A method suitable for mass production of various orchid medicinal plants
A batch technology of medicinal plants, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of different tissue culture propagation methods, increased pollution probability, uneven seedlings, etc., and achieve high maintenance Consistency, improved cultivation efficiency, and more effective seedlings
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Embodiment 1
[0081] Embodiment 1, the method that is suitable for the batch production of various orchid medicinal plants
[0082] 1. Culture medium preparation and sterilization
[0083] The first-generation subculture medium is used for the propagation of basic seedlings: MS improvement (the content of other elements in MS remains unchanged, and the content of organic components is 1.2 times) + 6-BA2.5mg / L + IAA0.5mg / L + NAA0.8mg / L + coconut milk 20mg / L;
[0084] Elongation and rooting medium for mass production: MS+paclobutrazol 2.0mg / L+chlormequat 2.0mg / L+GA 3 0.5mg / L;
[0085] The above medium was sterilized at 121°C for 20 minutes.
[0086] 2. Efficient basic seedling propagation
[0087] 1. Select the old perennial stems of Dendrobium officinale and Dendrobium Huoshanense and cut them off, disinfect the surface with 70% alcohol, place them in a culture dish with 3-4 layers of filter paper, observe the humidity of the filter paper, spray sterile water to moisturize, and promote t...
Embodiment 2
[0098] Embodiment 2, tissue culture rapid propagation
[0099] 1. Culture medium preparation and sterilization
[0100] First-generation subculture medium: MS improvement (the content of other MS elements remains unchanged, and the content of organic components is 1.2 times) + 6-BA3.0mg / L + IAA0.6mg / L + NAA1.0mg / L + coconut milk 40mg / L;
[0101] Elongation and rooting medium: MS+paclobutrazol 1.0mg / L+chlormequat 1.0mg / L+GA 3 1.0mg / L.
[0102] The above medium was sterilized at 121°C for 20 minutes.
[0103] Get dendrobium axillary buds, clematis clematis stem segments and bletilla striata pseudobulbs, the basic operation method is consistent with embodiment 1; the effect is not much different from embodiment 1.
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