45 results about "Taxodium" patented technology

The invention discloses an in-vitro quick propagating method of Taxodium.distichum, which pertains to the field of plant tissue culture. The method of the invention comprises the following steps: (1) taking mature embryos of sterilized Taxodium.distichum seeds as explant; (2) induction culture of adventitious buds; (3) elongation culture of adventitious buds; (4) propagation culture of adventitious buds; (5) culture of sound plantlets; (6) induction culture of adventitious roots; (7) formation of regenerative plants; (8) plantlets hardening and transplanting; wherein the basic culture medium applied to each culture stage is SH culture medium; the growth regulator added at each culture stage has the most suitable use amount and proportion which are selected by orthogonal design experiments. The test-tube plantlets of the Taxodium.distichum bred by the method of the invention have strong growth capacity, high propagation coefficient, up to 80% of the inducing rooting rate, approximate 90% of the transplant survival rate, and can be used for large-scale and industrialized production.

The invention discloses a Taxodium Zhongshanha 406 mesophyll protoplast separation, purification and high-efficiency conversion method. The method comprises the following steps: 1, carrying out dark pretreatment on a material tender leaves of 30 days-old good-growing tissue culture seedlings of Taxodium Zhongshanha; 2, separating and purifying mesophyll protoplasts; 3, extracting 50-80 [mu]g of a plasmid, which is a vector p2FGW7.0, from 15-20 mL of an overnight cultured bacterium liquid; and 4, converting the protoplasts through PEG mediation. The Taxodium Zhongshanha 406 mesophyll protoplast separation, purification and high-efficiency conversion method has the advantages of simplicity and easiness in operation, obtaining of massive, complete and pure Taxodium Zhongshanha mesophyll protoplasts, and high plasmid conversion rate reaching 80%. The method solves a problem that the gene functions of the Taxodium Zhongshanha cannot be deeply researched, provides a certain reference for the establishment of other coniferous species protoplast instantaneous expression systems, and has great application values in the field of forest tree gene engineering.

A pezicula sp. SC1337 strain, and a method of preparing trisaccharide ester derivatives by utilizing the strain are disclosed. The accession number of the pezicula sp. SC1337 is GDMCC No:60144. The pezicula sp. SC1337 is separated from endophytes of branches of taxodium distichum (L.) Rich., and can generate five compounds having obvious inhibiting activity on twelve common plant pathogenic fungi and having fresh-keeping effects on fruits, and therefore the strain can be used for preparing the five compounds having obvious inhibiting activity on the twelve common plant pathogenic fungi and having fresh-keeping effects on fruits, the compounds are used for control of the plant pathogenic fungi and fresh-keeping of fruits, and the strain has a wide application prospect.

The invention relates to a method for biologically treating sewage through cation exchange, adsorption and filtering by using rice hull carbon. The method is characterized by comprising the following steps of: digging earthwork with the volume of 1.5m*1.5m*1.5m in a ditch and pond water front zone; laying the rice hull carbon and the original soil on a backfill soil layer at high intervals of 5cm; and planting taxodium distichum in the earthwork after the backfill soil layer is laid. Water flow flows through an artificial carbon-soil interlayer by using the water height of a natural water body, so that the water body is fully contacted with a carbon material; cation in the water body is exchanged with a functional group on the surface of the carbon material and is fixed on the surface of the carbon material; and the cation which is exchanged and trapped on the carbon material is absorbed by a root system of the taxodium distichum and then is discharged out of a water body system. Compared with the conventional artificial wetland treating technology, the method has the advantages that: nitrogen-phosphorous removal efficiency is obviously improved; simultaneously, the cation is frequently exchanged in the soil improved by the rice hull carbon; microbial communities have diversity; fertilizer fixing capacity and plant growing capacity are enhanced; and plants such as the taxodiumdistichum and the like are better promoted to grow.

