67 results about "Culture growth" patented technology

A method of making sampling tubes containing culture growth media by loading the sample tubes containing culture media into a tray that holds the sample tubes, placing the sample tube trays into a rack with shelving to hold the trays and tubes at a predetermined angle, and sterilizing and cooling the sample tubes in an autoclave or inspissator. The culture growth media solidifies at the predetermined slant angle and the sample tube trays are loaded into the packaging box used for shipment. The trays are subsequently used by the end user for processing microbial growth, including storing and collecting data about microbial samples.

The invention relates to a tissue culture and fast-propagation technology for virus-free plantlets of red bud taros. Healthy and strong corms without damage by diseases and insects are selected, sterilized and disinfected; corm tips of 0.2mm are picked out and then inoculated into the culture medium for differentiated culture; cluster buds are cultured, the top ends of the cluster buds are cut down and then the cluster buds are placed in a proliferation culture medium for fast-propagation; and when the leaves of the cluster buds spread, the cluster buds are placed in a rooting culture medium for strong plantlet rooting culture and later transferred to plantlet hardening. As for the method, a virus eradication technology is adopted twice in propagation to pick out the corm tips of the test tube plantlets and the virus eradication effect is improved. In the preparation of the culture medium, the formula is optimized, thus being beneficial to the growth of the test tube plantlets. The defects of large usage quantity of seeds and low cost when the corms are used as propagating materials in tradition are overcome; the culture growth conditions can be controlled artificially, therefore the method is free from the influence of natural conditions; and the usage quantity of materials is less, the culture materials are economical, the growth cycle is short, the propagation coefficient is high, the production cost is lowered, the variety property is maintained, the yield and the quality are improved, and industrial plantlet culture and industrialized production can be realized.

An air quality test unit for attachment to a vent of a HVAC system with air flow there through. The air quality test unit is a closeable collection container is used with only one substrate panel. The substrate panel with the collection container has one or more ball and socket pivot hinges coupling the substrate panel to the clip. The air quality test unit is attachable to the vent by the clip. The collection container of the substrate panels capture airborne substances and contaminants emanating from the air flow of the HVAC system vent and is then closed with a detachable cover. A culture growth medium may be disposed in the collection container. The air quality test unit kit provides a user with an air quality test unit and process for shipping the unit to the laboratory for analysis.

The present invention discloses a plant growth culture equipment capable of controlling CO2 concentration. It includes a sealed plant growth chamber component with opening device. It is made of transparent material, one end of its outet tube is communicated with the internal cavity of said plant growth chamber component, and its another end is connected with CO2 gas balance component by successively means of three-way switching valve I, tube I, air pump and air outlet tube, the CO2 gas balance component is connected with internal cavity of said plant growth chamber component by successively means of tube II, air buffer tank, tube IV, CO2 detection component, tube III, three-way switching valve II and inlet tube; two ends of connecting tube are respectively connected with three-way switching valve I and three-way switching valve II. The application of said invention can raise accurate control of CO2 concentration.

The invention relates to a preparation method of a biomass charcoal based acid soil regulator, and belongs to the technical field of a soil regulator. The preparation method disclosed by the invention comprises the steps of: firstly, carrying out fermentation by utilizing livestock manure, wood flour, soil rhizobium and the like to obtain a soil nutrient medium; then carrying out ball milling on dolomite, coal ash and the like to prepare composite stone powder; then mixing the composite stone powder with the soil nutrient medium, and transplanting pea plants to carry out culture growth; activating nutritional ingredients in soil by utilizing the growing process of the pea plants; then carrying out pyrolysis on the pea plants to prepare biomass charcoal; after activating the biomass charcoal, mixing the biomass charcoal with culture soil powder, and transferring a mixture into a granulator to prepare particles; after carrying out packaging and bagging, obtaining the biomass charcoal based acid soil regulator. The soil regulator prepared by the preparation method disclosed by the invention can break soil hardening, loosen the soil and improve gas permeability of the soil, has functions of improving the soil, storing water for drought resistance, reinforcing disease resistance of crops, improving yield of the crops, improving quality of agricultural products and recovering the original ecology of the crops, and greatly improves a planting survival rate and yield of the agricultural products.

