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Ploidy mutagenesis method of brier grape buds covered by propyzamide

A technology of pentoxychlor and thorny grapes, applied in the field of ploidy mutagenesis, to achieve the effects of increased probability, improved mutagenic effect, and vigorous cell division

Active Publication Date: 2016-09-21
HUNAN BIOLOGICAL & ELECTROMECHANICAL POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no successful report of applying pentynchlor to Prickly Grapes and inducing the chromosome doubling of Prickly Grapes. Ploidy induction, providing excellent germplasm resources and new lines for obtaining polyploid new varieties of thorny grapes with large grains, high quality, few seeds or no seeds

Method used

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  • Ploidy mutagenesis method of brier grape buds covered by propyzamide
  • Ploidy mutagenesis method of brier grape buds covered by propyzamide
  • Ploidy mutagenesis method of brier grape buds covered by propyzamide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of test material and fenpyramide agar paste

[0024] On March 21, 2014, 12 branches and tendrils with approximately the same growth, 15 buds (many side buds were erased) and already in an active state were selected from the thorn grape plants, and the selected branches were flattened and bound.

[0025] Preparation of Pennyclad Agar Paste (Cultivation Medium): Penpyrald is formulated into a mother solution with a concentration of 0.05%, and then diluted to form Pennylacet agar with a concentration of 0.003%, 0.004%, 0.005%, 0.006%, and 0.007%. clodinafil solution. The agar (10%) was fully dissolved into the above-mentioned pentynclochlor solutions and clear water to prepare respectively pentynchlor agar pastes of various concentrations and clear water control agar pastes packed in waterproof capsules.

[0026] Approach

[0027] The test is carried out by the hood method. Take clear water agar paste cover as a control, 3 treatments; 2 × 15 = 30 buds on vin...

Embodiment 2

[0066] This embodiment also provides the ploidy mutagenic effect test of the thorny grape buds at different shroud times. The test results showed that the mutagenesis rate of thorny grape buds was over 60% when treated with 0.005% pentynchlor for 6 days, and the ploidy mutagenesis effect was still the best; when the sheathing time was prolonged, the germination rate decreased significantly.

[0067] Table 5 Mutagenic effect of different shroud time on the ploidy of growing buds of thorny grapes

[0068]

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Abstract

The invention discloses a ploidy mutagenesis method of brier grape buds covered by propyzamide. The method comprises the following steps: (1) dissolving propyzamide in dimethyl sulfoxide, and diluting with water till the mass concentration of the propyzamide is 0.005%-0.007% to obtain a propyzamide solution; and (2) preparing the propyzamide solution into propyzamide agar paste, covering the brier grape buds in the budding growth state with the propyzamide agar past for 5-7 days to complete ploidy mutagenesis of the brier grape buds. Through a covering method, the ploidy mutagenesis method induces the brier grape buds in the budding growth state to successfully obtain brier grape tetraploids; the propyzamide is used as a main reagent; when the concentration of the propyzamide solution applied to the brier grape buds is 0.005%, an optimal mutagenesis effect is achieved; the mutagenesis rate reaches 60% or above.

Description

technical field [0001] The invention relates to a method for ploidy mutagenesis of fenpyrald-covered grape buds. Background technique [0002] The thorny grape (V. davidii. Foex) belongs to the East Asian population, 2n=2x=38, and is one of the main wild grapes in southern my country. It is mainly distributed in the subtropical warm and humid rain forests and mountains in the south of the Yangtze River, and can better adapt to high temperature and humidity conditions. It has good resistance to common diseases of grapes such as white rot, anthracnose, black pox, etc., and has strong adaptability. This unique performance has drawn a broad prospect for the pollution-free planting of grapes in the south, and it is also a good example for grape moisture-resistant rootstocks and Excellent resources and materials are provided for the selection and breeding of new varieties. The new vines, young leaves and fruits of the thorny grape are very colorful, and the ornamental effect and va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 蔡冬元林继华刘芳欧阳英徐一兰邓建平
Owner HUNAN BIOLOGICAL & ELECTROMECHANICAL POLYTECHNIC
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