The invention discloses a handling method for inducing citrus
bud mutation through NaN3. The method includes the following steps that NaN3 conditioning fluid is prepared; citrus one-to-two-year-old grafted seedlings are selected, the part, which is not lignified completely, of a
branch to be handled is
cut off, and all leaves and germinal buds of the reserved part of the
branch are removed; all leaf axils of the reserved part of the
branch are incised by a sharp blade; the branch to be handled is wrapped with absorbent paper by one to two
layers, and the wrapped absorbent paper is wetted by the NaN3 conditioning fluid to be saturated through a liquid-moving
machine and the like; the branch is covered with a
preservative film until handling is completed, so that the conditioning fluid is prevented from being evaporated; the wrapped absorbent paper is uncovered, a
plant is placed under normal culture conditions such as the outside of a room to grow, it can be observed that new shoots can germinate and grow from certain leaf axils after five days, and afterwards, variation character observation and statistics of leaf morphology and the like can be conducted. In the
culture growth process after handling, the buds growing at the unhandled part of the
plant need to be removed in time, in accordance with experimental result statistics, the
mutagenesis effect is obvious, and application value can be achieved.