Tobacco solanesol mutant creation method and application

A technology for mutant and solanesol, which is applied in the field of creation and application of tobacco solanesol mutants, can solve the problems such as difficulty in obtaining potato leaves and insufficient planting area, and achieves the effects of remarkable mutagenesis effect and simple method.

Inactive Publication Date: 2016-12-07
TOBACCO RES INST CHIN AGRI SCI ACAD
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Problems solved by technology

The content of solanesol in potato leaves is about 1.5%, but the planting area is not wide enough, a large amount of potato leaves are not easy to obtain, and the extraction of solanesol is limited by raw materials

Method used

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  • Tobacco solanesol mutant creation method and application
  • Tobacco solanesol mutant creation method and application

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Embodiment 1

[0021] (1) Use 0.01 mol / L phosphate buffer solution (Na2HPO4·12H2O-NaH2PO4·H2O) at pH 6.5 to configure an EMS solution with a mass volume ratio of 0.6%. Soak the seeds of the tobacco variety "Zhongyan 100" with prepared EMS at room temperature, and shake in a shaker (110rpm / min) for 16 hours. The treated seeds were rinsed with phosphate buffer solution for 3 times, and washed continuously with a large amount of distilled water to obtain EMS-induced tobacco M1 generation seeds.

[0022] (2) The seeds of the M1 generation were transplanted to the field two months after sowing, and the M2 generation seeds were harvested by bagging and self-crossing after a single plant matured; One plant was selected to take 3 leaves in the middle, and the M3 generation seeds of the plant were harvested.

[0023] (3) The middle fresh tobacco leaves of the M2 generation were roasted and ground into tobacco powder, and the total solanesol content in the tobacco leaves was determined by liquid chro...

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Abstract

The invention discloses a tobacco solanesol mutant creation method and application. The preparation method comprises the following steps that 1, a sthyl methyl sulfonate solution is prepared, and EMS mutagenesis tobacco M1 generation seeds are obtained; 2, the M1 generation seeds are sown, and selfing M2 generation seeds are obtained; 3, the M2 generation seeds are sown, a liquid chromatogram is used for determining the total solanesol content of tobacoo in the middle of an M2 generation single-plant mature period, and solanesol mutant plants are identified and screened. The mutant M3 generation strain obtained through the method is used for determining mutant smoke harmful ingredients, the content of solanesol is analyzed, and the significant positive correlation is analyzed. According to the tobacco solanesol mutant preparation and screening method, the obtained mutation rate is high, the mutant solanesol content changing amount is remarkable, and the important role is brought into play in tobacco breed improvement and functional component utilization.

Description

technical field [0001] The present invention relates to a preparation, screening method and application of a tobacco solanesol mutant, in particular to a method for preparing a tobacco solanesol mutant using a chemical mutagen EMS and the use of the mutant in the improvement of tobacco varieties and the utilization of functional components Applications. Background technique [0002] Solanesol is an important pharmaceutical intermediate, an irreplaceable component of ubiquinone drug intermediates, the side chain of synthetic vitamin K and coenzyme Q10, and the synthesis of anti-ulcer drugs, anti-cancer drugs, and drugs for the treatment of cardiovascular and cerebrovascular diseases , anti-HIV drugs, antihypertensive drugs raw materials. Due to its special molecular structure, solanesol is a drug intermediate that has attracted much attention and is in great demand in the pharmaceutical industry. It is difficult to synthesize solanesol, but its content is relatively high in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 龚达平陈明丽刘贯山闫宁刘艳华
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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