The invention discloses a preparation method for a natural bone repairing material capable of accelerating osteanagenesis, and belongs to the technical field of medical materials. The preparation method comprises the following steps: calcining by taking ox bones as raw materials, generating a high-intensity alpha-tricalcium phosphate crystal form and a high-degradability high-bioactivity excellent-osteoinductivity beta-tricalcium phosphate crystal form; then, compounding with carboxymethylcellulose to prepare composite powder; mixing with deionized water for carrying out compression moulding, and drying to obtain a bracket material; subsequently, mixing bone morphogenetic protein, platelet-derived growth factors and phosphate buffer to prepare phosphate nutrient solution; then, dipping the bracket material in the phosphate nutrient solution for culture growth; finally, taking out, drying and sterilizing the bracket material to obtain the natural bone repairing material capable of accelerating osteanagenesis. The bone repairing material prepared with the preparation method has the advantages of high mechanical strength, good biocompatibility with the human body, good osteoinductive activity, good biodegradability and a wide application prospect, and bone defect repair can be quickly finished.

The invention discloses a handling method for inducing citrus bud mutation through NaN3. The method includes the following steps that NaN3 conditioning fluid is prepared; citrus one-to-two-year-old grafted seedlings are selected, the part, which is not lignified completely, of a branch to be handled is cut off, and all leaves and germinal buds of the reserved part of the branch are removed; all leaf axils of the reserved part of the branch are incised by a sharp blade; the branch to be handled is wrapped with absorbent paper by one to two layers, and the wrapped absorbent paper is wetted by the NaN3 conditioning fluid to be saturated through a liquid-moving machine and the like; the branch is covered with a preservative film until handling is completed, so that the conditioning fluid is prevented from being evaporated; the wrapped absorbent paper is uncovered, a plant is placed under normal culture conditions such as the outside of a room to grow, it can be observed that new shoots can germinate and grow from certain leaf axils after five days, and afterwards, variation character observation and statistics of leaf morphology and the like can be conducted. In the culture growth process after handling, the buds growing at the unhandled part of the plant need to be removed in time, in accordance with experimental result statistics, the mutagenesis effect is obvious, and application value can be achieved.

The invention relates to influence and an application method of different strains on hairy roots of ginseng, and belongs to the technical field of crop biology. The process comprises the following aspects: (1) explant treatment; (2) storage and activation of strains; (3) optimization of ginseng hairy root induction conditions; (4) acquisition and identification of ginseng hairy roots; (5) determination of the ginseng hairy root liquid, the solid culture growth amount and the content of total saponins; (6) establishment and condition optimization of a ginseng hairy root regeneration system; (7) study of physiological-biochemical characteristics in the hairy root regenerating plant pathway; and (8) histological observation and karyotype analysis on ginseng hairy roots and culture thereof. According to the influence and application method, ginseng is infected by agrobacterium rhizogene to induce hairy roots, a plurality of influence factors of hairy root induction and the hairy root regenerating plant process can be provided, a novel path is provided to germplasm resource storage and creative storage of ginseng, and a basis is established for ginseng breeding.

The invention relates to a capsella bursa-pastoris water culture nutrient solution. The capsella bursa-pastoris water culture nutrient solution comprises major elements, trace elements and water which are required for growth of capsella bursa-pastoris, can be used for replacing soil to provide water, fertilizer, air, heat and other growth factors for the capsella bursa-pastoris, and can meet the conditions required for water culture growth of the capsella bursa-pastoris. The capsella bursa-pastoris water culture nutrient solution is divided into a solution A and a solution B, wherein the solution A comprises the following main components: calcium nitrate tetrahydrate, potassium nitrate, ammonium hydrogen phosphate and magnesium sulfate heptahydrate; and the solution B comprises the following main components: ammonium nitrate, zinc sulfate heptahydrate, boric acid, copper sulfate pentahydrate, ethylenediaminetetraacetic acid disodium ferric salt, potassium iodide, aluminum chloride, borax, manganese sulfate, nickel chloride, sodium molybdate, citric acid, lead acetate, hydrogen selenide and sodium chloride. The capsella bursa-pastoris water culture nutrient solution has the advantages of complete nutritional components, simple preparation, high practicality and certain popularization and application values.

