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Asexual Propagation Method of Artemisia annua Based on Maintaining the Species of Female Parents of Fine Varieties

A technique of asexual reproduction and Artemisia annua, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of difficult operation and achieve the effects of less difficult management, maintenance of species, and high rooting survival rate

Active Publication Date: 2017-04-12
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent (200810069248.8) provides a method of asexual reproduction of Artemisia annua based on maintaining the female parent species. This method requires branches with a length of 15-20 cm. At the same time, it needs to be covered with plastic film and artificially controlled temperature and humidity. The operation is extremely difficult

Method used

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  • Asexual Propagation Method of Artemisia annua Based on Maintaining the Species of Female Parents of Fine Varieties
  • Asexual Propagation Method of Artemisia annua Based on Maintaining the Species of Female Parents of Fine Varieties
  • Asexual Propagation Method of Artemisia annua Based on Maintaining the Species of Female Parents of Fine Varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Investigation of the Effects of Different Cutting Substrate Conditions on Rooting of Artemisia annua Cuttings

[0022] Collect Artemisia annua plants with an artemisinin content higher than 0.9% in Youyang, Chongqing, and collect cuttings in July-August of that year. Cuttings use side branches (randomly selected) from the upper, middle and base of the plant, 3 nodes / branch, and remove the base 2 Nodal leaves, leaving half of the upper node, inserted obliquely into the substrate, and 2 nodes into the soil; 20 branches / treatment.

[0023] In this experiment, the mixture of river sand and humus was used as the substrate instead of soil or perlite, because it was found in the previous pre-experiment that the base of the cuttings was easy to rot when soil was used as the substrate, while the perlite The water retention effect is not as good as river sand, which has good water permeability and air permeability. However, river sand as a substrate is easy to dry, so ...

Embodiment 2

[0027] Example 2 Investigation of the Effects of Different Cutting Temperature Conditions on Rooting of Artemisia annua Cuttings

[0028] Collect Artemisia annua plants with an artemisinin content higher than 0.9% in Youyang, Chongqing, and collect cuttings in July-August of that year. Cuttings use side branches (randomly selected) from the upper, middle and base of the plant, 3 nodes / branch, and remove the base 2 Nodal leaves, leaving half of the upper node, inserted obliquely into the substrate, and 2 nodes into the soil; 20 branches / treatment. In order to realize the temperature control of the large environment, the experimental plots were selected in the Youyang area at an altitude of 430m (the ambient temperature in July and August is 30-37°C, and the average temperature is 34°C), and an altitude of 890m (the ambient temperature in July and August is 24- 28°C, the average temperature is 26°C), and the altitude is 1320m (the ambient temperature in July and August is 15-23°...

Embodiment 3

[0054] Example 3 Effects of Different Cutting Seasons on the Survival Rate of Cuttings

[0055] Collect Artemisia annua plants with an artemisinin content higher than 0.9% in the Youyang area of ​​Chongqing, collect cuttings in May-June of that year, use side branches (randomly selected) from the upper, middle and base of the plant for cuttings, and make cuttings as treatment A; Gather cuttings in July-August, and cuttings use the side branches (selected at random) of the top, middle and base of the plant to make cuttings as processing B; gather cuttings in September-October that year, use the side branches (selected at random) of the top, middle and base of the plant for cuttings. ), making cuttings as processing C. Leave 3 nodes for each cutting, remove 2 leaves at the base, leave half a leaf at the upper node, insert obliquely into the substrate, and 2 nodes into the soil. For each treatment, 3 groups were paralleled, with 20 branches in each group; the ambient temperature...

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Abstract

The invention provides an artemisia annua vegetative propagation method on the basis of keeping the improved-variety maternal character. Artemisia annua is vegetatively propagated, selfing propagation of artemisia annua is avoided, and then vegetative propagation (passage) is carried out once again before maternal artemisia annua blooms. The method is technically characterized in that the maternal plant of the artemisia annua is propagated in a cuttage mode, propagation and passage are carried out once again in a cuttage mode before the plant blooms, the hereditary character of maternal vegetative propagation materials is kept, and maternal character degeneration caused by sexual propagation is avoided.

Description

technical field [0001] The invention relates to a method for asexual propagation of plants, and more specifically relates to a method for asexual propagation of Artemisia annua cuttings based on maintaining the pedigree of the female parent. [0002] technical background [0003] Artemisia annua (Artemisia annua L.), also known as Artemisia annua, is an annual herb of the genus Artemisia in the family Asteraceae. Its stems and leaves are used as medicine. Contains the special ingredient artemisinin, which is not only a special medicine against malaria, but also has certain curative effect on hepatitis, schistosomiasis and cancer. [0004] At present, the only source of medicinal artemisinin in the world is extracted from the leaves of Artemisia annua. Although natural Artemisia annua is widely distributed, the content of artemisinin is very low, and it is easily affected by factors such as variety, geographical environment, collection period, collection location, temperature...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00
Inventor 伍晓丽崔广林李隆云
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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