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Taxodium hybrid 'zhongshanshan' essential oil and application thereof to inhibition of plant pathogens

A technology of plant pathogenic bacteria and Zhongshan fir, which is applied in the fields of application, plant growth regulator, botanical equipment and methods, etc., can solve the problems of rare in-depth development and application research of Zhongshan fir, and the absence of extraction of effective substances from Zhongshan fir plants, etc. , to achieve the effect of effective utilization, good prevention and control, and pollution reduction

Active Publication Date: 2019-04-26
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the utilization of Zhongshan fir is mainly based on the plant characteristics of the tree itself. There are few in-depth development and application research on Zhongshan fir, and there is no research on the extraction of effective substances in Zhongshan fir plants.

Method used

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  • Taxodium hybrid 'zhongshanshan' essential oil and application thereof to inhibition of plant pathogens
  • Taxodium hybrid 'zhongshanshan' essential oil and application thereof to inhibition of plant pathogens
  • Taxodium hybrid 'zhongshanshan' essential oil and application thereof to inhibition of plant pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The leaves of Zhongshan fir plants were collected in the Agricultural Ecological Industrial Park of Fengtai County, Anhui Province.

[0037] 3kg of fresh Zhongshan fir leaves were pulverized and placed in a volatile oil extraction device, and distilled water was added until the ratio of solid to liquid was 1:10, and mixed;

[0038] Then, add 60ml of n-hexane to the extraction device, and distill and extract at 60°C for 6h;

[0039] After cooling, separate the layers, collect the n-hexane layer, and concentrate under reduced pressure to remove the n-hexane to obtain the essential oil of Chinese fir, which is transferred to a brown sample bottle for storage at 4°C.

Embodiment 2

[0041] The leaves of Zhongshan fir plants were collected in the Agricultural Ecological Industrial Park of Fengtai County, Anhui Province.

[0042] 3kg of fresh Zhongshan fir leaves are pulverized and placed in a volatile oil extraction device, distilled water is added until the ratio of solid to liquid is 1:15, and mixed;

[0043] Then, add 100ml of n-hexane to the extraction device, and distill and extract at 60°C for 6h;

[0044] After cooling, separate the layers, collect the n-hexane layer, and concentrate under reduced pressure to remove the n-hexane to obtain the essential oil of Chinese fir, which is transferred to a brown sample bottle for storage at 4°C.

Embodiment 3

[0046] The leaves of Zhongshan fir plants were collected in the Agricultural Ecological Industrial Park of Fengtai County, Anhui Province.

[0047] 3kg of fresh Zhongshan fir leaves were pulverized and placed in a volatile oil extraction device, and distilled water was added until the ratio of solid to liquid was 1:10, and mixed;

[0048] Then, add 60ml of petroleum ether to the extraction device, and distill and extract at 60°C for 6h;

[0049] After cooling, the liquid was separated, the organic layer was collected, and the petroleum ether was removed by concentration under reduced pressure to obtain the essential oil of Chinese fir, which was transferred to a brown sample bottle and stored at 4°C.

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Abstract

The invention provides Taxodium hybrid 'zhongshanshan' essential oil and an application thereof to inhibition of plant pathogens. The Taxodium hybrid 'zhongshanshan' essential oil is prepared from leaves of Taxodium hybrid 'zhongshanshan' by extraction with a steam distillation method, and the essential oil has a good inhibition effect on the plant pathogens such as curvuloria lunata of rice, alternaria alternata, valsa mali, physalospora piricola, rhizoctonia solani, rhizoctonia solani kuha, colletotrichum gloeosporioides of ophiopogon japonicus, sclerotinia sclerotiorum, gaeumannomyces graminis and fusarium oxysporum vasinfectum.

Description

technical field [0001] The invention relates to the field of fungicides, in particular to Zhongshan fir essential oil and its application in inhibiting plant pathogenic bacteria. Background technique [0002] Taxodium hybrid 'zhongshanshan' belongs to Taxodiaceae (Taxodiaceae) and is composed of T distichum (Linn) Rich, Tascendens Brong and Mexican T mucronatum Tenore. ) The general term for the excellent clones obtained through cross breeding among the three tree species. [0003] Zhongshan fir is a semi-evergreen tall tree with a conical to broad oval crown, dense leaves, straight trunk, fast-growing, salt-alkali-resistant, strong resistance to separation, less pests and diseases, excellent material, long green period, and good landscape effect, etc. advantage. Zhongshan fir not only has good ornamental value, but also is conducive to water conservation and soil and water conservation. It is a new type of greening tree species. At the same time, the fallen leaves of Zho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/06A01P3/00
CPCA01N65/00A01N65/06
Inventor 操海群赵振宇高全钱益波廖敏肖金京
Owner ANHUI AGRICULTURAL UNIVERSITY
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