The invention discloses a summer pepper seedling culture growth regulating agent and a seedling strengthening cultivation method. The summer seedling culture growth regulating agent comprises the following components by mass concentration: 15 to 45 mg / L salicylic acid, 2.5 to 10 mg / L 5-aminolevulinic acid, 0.1 to 0.2 mg / L brassinolide, 0.25 to 1.0 mg / L forchlorfenuron, 25 to 100 mg / L paclobutrazoland 1000 to 2000 mg / L of potassium dihydrogen phosphate. The summer seedling culture process comprises: seedling culture matrix preparation, pretreatment, sowing and seedling strengthening regulation. According to the summer pepper seedling culture growth regulating agent and the seedling strengthening culture method, various plant growth regulating substances are reasonably compounded, so that the seedling strengthening effect of pepper seedlings in summer is improved to the maximum extent.

The invention discloses a quick culturing method of early fruit high-yield walnut trees. The method comprises: selecting good young trees to carry out fixed planting with a 4 - 6 m plant interval and 4 - 6 m row interval; in the second or third year after the young trees are planted, keeping three to four branches which are longer than 60 cm on the center trunk and culturing the three to four branches into first layer main branches; in the third or fourth year after the young trees are planted, selecting two to three branches above the first layer main branches and culturing the two to three branches into second layer main branches; in the fourth or fifth year after the young trees are planted, selecting two branches above the second layer main branches and culturing the two branches into third layer main branches; and at the same time, culturing two first level lateral branches on each first layer main branch, culturing two to three first level lateral branches on each second layer main branch, and culturing two first level lateral branches on each third layer main branch. Compared with conventional shaping trimming, the method shortens the tree culturing growth period by two to three years, so that high-yield tree structures can be cultured after planting for four to five years; and the yield is increased by 117%, and thus the method produces very good economical benefit.

The invention discloses a water-culture growth cabinet for plants. The cabinet comprises a box body, a field planting board weighing module, a culture liquid temperature control circulation and root-airing module, a lamp board height automatic adjustment mechanism and a main controller. According to the cabinet provided by the invention, through the culture liquid temperature control circulation and root-airing module, a culture liquid can be in a circular flow state, and temperature control and root airing functions are realized; through the field planting board weighing module, the weights of plants are periodically detected; and the lamp board height automatic adjustment mechanism and the weighing function of the field planting board weighing module are combined, the height of a lamp board is adjusted according to the plant weight data detected by the field planting board weighing module, and the height of the lamp board is suitable for the growth heights of the plants.

The invention discloses a controllable semi-open breeding cabin system. The controllable semi-open breeding cabin system comprises a breeding cabin, a water inlet mechanism, a filtering mechanism and a fish outlet pipe; the water inlet mechanism comprises a water inlet pipe and a plurality of high-pressure water inlet pipes; the water inlet pipe is located on one side of the upper portion of the breeding cabin, and the high-pressure water inlet pipes are distributed in the circumferential direction of the top of the breeding cabin; the tail ends of the high-pressure water inlet pipes are connected with a jet pipe extending into the breeding cabin; a filtering mechanism is arranged at the bottom of the breeding cabin, and the outlet end of the filtering mechanism is located in external seawater; and the fish outlet pipe is arranged on the cabin wall of the breeding cabin, and a gate valve capable of being opened and closed is installed on the fish outlet pipe. According to the controllable semi-open breeding cabin system, on the premise that the marine environment is not damaged, the optimal culture growth environment is provided for rare fishes by utilizing the marine self-purification capacity of the deep sea and the flow field controllable capacity of the culture cabin, and high energy consumption required by operation of a closed circulating water culture system is effectively reduced; Aand the damage to the marine environment caused by pollutants generated by open type culture is effectively reduced.

