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High-throughput breeding method for breadfruit seedlings

A high-throughput, breadfruit technology, applied in horticultural methods, botanical equipment and methods, vegetative propagation, etc., can solve the problems of long cycle, slow growth, small quantity, etc., and achieve the effect of high pollution rate

Active Publication Date: 2021-06-18
SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tracing it to its cause, the lack of good seedlings is an important factor restricting its industrial development, and the known good seedlings of breadfruit seedless breed rely on root tillers to reproduce naturally or stab the surface root system to produce root tillers, which grow slowly and have a long cycle. small and uncertain

Method used

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  • High-throughput breeding method for breadfruit seedlings
  • High-throughput breeding method for breadfruit seedlings
  • High-throughput breeding method for breadfruit seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: method of the present invention

[0037] Cut the root system of the collected breadfruit close to the surface, 1.5 cm to 6 cm thick, into 15 cm to 30 cm long, cut the upper part of the root segment flat, keep the incision flat when trimming the cut, and keep the root segment intact without cracking. The mouth is cut obliquely. The surface can be covered with 1 cm thick fine coconut peat, and the seedbed is sprayed once with 500 times of carbendazim. The upper 2 to 3 cm of the roots are exposed to the air, and a plastic film shed with a height of 50 to 80 cm is built on the seedbed. And set a shading net on the top to cover the top, water regularly to keep the humidity of the seedbed above 80%, and ventilate and cool down to control the temperature in the shed between 23 and 30°C, and the germination rate is between 60% and 85%. , pick high-quality lateral buds as explants.

[0038] Intercept the stem section with axillary buds from the roots of breadfrui...

Embodiment 2

[0044] With embodiment 1 gained breadfruit side bud as explant, culture method is specifically as follows:

[0045] Take the lateral buds on the mother plant of breadfruit as explant material. The sampled strains were sterilized by pesticides (carbendazim + agricultural streptomycin sulfate 1000 times solution) for 3 days in advance, and then the explants were placed in a triangular flask, added with 4 drops of Tween-203-, and sealed with clean gauze. Rinse with running water for 10-30 minutes until the foam on the surface of the explant is washed away. Then transfer to the aseptic operating table, sterilize with 0.1% mercuric chloride for 4-8 minutes, wash with sterile water for 4-5 times, each time for 1-2 minutes with sterile water, drain the water and wait for inoculation.

[0046] Put the sterilized lateral buds on the cluster bud induction medium, and culture them under 1800lux light at 28±2°C to induce cluster buds. The light cycle is 12h / d, and the medium formula is M...

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Abstract

The invention relates to the technical field of tissue culture and high-throughput breeding of seedlings, in particular to a high-throughput breeding method for breadfruit seedlings. The method comprises the following steps: performing cuttage on root sections of breadfruit mother plants, taking stem sections growing on the root sections to prepare explants, and cleaning, disinfecting and sterilizing the explants; inoculating a callus induction culture medium with the explants for callus induction to obtain stem segments with callus; inoculating a cluster bud induction culture medium with the stem segments with the callus for proliferation and differentiation of cluster buds to obtain cluster buds; and dividing the cluster buds, and inoculating a rooting culture medium with the cluster buds for rooting to obtain complete plants. According to the method, the root sections of the mother plants are used for cuttage, and the stem sections growing on the roots are used as explant materials, so that the cluster buds can be induced to obtain the cluster buds.

Description

technical field [0001] The invention relates to the technical field of tissue culture and high-throughput breeding of seedlings, in particular to a high-throughput breeding method for breadfruit seedlings. Background technique [0002] Breadfruit Artocarpus altilis (Parkinson) Fosberg, also known as bread tree, English name breadfruit, is a perennial tropical rare fruit tree species or woody food crop of the genus Jackfruit in the family Moraceae, and has the reputation of "the tree that grows bread". Native to Polynesia and the West Indies in the South Pacific, it is the main food crop in the area. Breadfruit is rich in nutrition. It is not only the main food crop in tropical areas, but also an important source of total sugar, reducing sugar, protein, ascorbic acid, dietary fiber, and minerals. It can be used not only as a food crop in the south, but also as a characteristic tropical fruit. In addition, the mature fruit is versatile, can be boiled, sliced ​​and roasted or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G2/10A01G17/00
CPCA01H4/001A01H4/00A01G2/10A01G17/005
Inventor 吴刚吉训志苏兰茜秦晓威白亭玉孟倩倩谭乐和
Owner SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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