The invention discloses a tissue culture propagation method of taxodium hybrids'zhongshansha118'. The method comprises the following steps: (1) selecting and disinfecting an explant; (2) improving a 1 / 2 MS culture medium; (3) cutting the treated explant, carrying out stem primary induction culture and treating in a high-voltage electrostatic field (HVEF); (4) carrying out subculture multiplication culture on adventitious buds obtained in the step (3); (5) carrying out rooting induction culture on rootless test tube seedlings obtained in the step (4); and (6) carrying out domestication on sterile seedlings obtained in the step (5) and transplanting. When the tissue culture propagation method disclosed by the invention is used for cultivating seedlings of taxodium hybrids'zhongshansha118', the seedling growing time is short, the multiplication rate is 89-92 times, the rooting rate is 90%, the average rooting quantity is 4.0-4.1, and the domestication survival rate is up to 95%. With the method, seedlings of taxodium hybrids'zhongshansha118' can be effectively propagated and the market requirement on seedlings of taxodium hybrids'zhongshansha118' can be met to the great extent.

The invention provides a taxodium zhongshansha 406 hard wood cutting simple culture method, and belongs to the technical field of vegetative propagation. The taxodium zhongshansha 406 hard wood cutting simple culture method is easy to implement, economical and practical. The method mainly includes the steps that a cutting bed is built by means of a common farmland, wherein a cutting matrix is a mixture of river sand and farmland fine soil, and the volume ratio of the river sand to the farmland fine soil is 1 to 1; by means of cutting orchard winter pruning, cuttings which are 10 cm long are obtained through cutting, and two cuts are carved in the surface layer, 0-4cm long, on the base portion of each cutting through a single-side blade in the vertical direction of the cutting; the cuttings are processed through rooting hormone before cutting; the cuttings are covered with plastic membranes after cutting and kept moist; during transplanting, the roots of the cuttings have few cutting matrixes. By means of the method, good characteristics of quick growth, saline-alkaline tolerance, waterlogging resistance and the like of taxodium zhongshansha 406 can be kept, the rooting rate of cutting is high, and the method is easy to implement. The utilization rate of branches of unit area in a taxodium zhongshansha 406 cutting orchard is fully increased, the number of cultivated seedlings is increased, and the seedling culturing cost is lowered.

The invention discloses a ThHAM1 gene related to development of roots of taxodium zhongshansha and application for the ThHAM1 gene and belongs to the technical field of plant gene engineering. A nucleotide sequence of the ThHAM1 gene is shown as SEQ ID NO. 1, and an amino acid sequence of an expression protein of the ThHAM1 gene is shown as SEQ ID NO. 3. Populus davidiana x P.bolleana is convertedby constructing a gene GbF3'H1 vector, compared with a control plant, the ThHAM1 gene performs efficient expression in the Populus davidiana x P.bolleana, following the situation that variation of aphenotype occurs, a growth speed of a plant is slowed with roots being most obvious, the number of adventitious roots is reduced, a length is shortened, and the expression quantity of a target gene isincreased by 200-500 times, so that illustrated that the ThHAM1 gene is an important gene for regulating and controlling development of the plant roots and influencing a plant morphology, and the ThHAM1 gene has an important application value in the field of forest tree gene engineering and can be used for regulating a plant growth and development speed or the plant morphology.

The invention discloses a method for culturing and propagating tissues of Taxodium Zhongshanha 302. The method comprises the following steps: (1) selecting an explant and disinfecting the explant; (2) improving an original WPM (wood plant medium) culture medium; (3) after cutting the processed explant, performing primary induction and culture for stems; (4) performing secondary multiplication andculture for new tips obtained in the step (3); (5) performing rooting induction culture for the rootless test-tube plantlet obtained in the step (4); and (6) performing domesticating hardening off culture for the aseptic seedlings obtained in the step (5). The propagating method is adopted to culture seedlings of the Taxodium Zhongshanha 302, the propagating rate is 102-110 times and the seedlingculturing time is short.

The invention discloses a seedling raising by cutting method of taxodium 'Zhongshansha' in green house containers. The seedling raising by cutting method comprises the steps of preparing seedbeds, performing cutting and performing management after cutting. The invention also discloses substrates for seedling raising by cutting of taxodium 'Zhongshansha' in green house containers. The substrates consist of the following components of 76-84% of turf, 7-9% of perlite, 5-7% of carbonized rice chaff, 3-5% of vermiculite and 1-3% of controlled-release fertilizers. A high bed cultivation mode is combined with adjustment of components of the substrates, control of temperature and humidity during the seedling raising period is guaranteed, and the substrates consisting of the turf, the perlite, thecarbonized rice chaff, the vermiculite and the controlled-release fertilizers are combined for use, so that the survival rate can be greatly increased, cutting seedlings of the same year can be sold in the same year to generate economic benefits and can be completely sold before plum rain season the next year, production of next batch of cutting seedlings of the taxodium 'Zhongshansha' in glass green houses is not influenced, transformation and upgrading of a conventional seedling raising manner of forestry seedlings to high-quality development are realized, and a high-efficient development mode of being short, stable and fast, of the forest seedling industry can be realized.