A method and a device for controlling etching waste liquid with low copper content by using algae. A combined control method by using algae growth and culture adsorption and algae powder adsorption. When concentration of a copper-containing etching waste liquid is higher than a copper concentration 50mg / L required by algae culture adsorption, first the copper-containing etching waste liquid is subjected to algae powder adsorption; the copper-containing etching waste liquid is treated by N algae powder adsorption and desorption chambers to lower the concentration to the copper concentration required by algae cultivation adsorption; and then the copper-containing etching waste liquid is subjected to algae culture growth and metabolism adsorption treatment, so that the copper concentration is lowered to less than 1.0mg / L to reach discharge requirements of copper-containing waste liquid. The invention can effectively treat copper-containing waste liquid with low concentration; and copper ions adsorbed in the algae can be desorbed, and copper in the waste liquid can be recovered to protect water environment.

The invention discloses a three-dimensional oxygen aeration system and method applicable to biological flocculation breeding method, and belongs to the technical field of aquaculture. The system comprises a culture pond, a perpendicular aeration oxygenation system, a jet flow oxygenation system and a pure oxygen aeration system. The perpendicular aeration oxygenation system comprises an aeration air inlet pipe and a dot-matrix aeration device. The jet flow oxygenation system comprises a water pump, a water supply pipe, a jet device and an air suction and inlet pipe. One end of the air suction and inlet pipe is connected with the water supply pipe, and the other end of the air suction and inlet pipe stretches out of the water surface. The pure oxygen aeration system comprises an oxygen conveying pipe and an adjustable air head. The oxygen conveying pipe is connected with the air suction and inlet pipe through the adjustable air head. In the culture preliminary stage, only the perpendicular aeration oxygenation system is started for aeration; in the culture middle period, the jet flow oxygenation system is started again for aeration, and in the culture later period, the pure oxygen aeration system is properly started for aeration according to the dissolved oxygen phenomenon. On the basis of different periods of biological flocculation culture and control requirement, the proper oxygenation mode is selected to be enabled, and water dissolved oxygen can be kept to meet the requirement for biological culture growth all the time.

The invention provides a grifola frondosa three-stage culture production method. The grifola frondosa three-stage culture production method comprises the steps of first-stage culture production, second-stage culture production, third-stage culture production, culture package production, inoculation and cultivation, bud forcing and mushroom cultivation. The third-stage culture production comprises the steps of obtaining of culture medium recipes, production of the culture media, bagging, sterilization, inoculation, cultivation of the culture and raising of the culture. The grifola frondosa three-stage culture production method is characterized in that the culture medium recipes include the wood strip culture medium recipe and the wheat grain culture medium recipe, and the production of the culture media comprises production of a wood strip culture medium and production of a wheat grain culture medium. Compared with a wood chip culture, the culture obtained through the grifola frondosa three-stage culture production method has the advantages that inoculation efficiency is 5-8 percent higher, the maximum culture growth distance is 50-100 percent shorter, the cultivation efficiency is 20-60 percent higher, each culture bag is consistent in mycelial age vertically, so that nutrients are released in a centralized mode, and the first-batch mushroom yield can be increased by 15-30 percent. Compared with liquid culture, the culture obtained through the grifola frondosa three-stage culture production method is applicable to existing culture production and detection, inoculation equipment and technicians in China, and can reduce the cultivation infection rate by 2-5 percent.

An air quality test unit for attachment to a vent of a HVAC system with air flow there through. The air quality test unit contains at least two substrate panels arranged in a v-shape form wherein at least one panel includes a sticky surface or a collection container. The air quality test unit is attachable to the vent by one or more clips. The sticky surfaces or collection container of the substrate panels capture airborne substances and contaminants emanating from the air flow of the HVAC system vent. A culture growth medium may be disposed in the collection container or on the sticky surface. The air quality test unit kit provides a user with an air quality test unit and process for shipping the unit to the laboratory for analysis.

The invention relates to a preparation method of an immobilized all-anthropogenic ECM coating matrix, first, extracellular matrix can be obtained from human umbilical cord mesenchymal cells by cell lysis method, and finally the extracellular matrix is immobilized by methanol. The matrix is an immobilized extracellular matrix which is all-anthropogenic and ready-to-use, can be saved for a long time and is suitable for long-term culture growth of human embryonic stem cells, and can be used for in vitro amplification of clinical grade human embryonic stem cells.

