen wastes into an alcohol production phase reactor, adding yeast or adjusting the pH value and the like to make the kitchen wastes subjected to rapid and efficient anaerobic fermentation to produce ethanol, and respectively collecting fermentation liquor and fermentation residues; (2) pretreating sludge: mixing the sludge with kitchen waste fermentation residues, and then carrying out pretreatment; and (3) carrying out anaerobic digestion to produce methane: mixing the pretreated product with the kitchen waste fermentation liquor, and carrying out anaerobic digestion in a methane production phase reactor to produce methane. The invention provides a novel thought of synergistic anaerobic digestion, and ethanol in thekitchen waste fermentation liquor can enrich electroactive microorganisms in a methanogenesis phase, so that the interspecific electron transfer rate of the microorganisms is increased, and the methanogenesis efficiency is improved. According to the invention, through a multiphase reaction, resource recovery and biogas upgrading are enhanced while sludge and kitchen waste are effectively treated.

The present invention concerns the reduction of gastro-intestinal methanogenesis in ruminants with the aid of agents that compete for the hydrogen atoms required by methanogens during normal fermentation of ingested feed. The invention in one aspect resides in the findings that both nitrate reductive pathways as well as sulphate reductive pathways outcompete gastro-intestinal methanogenesis in ruminants and, that the methanogenesis reducing effects of nitrate and sulphate are completely additive. At the same time the combined administration of nitrate and sulphate was found to be fully effective to avoid or mitigate the potential problems of nitrite intoxication normally encountered when using nitrate alone, which effect is further enhanced, where necessary, by the addition of a nitrite reducing probiotic micoroorganism. Hence, products are provided comprising high amounts of a combination of a nitrate compound and a sulphate compound and optionally a nitrite reducing probiotic microorganism, as well as methods of reducing gastro-intestinal methanogenesis in ruminants using such compositions.

The invention discloses a biological enzyme treating agent of industrial sewage. The biological enzyme treating agent is characterized by comprising, by weight, 10 parts to 18 parts of oxidation-reduction enzymes, 10 parts to 15 parts of cellulose, 5 parts to 8 parts of protease, 5 parts to 8 parts of laccase, 6 parts to 8 parts of methanogenesis brevibacterium, 6 parts to 8 parts of nitrococcus, 0.5 parts to 1.5 parts of bamboo juice, and the balance water. According to the biological agents conduct disposing on sewage of printing and dyeing mills and similar industries through a biological catalysis technology which has a biological degradation function, foul smell can be dispelled greatly so as to degrade liquid pollutants rapidly and reduce the size of solid substances, polluted substances can be purified rapidly, treatment effect is good, operating cost of sewage treatment can be reduced greatly, the purified water is stable in water quality, the biological enzyme treating agent of the industrial sewage is energy-saving, efficient and capable of treating pollution, and has notable economical benefits and environmental benefits.

The invention discloses a compound quick-action inhibitor against generation of methane in the technical field of greenhouse gas emission reduction. The preparation method comprises: 1) mixing nitroethane, 2-nitroethylalcohol and 2-nitryl-1-propyl alcohol by weight percent of 0-5:0-6:0-7 (the weight percents can not be 0 simultaneously) to formulate solution A; and 2) mixing pyromellitic formic diimide and 2-bromoethane sodium sulfonate by weight percent of 0-3:0-2 (the weight percents can not be 0 simultaneously) to formulate powder B. The compound quick-action inhibitor has very obvious effect in inhibiting methane, is especially applicable to inhibiting the action of generating methane in rumen, and is beneficial to protecting the environment and improving the utilization rate of feed energy of ruminants. The preparation method is simple, the operability is strong, and the inhibitor is applicable to livestock and poultry breeding feed processing, livestock and poultry manure excrement biogas treatment and municipal domestic waste biogas treatment, and has the efficiency up to over 90 percent in inhibiting the action of generating methane by microorganism, as well as high practical value.

The invention relates to a combined device for synchronous denitrification and methanogenesis as well as autotrophic nitrogen removal of landfill leachate and a method thereof. The device is provided with an integrated water tank, a synchronous denitrification and methanogenesis reactor, an A / O shortcut nitrification reactor, a settling tank, and an anaerobic ammoxidation reactor. A mixed solution of stock leachate and an effluent reflux liquid of the anaerobic ammoxidation reactor firstly flows into the synchronous denitrification and methanogenesis reactor, the effluent of which then flows into the A / O reactor for shortcut nitrification and denitrification, and the effluent of the settling tank flows into the anaerobic ammoxidation reactor. The outlet pipe of the synchronous denitrification and methanogenesis reactor is connected to the anaerobic ammoxidation reactor. The method consists of the steps of: starting the synchronous denitrification and methanogenesis reactor, starting the A / O shortcut nitrification reactor, starting the anaerobic ammoxidation reactor, and bringing the three reactors into series operation. The device of the invention is suitable for removal of COD (chemical oxygen demand) and nitrogen from early and medium term landfill leachate of landfills, and has obvious advantages of energy saving and consumption reducing.

