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Culture medium capable of promoting growth of desert alga and method for culturing growth of desert alga by adopting culture medium

A growth medium and technology of desert algae, which is applied in the field of promoting the growth medium of desert algae and cultivating the growth of desert algae, can solve the problems of weakened photosynthesis, low number of desert algae cells, and low yield of desert algae, so as to increase production, Effects that are conducive to large-scale cultivation

Inactive Publication Date: 2018-06-01
新疆拓必达科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the autotrophic culture technology of desert algae has been mature, but with the continuous proliferation of cells, the light entering the culture is blocked, resulting in weakened photosynthesis, which limits the further increase of its biomass, and the number of desert algae cells obtained Low, so that the yield of desert algae is not high

Method used

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  • Culture medium capable of promoting growth of desert alga and method for culturing growth of desert alga by adopting culture medium

Examples

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Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: The medium for promoting the growth of desert algae includes soluble salt ion Na + 、K + and Mg 2+ , the concentration of soluble salt ions is 10 -6 mM to 10 -2 mM, promote the growth medium of desert algae to obtain according to the following method: first prepare the stock solution that promotes the growth medium of desert algae, when the soluble salt ion concentration is 10 in the stock solution -6 mM to 10 -2 At mM, the medium for promoting the growth of desert algae was obtained.

[0019] In the prior art, the soluble salt ion Na in the growth medium of desert algae + 、K + and Mg 2+的 Poor concentration preparation ratio will inhibit the rapid growth of desert algae cells inoculated in the growth medium of desert algae, thereby reducing the number of desert algae and affecting the yield of desert algae.

[0020] The present invention promotes the growth medium of desert algae, and provides the optimal concentration of required soluble salt ions ...

Embodiment 2

[0021] Embodiment 2: the medium for promoting the growth of desert algae includes soluble salt ion Na + 、K + and Mg 2+ , the concentration of soluble salt ions is 10 -6 mM or 10 -2 mM, promote the growth medium of desert algae to obtain according to the following method: first prepare the stock solution that promotes the growth medium of desert algae, when the soluble salt ion concentration is 10 in the stock solution -6 mM or 10 -2 At mM, the medium for promoting the growth of desert algae was obtained.

Embodiment 3

[0022] Example 3: As an optimization of the above examples, the stock solution of the growth medium for promoting desert algae was prepared according to the following steps: the first step, the first stock solution was prepared under high temperature and sterilized conditions: the NH 4 CL, MgSO 4 •7H 2 O, MgSO 4 , CaCl 2 •H 2 O, CaCl 2 Mix and dissolve with deionized water to obtain the first stock solution, wherein, every 1L of the first stock solution contains 0.4g NH 4 CL, 0.156 gMgSO 4 •7H 2 O, 0.078g MgSO 4 , 0.05g CaCl 2 •H 2 O and 0.043g CaCl 2 ; Prepare the second stock solution under high temperature and sterilized conditions: K 2 HPO3 •3H 2 O, K 2 HPO 3 、KH 2 PO 4 Mix and dissolve with deionized water and filter to obtain the second stock solution, wherein, every 1L of the second stock solution contains 0.142g K 2 HPO 4 •3H 2 O, 0.11g K 2 HPO 3 , 0.056g KH 2 PO 4 ; Prepare the third stock solution under high temperature and sterilized condition...

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Abstract

The invention relates to the technical field of culture media of desert alga, and provides a culture medium capable of promoting the growth of desert alga and a method for culturing the growth of thedesert alga by adopting the culture medium. In the culture media, soluble salt ions comprise Na+, K+ and Mg<2+>, and the concentration of the soluble salt ions is 10<-6>mM to 10<-2>mM. The method forculturing the growth of the desert alga comprises the following steps: inoculating activated desert alga cells to the culture medium of the desert alga, and carrying out static culture on the culturemedium under the light condition, wherein during static culture, a small amount of the culture solution is taken from the culture medium each day, when the number of cells of the desert alga or the optical density value at 540nm of the culture solution is measured to be stable, the culturing of the desert alga is completed. According to the technical scheme, the culture medium capable of promotingthe growth of the desert alga is obtained through allocation, so that an excellent method for culturing the growth of the desert alga is obtained, the number of the cells of the desert alga is improved by 30%-50% compared with the prior art, the yield of the desert alga is effectively improved, and the large-scale culture of the desert alga is facilitated.

Description

technical field [0001] The invention relates to the technical field of desert algae culture medium, and relates to a medium for promoting the growth of desert algae and a method for cultivating the growth of desert algae. Background technique [0002] Desert algae refers to a type of algae isolated from desert soil and artificially cultivated purely. Because its habitat is a harsh desert environment, people are accustomed to collectively refer to this type of algae as desert polar algae. Biological and nutritional studies on desert algae cells have found that such cells not only have unique nutritional characteristics, but also have a very ancient developmental status in biological evolution, which fully demonstrates that the Taklamakan Desert was once a vast ocean. With the disappearance of the ocean, the seaweed gradually evolved into today's desert algae. Desert algae is a specific ecological group of terrestrial algae, mainly referring to algal organisms that grow in de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G33/00C12R1/89
CPCA01G33/00C12N1/20Y02A40/80
Inventor 艾山江艾萨江·图合提阿不都克力木·白克尔古丽山·买买提
Owner 新疆拓必达科技发展有限公司
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