A kind of method for rapid propagation of Radix chinensis tissue culture
A technology of tissue culture and lotus, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of reduced yield, consumption of large tubers, low germination rate of seeds, etc., to improve yield and quality, and promote industrial development The effect of chemical production and ensuring the purity of seedlings
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[0016] A kind of method for the fast propagation of the lotus tissue culture, concrete steps are:
[0017] Take the tip of the young tuber of the lotus root as the explant, after disinfection treatment, strip off the growth point of 0.2 mm in size under the vertical microscope, transfer it to the adventitious bud induction medium for cultivation, and transfer it when the adventitious bud grows to 1.5 cm. Into the strong seedling medium, the culture conditions of test tube seedlings are: temperature 25°C, light 1500Lx / 12h.d, pH 6.0;
[0018] When the test-tube seedling grows to 3cm, transfer it to the rooting medium. When the root length is 5cm, harden the strong rooting seedling, remove the rooting bottle seedling to the culture rack, place it at room temperature and under natural light to adapt to the natural environment, and exercise for 5 days , and then open the bottle cap to clean the root agar, transplant it into the vermiculite substrate, pour the fixed root water thoro...
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