The invention discloses a method for increasing methane yield by preprocessing rice straw through rumen microorganisms, and belongs to the fields of biotechnologies and renewable energy. The method includes the steps of filtering fresh rumen fluid, storing filtrate at a certain temperature, mixing rice straw powder with a nutrient solution according to a certain proportion, inoculating rumen fluid of a certain proportion, placing the mixed fluid in a constant-temperature oscillation water bath kettle, conducting anaerobic cultivation at a certain temperature and a certain rotating speed, conducting rumen microorganism preprocessing, inoculating a certain proportion of anaerobic digestion inoculated sludge containing methanogens in the fermentation liquid after preprocessing ends, and conducting anaerobic digestion methanogenesis at a certain anaerobic digestion temperature and within the initial pH range. The rice straw biomass material is preprocessed through rumen microorganisms, hydrolytic acidification of the raw material is promoted, the high methane yield is obtained, and the reaction time is shortened; meanwhile, pressure for processing wastewater of slaughter houses on sewage treatment plants is relieved. Compared with the contrast group (not preprocessed), the methane yield is increased by 55.4-87.3%, the anaerobic digestion time is shortened by 3-10 days, the degradation rate of cellulose is increased by 20.3-33.6%, and the degradation rate of semi-cellulose is increased by 15.6-25.7%.

The invention discloses an efficient two-phase anaerobic fermentation device. The efficient two-phase anaerobic fermentation device comprises a hydrolysis acid production reactor, a high-temperature hydrolysis device and at least one methanogenesis reactor, wherein a bottom percolate storage chamber of the hydrolysis acid production reactor is connected with the bottom of the methanogenesis reactor; the lower part of the hydrolysis acid production reactor is provided with a cavity stainless cone-shaped bracket with holes; the bottom of the cone-shaped bracket is connected with the high-temperature hydrolysis device; the methanogenesis reactor is provided with an upper discharge hole and a lower discharge hole; the upper discharge hole and a feed inlet form a self-circulation pipeline; the lower discharge hole of the hydrolysis acid production reactor is connected with a rotary sprayer; the high-temperature hydrolysis device is used for receiving large particle solid separated by the cone-shaped bracket; and solid-liquid separation is carried out by a biogas residue press filter at the outlet. The device is simple in manufacturing procedure, reasonable in structure, easy in maintenance, high in fermentation efficiency and stable and reliable in performance, is suitable for anaerobic digestion of organic wastes, and meets the large-scale production requirement.

The invention belongs to the field of biomass energy sources and relates to a method for improving anaerobic methanogenesis efficiency by using nano magnetite. The method takes a short-chain fatty acid as an electron donor and the nano magnetite is used as an electron carrier; under an anaerobic fermentation cultivation condition, the short-chain fatty acid is degraded so as to improve syntrophic oxidization methanogenesis efficiency of the system. With the adoption of the method, the energy source utilization efficiency of the short-chain fatty acid can be obviously improved. The method has important meanings on the improvement of the operation efficiency of a methanogenesis reactor and also has good economic benefits.

The present invention concerns the reduction of gastro-intestinal methanogenesis in ruminants with the aid of agents that compete for the hydrogen atoms required by methanogens during normal fermentation of ingested feed. The invention in one aspect resides in the findings that both nitrate reductive pathways as well as sulphate reductive pathways outcompete gastro-intestinal methanogenesis in ruminants and, that the methanogenesis reducing effects of nitrate and sulphate are completely additive. At the same time the combined administration of nitrate and sulphate was found to be fully effective to avoid or mitigate the potential problems of nitrite intoxication normally encountered when using nitrate alone, which effect is further enhanced, where necessary, by the addition of a nitrite reducing probiotic micoroorganism. Hence, products are provided comprising high amounts of a combination of a nitrate compound and a sulphate compound and optionally a nitrite reducing probiotic microorganism, as well as methods of reducing gastro-intestinal methanogenesis in ruminants using such compositions.

The invention relates to a method for pretreating landfill leachate by a quasi aerobiotic landfilling body. The method for pretreating comprises the steps: constructing an urban domestic garbage quasi aerobiotic landfilling body; designing a proper reflux process to perform home position treatment on leachate by using a unique aerobiotic-facultative anaerobic-anaerobic environment of the quasi aerobiotic landfilling body. The method has the advantages that concentration of BOD5, CODCr and NH4<+>-N of the leachate is effectively reduced, and a stabilizing process of a garbage heap is accelerated. A specific process comprises the steps: the leachate flows into the landfilling body by a pump by a collection system, in an aerobic zone, ammonia is oxidated to NO3<-> under the action of autotrophic nitrobacteria, in an anoxic zone, NO3<-> is reduced to N2 by using denitrifying bacteria with organic matter as an electron carrier, N2 is released from a liquid phase and conversion and removal of NH4<+>- are realized, and degradation methanogenesis of organic matter is realized. The method can solve the prominent problem of overhigh ammonia nitrogen concentration in the landfill leachate and effectively reduces concentration of BOD5 and CODCr in the leachate.