A method for improving quality of water spinach is used during soilless culture growth of the water spinach. The method comprises the steps that an organic carbon fertilizer is taken as a leaf fertilizer of the water spinach for a spraying treatment in a rainy season after culture of the water spinach. The organic carbon fertilizer is an organic carbon transparent solution obtained by processing of kitchen rubbish, wherein the content of organic carbon is 25-50 mg / L. Urea with the proportion of 0.1% is also contained in the organic carbon fertilizer. During the spraying, the organic carbon fertilizer shall be sprayed uniformly from top to bottom on the leaf surface of a water spinach plant; the spraying shall be carried out for one time every two to four days; and the spraying shall be carried out for four to five times in total. According to the method provided by the invention, the leaf surface spraying of the organic carbon fertilizer is performed on the soilless-cultured water spinach, so that the water spinach can be nourished during the growth; the problem about harms brought to the water spinach under a dim light stress situation can be relieved; the problem about the poor quality of the water-cultured water spinach under the dim light stress situation can be effectively solved; yield and taste of the water spinach can be improved; and adaptability and stress tolerance of the water spinach in the stress situation can also be enhanced.

The invention provides high-efficiency sulfate reducing bacteria (the bacteria is named Desulfotomaculum halophilum RETECH-SRB-II, the preservation unit is China Center for Type Culture Collection in Wuhan University, the preservation date is May 11th, 2007, and the preservation registration number is CCTCC NO of M207061) with wider growth temperature and pH applicability; the invention simultaneously provides a technique that can treat acid mine wastewater with the carbon and nitrogen source of straws. In the treating of the acid mine wastewater, the technique reaches the Cu<2+> removing rate of 89 to 97 percent, the Zn<2+> removing rate of 90 to 98 percent, the Fe<2+> removing rate of 91 to 99 percent, the Fe<3+> removing rate of 79 to 91 percent, and the As<3+> removing rate of 80 to 90 percent. The treated wastewater takes neutral pH value. The technique has the advantages that straws can be used as the carbon and nitrogen source required for culture growth, thus lowering the bacteria cultivation cost by 30 to 50 percent.

The embodiment of the present invention discloses a production method of thyme essential oil, in which the thyme aseptic seedlings obtained by germination of thyme seeds are subjected to adventitious bud induction culture and multiplication culture to obtain thyme tissue cultured seedlings, and then the thyme group is treated by steam distillation. The dry sample of the seedlings is processed to obtain the thyme essential oil, because the chitosan of a certain concentration is configured in the proliferation medium during the proliferation culture, thereby greatly improving the content of the thyme essential oil, making this production method suitable for industrialized production, the above-mentioned thyme The tissue culture seedling adopts plant in vitro culture technology, which saves valuable land resources, avoids environmental damage, and greatly shortens the production cycle of thyme essential oil, achieving huge economic and social benefits.

The invention discloses culture solution and a culture growth method of silver gray pearls. The culture growth method is characterized by comprising the following steps of: utilizing raw material ingredients of 1 to 5 parts of dry fish scale, 2 to 5 parts of ginkgo skin, 2 to 6 parts of actinolite, 2 to 6 parts of dry achyranthes bidentata, 1 to 6 parts of parasitism leaves, 1 to 5 parts of adenosine disodium triphosphate and the like and respectively preparing aqueous solutions of the raw material ingredients; firstly, soaking nuclei in mixed solution of the fish scale solution, the ginkgo skin solution, the actinolite solution and the achyranthes bidentata solution in a warm environment for 3 to 7h, taking out the nuclei and air-drying for 3 to 5h; then soaking the nuclei in the parasitism leaf solution for 3 to 5h, taking out the nuclei and air-drying for 2 to 3h; finally, after soaking the nuclei into 10 to 20ml of dilute solution of the adenosine disodium triphosphate, immediately taking out the nuclei, completing transplanting cultured objects into pearl shells in 1 to 3min and placing the pearl shells in which transplanting of the cultured objects is completed into a sea area to culture for 1 to 3 years so as to grow into the pearls. According to each obtained pearl, the surface is silver gray and is smooth; a pearl layer and the nucleus are tightly combined; pearl brightness is strong and soft; the color is bright; and the pearl is bright, smooth, gorgeous and noble.

