Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros

Abstract

The invention relates to a tissue culture and fast-propagation technology for virus-free plantlets of red bud taros. Healthy and strong corms without damage by diseases and insects are selected, sterilized and disinfected; corm tips of 0.2mm are picked out and then inoculated into the culture medium for differentiated culture; cluster buds are cultured, the top ends of the cluster buds are cut down and then the cluster buds are placed in a proliferation culture medium for fast-propagation; and when the leaves of the cluster buds spread, the cluster buds are placed in a rooting culture medium for strong plantlet rooting culture and later transferred to plantlet hardening. As for the method, a virus eradication technology is adopted twice in propagation to pick out the corm tips of the test tube plantlets and the virus eradication effect is improved. In the preparation of the culture medium, the formula is optimized, thus being beneficial to the growth of the test tube plantlets. The defects of large usage quantity of seeds and low cost when the corms are used as propagating materials in tradition are overcome; the culture growth conditions can be controlled artificially, therefore the method is free from the influence of natural conditions; and the usage quantity of materials is less, the culture materials are economical, the growth cycle is short, the propagation coefficient is high, the production cost is lowered, the variety property is maintained, the yield and the quality are improved, and industrial plantlet culture and industrialized production can be realized.

Description

technical field [0001] The invention relates to a plant tissue culture and detoxification and rapid propagation technology, specifically, detoxification with the stem tip meristem of red bud taro, rapid propagation with clustered buds, and the proportioning composition of the culture medium used for breeding . Background technique [0002] Red bud taro ( Clocasia escalozta Schott ) is a perennial herbaceous plant belonging to the Araceae family. It is usually cultivated as an annual. It is planted all over my country, with the most in the Pearl River Basin and Taiwan Province, followed by the Yangtze River and Huaihe River Basins, and less in the north. [0003] Red bud taro is also called taro and red-eyed taro. Its bulbs are rich in starch, protein, mucus juice and various vitamins and minerals. Due to the small starch particles, its digestibility can reach 98.8%. Red bud taro is not only a vegetable, but also a grain. It can be cooked, and can also be used as a raw mate...

AI Technical Summary

Problems solved by technology

However, there is no virus-free vaccine on the market so far, which limits the development of production