The invention relates to a device and a method for combined treatment of soybean protein wastewater by anaerobic methanogenesis and anaerobic ammonia oxidation and belongs to the technical field of sewage treatment. The device is provided with a water inlet tank, the water inlet tank is communicated with an anaerobic SBR reactor by virtue of a first water inlet pump and a pipeline, the anaerobic SBR reactor is provided with a reaction zone and a top zone, the bottom of the reaction zone is provided with a microporous aeration head communicated with an air compressor, the upper part of the reaction zone is the top zone, a first overflow weir is arranged between the reaction zone and the top zone, the outside of the first overflow weir is communicated with a water outlet pipe and a gas outlet and a gas backflow pipeline are formed in the top of the reaction zone. The method comprises the following steps of 1) inoculating sludge; 2) carrying out start-up phase; and 3) continuously operating. The device and method disclosed by the invention are suitable for treating high-COD and high-ammonia-nitrogen wastewater generated in the production process of soybean protein and are also applicable to the denitrification treatment of high-ammonia-nitrogen industrial wastewater. According to the device and method, process is perfect, operating cost is low, sewage treatment effect is good, and efficiency is high and control is flexible.

The invention discloses a method for improving anaerobic digestion performance of excess activated sludge. The method comprises the steps: adding inoculated sludge and excess activated sludge into a reactor according to a volume ratio of 1 to 3, and adding distilled water into the reactor for dilution until the total solid concentration of the mixed sludge is 15.00g / L, the total volatile solid concentration is 9.54g / L and the total chemical oxygen demand is 1383gmg / L; adjusting the pH value of the mixed sludge to be 7.0+ / -0.2; then adding 1-30g / L rusted waste scrap iron and 1-5g / L magnesium chloride, and introducing N2; finally placing the reactor into a water bath constant-temperature oscillator, and continuing reaction for 30 days, wherein the revolving speed is 150rmp, the reaction temperature is 35 DEG C. According to the method, the rusted waste scrap iron and the magnesium chloride are added into the mixed sludge to enhance the anaerobic digestion methanogenesis performance of the excess activated sludge and improve the recovery rate of a phosphorus element.

The invention discloses a method for methanogenesis through anaerobic sludge digestion fortified by zero-valent nano-iron and belongs to the technical field of solid waste resource recovery. Firstly, pH of anaerobic digestion sludge is regulated to be 6.8-7.2; the sludge is placed in an anaerobic reaction flask under the protection of N2, N2 is introduced till ORP is smaller than -300 mV, zero-valent nano-iron is added at the same time, the concentration of zero-valent nano-iron is made to be 100-1000 mg / L, the anaerobic reaction flask is placed in a shaking table for anaerobic digestion, the rotating speed of the shaking table is set to be 150-180 rpm, anaerobic digestion reaction lasts 15-20 days, the concentration of methane in the gas phase is measured along the way with the gas chromatographic method, methane gas output is measured with the vapor equilibration method, and then the volume of methane is obtained through calculation. According to the method, input of extract energy is not needed, and use amount is smaller and cost performance ratio is higher compared with ordinary iron powder. The method is suitable for anaerobic digestion sludge and excess sludge.

The present invention refers to a method for reducing methane production in ruminants comprising administering to said ruminant a feed composition containing a flavanone glycoside.

The invention relates to a method for strengthening the autotrophic denitrification effect of urban sewage by adopting an oxidation ditch and belongs to the field of sewage treatment. The method comprises the following steps of: feeding the urban sewage into an aerobic zone of a high-load activated-sludge reactor so as to be subjected to anaerobic phosphorus release, feeding the urban sewage into an aerobic zone, adsorbing organic matters in the urban sewage onto activated sludge under the adsorbing action of the activated sludge, removing the phosphorus in the urban sewage by the way of aerobic phosphorus adsorbing, feeding the urban sewage in which the organic matters and the phosphorus are removed into the oxidation ditch, carrying out short-time nitrification and anaerobic ammonium oxidation to the urban sewage in a hypoxic zone, carrying out anaerobic ammonium oxidation to the urban sewage in an anoxic zone, and carrying out autotrophic nitrogen removal to the urban sewage by carrying out short-time nitrification and short-time anaerobic ammonium oxidation in the hypoxic zone and the anoxic zone repeatedly. By utilizing the method, the organic matters in the sewage can be enriched into the sludge in the process of removing the phosphorus biologically, the sludge can be used for the anaerobic methanogenesis, and the energy in the sludge can be recovered. In addition, the autotrophic nitrogen removal is realized by carrying out nitrification and anaerobic ammonium oxidation in the hypoxic zone and the anoxic zone repeatedly, and the energy consumed for treating the sewage can be reduced greatly.