The invention relates to a nitrosation-anaerobic ammonia oxidation treatment device for city sewage and a method. The device comprises a nitrosation reaction tank and an anaerobic ammonia oxidation reaction tank, wherein the nitrosation reaction tank is filled with vermiculite; nitrifying bacteria are in biofilm culturing growth on the surface of the vermiculite; the anaerobic ammonia oxidation reaction tank is filled with vermiculite; and anaerobic ammonium oxidation bacteria are in biofilm culturing growth on the surface of the vermiculite. By adopting the device provided by the invention, the vermiculite on which microorganisms are in biofilm culturing growth is arranged in the reaction tanks, so that treatment activity of the microorganisms upon wastewater can be effectively improved,and the denitrification performance of a nitrosation-Anammox process upon low ammonia nitrogen city sewage can be remarkably improved.

The invention relates to the technical field of culture media of desert alga, and provides a culture medium capable of promoting the growth of desert alga and a method for culturing the growth of thedesert alga by adopting the culture medium. In the culture media, soluble salt ions comprise Na+, K+ and Mg<2+>, and the concentration of the soluble salt ions is 10<-6>mM to 10<-2>mM. The method forculturing the growth of the desert alga comprises the following steps: inoculating activated desert alga cells to the culture medium of the desert alga, and carrying out static culture on the culturemedium under the light condition, wherein during static culture, a small amount of the culture solution is taken from the culture medium each day, when the number of cells of the desert alga or the optical density value at 540nm of the culture solution is measured to be stable, the culturing of the desert alga is completed. According to the technical scheme, the culture medium capable of promotingthe growth of the desert alga is obtained through allocation, so that an excellent method for culturing the growth of the desert alga is obtained, the number of the cells of the desert alga is improved by 30%-50% compared with the prior art, the yield of the desert alga is effectively improved, and the large-scale culture of the desert alga is facilitated.

The invention discloses a culture method for mushroom. The culture method comprises the following steps: constructing a culture greenhouse; preparing an inoculating bag; carrying out inoculation; carrying out greenhouse culture management; selecting and treating a culture land; carrying out mushroom accelerating breeding and management; carrying out mushroom growing management; harvesting the mushroom; and airing the mushroom. By preparing a breeding culture medium from 3-5 parts of saccharose, 5-8 parts of rice bran, 30-35 parts of miscellaneous wood chips, 10-15 parts of corncob powder, 10-15 parts of straw particles, 5-10 parts of clean water, 1-3 parts of amino acids, 3-5 parts of bagasse and 5-10 parts of eroded mountain soil, the culture growth speed is accelerated, the whole breeding period is shortened, the mushroom can be bred all the year round, the breeding period is short, the cost is low, the survival rate is high, and the output is high. The bred mushroom is rich in nutrient value, has the functions of improving immunity of body, delaying aging, preventing and resisting cancers, reducing blood pressure, blood fat and cholesterol, promoting digestion, treating constipation and the like, and is worth being popularized.

The invention discloses culture solution and a culture growth method of silver gray pearls. The culture growth method is characterized by comprising the following steps of: utilizing raw material ingredients of 1 to 5 parts of dry fish scale, 2 to 5 parts of ginkgo skin, 2 to 6 parts of actinolite, 2 to 6 parts of dry achyranthes bidentata, 1 to 6 parts of parasitism leaves, 1 to 5 parts of adenosine disodium triphosphate and the like and respectively preparing aqueous solutions of the raw material ingredients; firstly, soaking nuclei in mixed solution of the fish scale solution, the ginkgo skin solution, the actinolite solution and the achyranthes bidentata solution in a warm environment for 3 to 7h, taking out the nuclei and air-drying for 3 to 5h; then soaking the nuclei in the parasitism leaf solution for 3 to 5h, taking out the nuclei and air-drying for 2 to 3h; finally, after soaking the nuclei into 10 to 20ml of dilute solution of the adenosine disodium triphosphate, immediately taking out the nuclei, completing transplanting cultured objects into pearl shells in 1 to 3min and placing the pearl shells in which transplanting of the cultured objects is completed into a sea area to culture for 1 to 3 years so as to grow into the pearls. According to each obtained pearl, the surface is silver gray and is smooth; a pearl layer and the nucleus are tightly combined; pearl brightness is strong and soft; the color is bright; and the pearl is bright, smooth, gorgeous and noble.