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Method for injecting low-energy N<+> for mutation breeding hericium erinaceus strain and bred strain

A technology of Hericium erinaceus and strains, which is applied in the fields of bioengineering and edible and medicinal mushrooms, can solve problems such as unseen Hericium erinaceus strains, etc., and achieve the effects of improving efficiency and probability of positive mutation, good mutagenesis effect and not easy to degrade.

Active Publication Date: 2014-05-21
JIANGSU ANHUI BIO TECH +1
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Problems solved by technology

[0004] Haven't seen any reports on the mutagenesis of Hericium erinaceus strains by using low-energy nitrogen ion implantation technology

Method used

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  • Method for injecting low-energy N&lt;+&gt; for mutation breeding hericium erinaceus strain and bred strain

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Embodiment Construction

[0022] A method for mutagenesis and breeding of Hericium erinaceus strains by low-energy nitrogen ion implantation, and its steps are:

[0023] l. Screening of starting strains

[0024] Introduce different species of Hericium erinaceus from the main producing areas of Hericium erinaceus (including Hericium erinaceus CAHHFT-703, CAHHFT-705, CAHHFT-706, CAHHFT-707, CAHHFT-7010, CAHHFT-708, Jiangsu Province Microorganism Research Co., Ltd. keeps the collection species He12, and the Hericium erinaceus introduced by Shandong Jinxiang Fungi Research Institute). Inoculate on the Hericium erinaceus solid slant medium (see below for the medium formula), and cultivate for 9 days at 26°C in the dark for activation. Inoculate the activated strains in the center of the solid medium plane, record the time of germination of Hericium erinaceus hyphae, measure the growth rate of hyphae by the cross method, and observe the hyphae morphology (hyphae thickness and density). Then carry out the Heric...

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Abstract

The invention discloses a method for injecting low-energy N<+> for mutation breeding a hericium erinaceus strain and the bred strain. The preservation number of the strain is CGMCC No.8610. The breeding method comprises the following steps: screening a hericium erinaceus starting strain subjected to mutagenesis; selecting a protoplast of the hericium erinaceus starting strain as a material for mutagenesis by injecting low-energy nitrogen ions; injecting nitrogen ions for mutagenesis; screening a mutant strain; verifying genetic trait stability, and the like. A novel hericium erinaceus strain of which the fruiting body yield is higher than that of the parent strain is mutated by the invention, the yield of the hericium erinaceus is improved, and good economic benefits can be created.

Description

Technical field [0001] The present invention relates to the technical field of bioengineering and edible and medicinal fungi, in particular to a method of using low-energy nitrogen ion implantation for mutagenesis to select high-yielding Hericium erinaceus strains, and a mutagenized Hericium erinaceus strain with high fruiting body yield obtained by the above method Mushroom strains. Background technique [0002] Hericium erinaceus belongs to the kingdom of Fungi, Basidiomycota, Agaricus, Agaricus, Russula, Hericium, Hericium erinaceus, Hericium erinaceus, scientific name: Hericium erinaceus , Its fruit body is one of the main raw materials for deep processing of products. Numerous studies have now confirmed that Hericium erinaceus has a variety of physiological functions such as improving immunity, anti-tumor, anti-aging, and reducing blood lipids, especially its unique anti-ulcer effect. It plays an important role in the regeneration and repair of the gastric mucosal epitheli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N15/01C12N1/14C12R1/645
Inventor 汪洁郑惠华陈惠刘广健全卫丰蒋益薛璟田贞乐季红更张晨菊鲁金泉
Owner JIANGSU ANHUI BIO TECH
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