The present invention discloses a taxodium hybrid 'zhongshanshan' ThSHR3 gene for promoting plant growth and an application of the taxodium hybrid 'zhongshanshan' ThSHR3 gene, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the ThSHR3 gene is shown in SEQ ID NO.1 and an amino acid sequence of an expressed protein is shown in SEQ ID NO.2. Sequence alignment and evolution analysis of the ThSHR3 gene show that the ThSHR3 gene belongs to a SHR gene subfamily. Populus davidiana x P.bolleana is transformed by constructing a gene pH35GS-ThSHR3 vector. Compared with the control plant, the ThSHR3 gene is highly expressed in the Populus davidiana x P.bolleana, the expression amount of the target gene is increased by 500 times or more, and the plant growthrate is significantly higher than that of the control plants, indicating that the ThSHR3 gene is an important gene that regulates plant development and affects plant morphology, has important application value in the field of forest genetic engineering and can be used to adjust plant growth and development speed or plant morphology.

The invention relates to the technical field of ecological management, and provides a reservoir tail hydro-fluctuation belt wetland restoration method based on forest habitat. The method comprises the following steps of S1, selecting saplings: selecting a flooding-resistant large arbor with the height that the tree top can exceed the highest water level by 20-80cm after the tree top is submerged in the highest water level after planting, the large arbor comprises one or more of pond fir, Chinese tipping, taxodium zhongshanha and taxodium falcatum; and S2, planting, wherein planning small-sized trees resistant to water logging along a riparian zone of a reservoir tail hydro-fluctuation belt, and planning the large arbors below small arbors at a certain distance. According to the method, the spatial heterogeneity of the reservoir tail hydro-fluctuation belt is improved, and the original vegetation condition can be quickly and completely recovered after the reservoir tail hydro-fluctuation belt goes through a long-time highest water level environment.

The invention discloses a cuttage technology optimization-based high-efficient cultivation method based on taxodium Zhongshanha container seedlings, and belongs to the technical field of taxodium Zhongshanha cuttage seedling raising. The method comprises the following steps: performing cuttage on taxodium zhongshanha cutting slips by adopting degradable non-woven fabric cuttage seedling raising bags in early June and middle June, and after cuttage is completed, putting cuttage seedlings into a shading shed for spraying management; and after 20-30 days, planting the cuttage seedlings and the cuttage seedling raising bags into a large-specification seedling raising container, and after 2-3 months of seedling refining management and later maintenance, obtaining high-quality taxodium zhongshanha container seedlings with the height being 50 cm or above in the current year. According to the method, seedling bag cuttage replaces a cuttage bed type cuttage method, so that the ventilation condition of a cutting rooting environment is improved, and rooting of cutting slips is promoted; and a one-bag and one-seedling mode is adopted, so that after cuttage seedlings root, containers can be replaced for planting in time, and damage to root systems of the cuttage seedlings cannot be caused.

The invention discloses an in-vitro propagation method of taxodium hybrids 'zhongshansha136'. The in-vitro propagation method includes the following steps that (1) explants are selected and disinfected; (2) a 1 / 2MS culture medium is improved; (3) the processed explants are cut, and stem primary induction culture and high-voltage electrostatic field (HVEF) processing are conducted; (4) secondary multiplication culture is conducted on adventitious buds obtained in the step (3); (5) root induction culture is conducted on rootless test-tube plantlets obtained in the step (4); (6) domestication seedling and transplanting are conducted on aseptic seedlings obtained in the step (5). The tissue culture propagation method is used for conducting seedling on the taxodium hybrids 'zhongshansha136', the seedling time is short, the reproduction rate is increased by 88-93 times, the rooting rate is 89%, the average root number is 4.1, the seedling survival rate reaches 95%, taxodium hybrids 'zhongshansha136' seedlings can be obtained efficiently, and the requirement for the taxodium hybrids 'zhongshansha136' seedlings of the market is met to a large extent.

