Handling method for inducing citrus bud mutation through NaN3

Abstract

The invention discloses a handling method for inducing citrus bud mutation through NaN3. The method includes the following steps that NaN3 conditioning fluid is prepared; citrus one-to-two-year-old grafted seedlings are selected, the part, which is not lignified completely, of a branch to be handled is cut off, and all leaves and germinal buds of the reserved part of the branch are removed; all leaf axils of the reserved part of the branch are incised by a sharp blade; the branch to be handled is wrapped with absorbent paper by one to two layers, and the wrapped absorbent paper is wetted by the NaN3 conditioning fluid to be saturated through a liquid-moving machine and the like; the branch is covered with a preservative film until handling is completed, so that the conditioning fluid is prevented from being evaporated; the wrapped absorbent paper is uncovered, a plant is placed under normal culture conditions such as the outside of a room to grow, it can be observed that new shoots can germinate and grow from certain leaf axils after five days, and afterwards, variation character observation and statistics of leaf morphology and the like can be conducted. In the culture growth process after handling, the buds growing at the unhandled part of the plant need to be removed in time, in accordance with experimental result statistics, the mutagenesis effect is obvious, and application value can be achieved.

Description

technical field [0001] The invention belongs to the technical field of biotechnology research and development, and relates to a treatment method for sodium azide-induced bud change of citrus. Background technique [0002] Compared with other chemical mutagens, sodium azide (NaN 3 ) has the advantages of no residual toxicity and relatively safe use, but so far there has been no research report on the method of using sodium azide to mutate citrus axillary buds. Sodium azide has been applied in the breeding research of plants such as barley, rice, sunflower, soybean, flower and apple, and some beneficial mutated plant materials have been obtained; There are following defects in the processing method of inducing citrus bud change: [0003] 1. The concentration (reagent preparation) and treatment time of sodium azide to effectively trigger citrus budding are not clear [0004] So far, there is no research report on how to treat citrus plants with sodium azide to effectively ca...

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Problems solved by technology

[0007] In order to overcome the defects existing in the prior art, the present invention proposes a kind of processing method that sodium azide induces citrus bud change, and this method clearly causes the concentration (reagent preparation) and processing time etc. that sodium azide effectively causes citrus axillary bud variation conditions, and to develop a simple and effective chemical mutagenesis treatment method for infecting citrus axillary buds, which can be applied to citrus bud mutation selection, and solve the problems that the natural variation rate of citrus is small and cannot actively carry out large-scale citrus resource innovation.

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