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Fungus strain for degrading polyurethane plastic as well as culture method and application thereof

A technology of polyurethane and bacterial strains, which is applied in the field of bacterial strain culture and fungal strains, and can solve problems such as biodegradation of PU that have not been found

Active Publication Date: 2020-11-17
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no method has been found to biodegrade PU with high rate (e.g., in less than 30 days) and large-scale production

Method used

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  • Fungus strain for degrading polyurethane plastic as well as culture method and application thereof
  • Fungus strain for degrading polyurethane plastic as well as culture method and application thereof
  • Fungus strain for degrading polyurethane plastic as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Isolation and screening of fungal strain A.flavus G10:

[0036] S110. Isolating a fungal strain from the gut of a cricket.

[0037] Specifically, the cricket may be "Gryllus bimaculatus" cricket. Specifically, fungal strains were isolated from the gut of crickets.

[0038] S120, using PU as the sole carbon source, culturing the fungal strain in a liquid medium to obtain a culture solution;

[0039] Specifically, by dissolving each segment of the cricket gut in sterile saline solution and incubating the gut in a liquid medium.

[0040] Among them, the preparation method of the liquid medium is: add 0.7g KH to 1000ml deionized water 2 PO4, 7.7gK 2 HPO 4 , 0.7g MgSO 4 ·7H 2 0, 1.0gNH 4 NO 3 , 0.005g NaCl, 0.002g FeSO 4 ·7H 2 O, 0.002gZnSO 4 ·7H 2 O, 0.001gxx. Liquid media were sterilized by autoclaving at 121°C for 15 minutes before use.

[0041] S130. Dilute the culture solution containing the fungal strain and spread it on solidified nutrient ag...

Embodiment 2

[0045] Example 2: Identification of fungi

[0046] DNA from degrading fungal strains was isolated at room temperature (25-28°C), purified and plated on malt extract agar (MEA) plates. Morphological characterization was performed by light microscopy and stereo electron microscopy. The PU-degrading fungus isolated by molecular morphology and phylogenetic analysis was identified as Aspergillus flavus G10.

[0047] Specifically, the identification method is: morphological characterization by optical microscope and stereo electron microscope. In internal transcribed spacer (ITS5 / ITS4), large subunit (LR0R / LR5), RNA polymerase II second largest subunit (fRPB2-5f / fRPB2-7cR), calmodulin (CAL-228F / CAL2Rd) and β- for molecular identification. Tubulin (T1 / T2) sequence method. The ITS sequence data generated in this study were subjected to BLAST searches in GenBank's nucleotide database (www http: / / blast.ncbi.nlm.nih.gov / ) to identify their most likely closely related taxa. Single-ge...

Embodiment 3

[0048] Embodiment 3: The method for degrading polyurethane plastics using fungal strain A.flavus G10:

[0049] Cultivation conditions: The conditions in the incubator were maintained at a temperature of 24±2°C, a relative humidity of 75±2%, and a light ratio of 8:10. 300 crickets were cultivated. There were 6 small incubators in one incubator. There are 50 crickets in an incubator.

[0050] Among them, the control group: 150 healthy adult crickets were raised under the control conditions that the temperature was 24±2°C, the relative humidity was 75±2%, and the light ratio was 8:10, and fed with wheat bran, wheat germ and yeast powder. The ratio is 10:3:1 respectively. Add 0.2% of the total amount of vitamin powder and water in addition.

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Abstract

The invention provides a fungus strain A.flavus G10 for degrading polyurethane plastic. The microbial preservation number of the fungus strain A.flavus G10 is GDMCC 60537. The invention also providesa method for culturing the fungus strain A.flavus G10. The method comprises the following steps that the fungus strain is separated from the intestinal tract of a cricket; PU serves as a unique carbonsource, the fungus strain is cultured in a liquid culture medium to obtain a culture solution; the culture solution is diluted and coated on a solidified nutrient agar culture medium containing tetracycline antibiotics and a potato dextrose agar culture medium to obtain a culture growth substance; and the culture growth substance is subcultured on fresh plates at 30 DEG C until a single fungus strain is obtained on each plate. The fungus strain A.flavus G10 is high in speed of degrading the polyurethane plastic.

Description

technical field [0001] The invention relates to the field of biodegradation, in particular to a fungal strain for degrading polyurethane plastics, strain culture and application thereof. Background technique [0002] Plastic is a versatile material that is used in almost every aspect of our daily lives. Global per capita consumption of synthetic plastics is about 38 kg per year. From 1950 to 2015, about 91.5 million tons of plastic have been produced, resulting in about 69.45 million tons of plastic waste on the Earth’s surface (Gyer et al., 2015). A small fraction of the plastic waste generated can be recycled (9%) and incinerated (12%), with around 79% still accumulating in landfills and other surface and marine environments (Gyer et al., 2015). It is estimated that plastic waste accounts for 13% of the total annual municipal solid waste produced in the US at 243 million tons (EPA report 2009, US). In Europe, discarded plastics account for 15-25% of the 220 million soli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C08J11/10C12R1/67C08L75/04
CPCC12N1/14C12N1/02C08J11/105C08J2375/04C12R2001/67C12N1/145Y02W30/62C12R2001/645
Inventor 赛隆·可汗许建初桂恒赛迪亚·娜迪尔彼得·莫蒂默叶磊
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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