The invention relates to the field of plant planting, in particular to a method for interplanting of iris hybrids 'Louisiana' in a taxodium 'Zhongshanshan' forest. The method comprises the following steps that a, low-lying wetland with water depth lower than or equal to 40 centimeters is selected, ploughing is conducted, planting oil under water is flattened, and hole digging and land preparationare conducted; b, taxodium 'Zhongshanshan' soil ball seedlings or container seedlings with the diameter at breast height of 3 centimeters are selected and transplanted to the low-lying wetland, and the plant spacing in the rows is higher than or equal to 4 meters*6 meters; c, in the current year of taxodium 'Zhongshanshan' transplantation, through a plant division method, the iris hybrids 'Louisiana' is planted in the low-lying wetland under the taxodium 'Zhongshanshan' forest according to the plant spacing in the rows of 40 centimeters*40 centimeters. For the current condition of low utilization rate of the low-lying wetland in current production, the method for interplanting of the iris hybrids 'Louisiana' in the taxodium 'Zhongshanshan' forest is provided, the ornamental value under theforest is increased, and the utilization efficiency of land per unit area is improved.

The invention provides a hydro-fluctuation belt biological treatment method. The method comprises steps of planting Taxodium hybrid 'Zhongshanshan' in areas having 10 meters or lower elevation difference with the highest elevation of the hydro-fluctuation belt when the hydro-fluctuation belt is exposed out of the water surface, hybrid-planting fodder mulberry, Bermuda grass or entada pstems between the Taxodium hybrid 'Zhongshanshan'. By the use of the biological treatment method, high plant survival rate can be achieved; and great and stable biological treatment effect can be realized.

The invention discloses a method for performing cutting and seedling raising on taxodium hybrid. The method comprises the following steps: building a seedling raising pond; preparing a floating seedling raising tray; preparing a cutting matrix; preparing a rooting medicament; preparing cutting shoots; soaking the cutting shoots; cutting; laying out the seedling tray; injecting water; transferringseedlings. According to the method disclosed by the invention, the excellent properties of quick growth, saline-alkali resistance, water dampness resistance and the like of the taxodium hybrid can bemaintained, a cutting rooting ratio is high, and the method is simple and feasible. By adopting the method, an utilization ratio of branches of a taxodium hybrid cutting orchard in the unit area is fully increased, the quantity of cultivation of seedlings is increased, the seedling raising cost is reduced, labor saving and cost saving of seedling raising can be realized, non-renewable resources are conserved, an ecological environment is protected, and low-carbon cycle is realized; in addition, cutting and seedling raising can be carried out by adopting the seedling tray, so that the rooting ratio and survival ratio of the cutting shoots can be obviously increased, the seedlings are in order and strong, and the roots are developed.

The invention relates to the technical field of asexual reproduction of plants and particularly discloses a method for raising seedlings of taxodium distichum*T.Mucronatum'zhongshanshan' by cutting ata low cost and at a high outplanting rate. The seedling culturing method comprises steps as follows: S1, preparation of nursery land; S2, preparation of cuttings; S3, cottage; S4, management of the rooting phase of cutting seedlings; S5, management after rooting of the cutting seedlings; in preparation of nursery land in S1, the cutting medium of the nursery land is yellow soil drawn on local resources, that is, a layer of about 5 cm thick yellow soil is paved on a seed bed which has been sterilized, disinfected and disinfested. The method has the remarkable benefits as follows: 1) the cutting medium is obtained conveniently: local fresh yellow soil is adopted as the cutting medium; 2) facilities and equipment are simple, all that is required is to establish simple shading and irrigationfacilities; 3) the cost is saved, the input and maintenance cost of full-automatic irrigation equipment is reduced, the seedling culturing cost is reduced by 50% or above; 4) the outplanting rate is increased, cut rooting seedlings can be remained in a nursery and continuously cultivated for one year, damage of the seedling transplanted out of the nursery is reduced, and the outplanting rate is increased by 10% or above.

The invention provides Taxodium hybrid 'zhongshanshan' essential oil and an application thereof to inhibition of plant pathogens. The Taxodium hybrid 'zhongshanshan' essential oil is prepared from leaves of Taxodium hybrid 'zhongshanshan' by extraction with a steam distillation method, and the essential oil has a good inhibition effect on the plant pathogens such as curvuloria lunata of rice, alternaria alternata, valsa mali, physalospora piricola, rhizoctonia solani, rhizoctonia solani kuha, colletotrichum gloeosporioides of ophiopogon japonicus, sclerotinia sclerotiorum, gaeumannomyces graminis and fusarium oxysporum vasinfectum.

