Culture medium for hepatoma organoid cell sphere culture

Abstract

The invention discloses a culture medium for hepatoma organ cell sphere culture. The culture medium is composed of a basic culture medium, 10-25 ng/mL of an epidermal growth factor, 10-25 ng/mL of a fibroblast growth factor, 0.5-2 ng/mL of a cell growth factor, 1-3 ng/mL of an insulin growth factor, 1 mmol/L of sodium pyruvate, 2 mmol/L of glutamine, 100 U/mL of penicillin, 100 [mu]g/mL of streptomycin and 10% by volume of serum. Aiming at the culture growth characteristics of liver cancer tissue-derived cells, the culture medium provided by the invention is simple and reasonable in components, rich in nutrition and moderate in formula price; and hepatoma organ cell spheres cultured by the culture medium can effectively and stably grow for a long time, are closely gathered, have smooth andround surfaces and clear boundaries, is free of disintegration phenomenon, and can be significantly improved in cell activity, specific functions and metabolic functions.

Description

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AI Technical Summary

Benefits of technology

This patented technology provides various factors that help culture hepatoma tissue (LUT) cells efficiently over longer periods without losing their original shape or quality. These factors include certain proteins like albumin, transferrin, bile acid binding protein, growth factor, insulin receptor substrates, hormones such as estrogen, progesterone, etc., along with other substances found naturally within it called lignans. By selecting these ingredients carefully at different ratios, they create special structures made up of many small particles that make them easier to work with during experiments on animals than traditional methods involving living organisms.

Problems solved by technology

The technical problem addressed in this patents relating to developing optimized hepatocarcinoma medicine treatment strategies involves finding ways to improve their effectiveness while minimizing side effects associated with chemotherapy treatments. Current approaches involve either traditional monolayer cell culture techniques like bile duct carcinostomy or autophagy, or various advanced technologies including xenograft models, hollow fiber modelings, and immune epidermis scaffolds. These advancements aim towards better understanding how normal and diseased livers develop into multiple types of brain structures called cerebrals, providing valuable insights regarding potential future therapies targeting these areas.

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