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Method for culturing and propagating tissues of Taxodium Zhongshanha 302

A technology of tissue culture and culture medium, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of cumbersome cultivation process and complicated material collection methods

Inactive Publication Date: 2012-11-07
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Invention patent CN101347099A discloses a rapid in vitro multiplication method of bald cypress, but the method selects mature seeds and obtains mature embryos through a complicated process, the method of drawing materials is complicated, and the cultivation process is cumbersome, and it is only applicable to the multiplication of bald cypress species, while It cannot be used in Zhongshan fir strains by simply changing
[0005] At present, the research on the tissue culture rapid propagation of Zhongshan fir is still blank at home and abroad. The present invention provides a kind of propagation method of Zhongshan fir 302 tissue culture, which is simple and quick to operate, greatly improves the proliferation rate and proliferation speed, thereby solving Zhongshan fir 302 is in demand in urban landscaping, water conservation, soil and water conservation, green landscape passages, ecological construction, etc.

Method used

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  • Method for culturing and propagating tissues of Taxodium Zhongshanha 302
  • Method for culturing and propagating tissues of Taxodium Zhongshanha 302
  • Method for culturing and propagating tissues of Taxodium Zhongshanha 302

Examples

Experimental program
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Effect test

Embodiment 1

[0041] 1. On May 30, 2010, that is, the 15th day after the branches of Zhongshan Fir 302 turned from green to red completely, 10 healthy and pest-free young shoots (top shoots) of the year were selected, and the side branches were cut off, and the new shoots were cut off. Cut off all leaves in half.

[0042] 2. Rinse the explants with clean water, scrub the surface with a brush dipped in a little washing powder, then rinse with tap water for 3 times, then rinse with "Amway" lotion for 30 minutes, and then rinse with tap water for 30 minutes , 84 disinfectant (diluted 50 times) on a shaker for 10 minutes to sterilize. After rinsing with running water for 20 minutes, sterilize with 75% ethanol for 50 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 12 minutes, and finally wash 4 times with sterile water, shaking for 3 minutes each time. Cut the sterilized explants into small sections of 0.5-1.0cm long, cut out 100 stem sections in total, and ino...

Embodiment 2

[0049] 1. On June 19, 2010, that is, the 35th day after the branches of Zhongshan Fir 302 turned from green to red completely, select 10 healthy and pest-free young shoots (top shoots) of the year, cut off the side branches, and cut off the new shoots. Cut off all leaves in half.

[0050] 2. Rinse the explants with clean water, scrub the surface with a brush dipped in a little washing powder, rinse with tap water for 5 times, then rinse with "Amway" lotion for 30 minutes, and then rinse with tap water for 30 minutes , 84 disinfectant (diluted 50 times) on a shaker for 10 minutes to sterilize. After rinsing with running water for 30 minutes, sterilize with 75% ethanol for 60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 12 minutes, and finally wash 4 times with sterile water, shaking for 5 minutes each time. Cut the sterilized explants into small sections of 0.5-1.0cm long, cut out 100 stem sections in total, and inoculate them in the induct...

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Abstract

The invention discloses a method for culturing and propagating tissues of Taxodium Zhongshanha 302. The method comprises the following steps: (1) selecting an explant and disinfecting the explant; (2) improving an original WPM (wood plant medium) culture medium; (3) after cutting the processed explant, performing primary induction and culture for stems; (4) performing secondary multiplication andculture for new tips obtained in the step (3); (5) performing rooting induction culture for the rootless test-tube plantlet obtained in the step (4); and (6) performing domesticating hardening off culture for the aseptic seedlings obtained in the step (5). The propagating method is adopted to culture seedlings of the Taxodium Zhongshanha 302, the propagating rate is 102-110 times and the seedlingculturing time is short.

Description

technical field [0001] The invention specifically relates to a tissue culture propagation method of Zhongshan fir variety 302 (Taxodium hybrids 'Zhongshansha302'), which belongs to the technical field of woody plant seedling propagation. Background technique [0002] Zhongshan fir (Taxodium hybrids'Zhongshansha'), a general term for the excellent interspecific hybrid clones of Taxodiaceae (Taxodiaceae), composed of T.distichum, T.ascendens, Mexican cypress Cedar (T.mucronatum) three species of cross-breeding. Semi-evergreen tall tree with prominent trunk, conical or umbrella-shaped crown, compact and plump. The leaves are dense, pinnately compound, strip-shaped, 0.6-1.0 cm long, and spirally scattered on the branchlets, not in two rows. Dioecious, same plant. Female cones grow on the top of new branches, singly or in clusters of 2-3, spherical when mature, 3.5-5.0mm long. The male cones grow on the twigs and are elliptical when mature, 3.0-5.0mm long. The flowering peri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 陆小清李乃伟陈永辉李云龙王传永
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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