The invention belongs to the technical field of agriculture, and discloses a taxodium Zhongshanha cutting medium and a seedling raising method thereof. The taxodium Zhongshanha cutting medium is prepared by mixing and fermenting taxodium waste, pine waste and loess according to the mass ratio of (2-5): (3-6): 2, and then adding with perlite. The method specifically comprises the following steps: S1, taxodium waste, pine waste, loess and perlite are collected; S2, the taxodium waste, the pine waste and loess are mixed according to the mass ratio of (2-5): (3-6): 2 and subjected to stack retting, and the mixture is rotted; S3, unrotten sundries in the S2 are picked out, and humus soil of taxodium and pine is obtained; S4, the humus soil and perlite are evenly mixed according to the volume ratio of 8: 2, and the taxodium Zhongshanha cutting medium is obtained; and S5, the medium in the step S4 is placed into a container bag for later use. According to the technical scheme, different preparation methods are adopted for the taxodium Zhongshanha cutting medium, so that the seedling culture process is simplified, and the production cost is reduced.

The invention discloses an in-vitro quick propagating method of Taxodium.distichum, which pertains to the field of plant tissue culture. The method of the invention comprises the following steps: (1) taking mature embryos of sterilized Taxodium.distichum seeds as explant; (2) induction culture of adventitious buds; (3) elongation culture of adventitious buds; (4) propagation culture of adventitious buds; (5) culture of sound plantlets; (6) induction culture of adventitious roots; (7) formation of regenerative plants; (8) plantlets hardening and transplanting; wherein the basic culture medium applied to each culture stage is SH culture medium; the growth regulator added at each culture stage has the most suitable use amount and proportion which are selected by orthogonal design experiments. The test-tube plantlets of the Taxodium.distichum bred by the method of the invention have strong growth capacity, high propagation coefficient, up to 80% of the inducing rooting rate, approximate 90% of the transplant survival rate, and can be used for large-scale and industrialized production.

The invention provides a root-divided alternative irrigation method adopting a zeolite salt isolation layer. The method includes the step of laying the salt isolation layer. A tree leaf layer, a rubble layer, a fine stone layer and a nutrient layer are sequentially laid on the salt isolation layer from bottom to top. The invention further provides application of the root-divided alternative irrigation method adopting the zeolite salt isolation layer to planting Taxodium Zhongshanha in saline-alkali soil. By means of the root-divided alternative irrigation method, the irrigation water amount per mu is reduced by 40-50% compared with the common irrigation water amount, and salification and salt return of a planting area soil root layer are greatly limited, so the salt content in 0-60 cm of planting area soil is 0.08-0.12%. Unnecessary transpiration of plants is greatly reduced. Taking Taxodium Zhongshanha as an example, the transpiration speed of Taxodium Zhongshanha is reduced by 17-26%.

The invention relates to a cultivation method for increasing the summer transplanting survival rate of engineering seedlings of north American taxodium distichum. The cultivation method comprises the following specific implementation steps of: 1, thinning all branches of the engineering seedlings sprouted with branches, except top tip branches, from bottom to top; 2, digging in a forest land to be planted, transplanting the engineering seedlings treated in the step 1 in holes, covering soil on the surface of soil balls of the engineering seedlings processed in the step 1, and fixing the engineering seedlings; and 3, immersing roots of the engineering seedlings treated in the step 2 by using water for one month. According to the invention, the cultivation method is simple and easy for implementation; the transplanting survival rate of the north American taxodium distichum can be increased to be above 90%; furthermore, the method is particularly applied to poor transplanting conditions for accelerating water transpiration of plants in the high-temperature environment in summer; and cultivation quality requirements of the greening and afforestation projects in summer can be satisfied.

The invention discloses an extraction methodof taxodium zhongshanense essential oil. The method comprises the following steps: freezing, ball-milling and crushing taxodium Zhongshanense leaves, and carrying out supercritical carbon dioxide extraction separation to obtain the taxodium Zhongshanense essential oil and byproducts of taxodium Zhongshanense heavy oil and taxodium Zhongshanense wax; thesupercritical carbon dioxide extraction is carried out at a low temperature; and according to the method, the natural components of the taxodium zhongshanense essential oil can be effectively protected from being damaged through steam distillation, no chemical solvent is added, and the obtained taxodium zhongshanense essential oil is natural, free of pollution and pure and rich in smell, so that atechnical and product quality guarantee is provided for production of the taxodium zhongshanense essential oil and development of related products. The method has the characteristics that the naturalcomponents of the taxodium zhongshanense essential oil are effectively protected from being damaged, and no chemical solvent is added.

The invention relates to the technical field of pesticide synergists, and discloses a botanical synergist of emamectin benzoate. The synergist is prepared by mixing taxodium zhongshanha essential oil with emamectin benzoate according to a mass ratio of (10-50): 1 for use, and can be used as an insecticide for preventing and controlling spodoptera frugiperda. The synergist can remarkably improve the pesticide effect of the emamectin benzoate, can reduce the dosage of the emamectin benzoate under the same prevention effect, overcomes and delays the pesticide resistance of pests, and has the significance of safety, environmental protection, reduction of environmental pollution, remarkable economic benefits and environmental protection.

The invention discloses a taxodium Zhongshanha container seedling and field integrated cultivation method, and belongs to the technical field of taxodium Zhongshanha cultivation. The method comprises the following steps: selecting 1-year-old taxodium zhongshanha cutting seedlings, after classification, planting the seedlings in a cutting bed temporarily in time, planting container seedlings while taking the container seedlings during field planting, and conducting watering in time after planting; performing deep ploughing on a clayed soil area before winter, performing leveling and bedding in early spring of the next year, and directly performing field improvement and bedding on a sandy soil area before operation in early spring; carrying out field ditching and shallow burying after the container seedlings are planted; performing spray irrigation on shallowly-buried container seedlings, manually pulling out weeds in the container in time after the seedlings survive, and pruning lateral branches and competitive branches in the growing period; and finally outplanting the seedlings in the form of container seedlings. Through two technical links of container planting and field shallow burying management and protection, the advantages of two cultivation methods of container seedling cultivation and field cultivation are combined, and the method has the advantages that cultivation conditions are controllable, the survival rate of seedlings after field planting is high, the growth vigor is consistent, and seedling outplanting is not limited by seasons.

The invention discloses a method for separating, purifying and instantaneously and efficiently converting root, stem and leaf protoplasts of taxodium distichum. The method comprises the following steps: 1) a taxodium distichum seedling with 1-3 rounds of grown leaves is selected and cultured for 24 hours in a dark place, cleaning is conducted once with 75% alcohol firstly, then cleaning is conducted with deionized water for three times, and then the leaves, stem segments and roots of the taxodium distichum seedling are separated by a blade; (2) the roots, stems and the leaves of the taxodium distichum are subjected to enzymolysis through cellulase and pectinase to obtain the protoplasts, and the obtained protoplasts are separated, purified and pretreated; and (3) the protoplasts of different tissue are taken as receptors, a transient expression vector containing an exogenous gene is transferred into the protoplasts through a PEG-mediated transformation method, and through culturing, the exogenous gene is expressed in the protoplasts. The method is easy to operate and easy to implement, a large number of the complete and pure root, stem and leaf protoplasts of the taxodium distichumcan be obtained, the conversion efficiency reaches up to 50% or above, and the technical basis is provided for transient expression detection of a taxodium distichum gene, protoplast fusion and the like.

The invention, which belongs to the technical field of biology, relates to a detection primer, a kit and a detection method for endophytic fungi diversity of taxodium zhongshanshan. The detection primer comprises a first primer pair and a second primer pair. The sequences of the first primer pair are shown as SEQ ID NO.1 and SEQ ID NO.2; and the sequences of the second primer pair are shown as SEQID NO.3 and SEQ ID NO.4. The detection primer provided by the invention is obtained by analyzing and designing the diversity of leaf fungi of the taxodium zhongshanshan and has high sensitivity and detection concentration. The detection method provided by the invention is a detection scheme with simple steps and low cost; and a theoretical reference can be provided for detecting the diversity ofendophytic fungi in different varieties of leaves in different regions of the taxodium zhongshanshan.