Phaffia rhodozyma strain with high yield of astaxanthin and breeding method and application of phaffia rhodozyma strain

Abstract

The invention discloses a phaffia rhodozyma strain for high yield of astaxanthin, which is a Phaffiarhodozyma LUCNOVA001 strain, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M20211124, has the preservation date of September 1, 2021, and is named as PhaffiarhodozymaLUCNOVA001 in taxonomy; the strain is obtained by taking Phaffiarhodozyma as a starting production strain and carrying out ARTP mutagenesis and [beta]-ionone screening, and the breeding method comprises the following steps: (1) activating the strain; (2) preparing bacterial liquid in a logarithmic phase; (3) preparing a bacterial suspension; (4) carrying out ARTP mutagenesis; (5) screening the [beta]-violet ketone; (6) secondary screening of strains; and (7) performing continuous passage. The astaxanthin yield and content of the strain and the corresponding biomass meet the requirements of industrial production, and the strain has a great application prospect.

Description

technical field [0001] The invention relates to the technical field of strain breeding, and more specifically relates to a Phaffia rhodozyme strain with high astaxanthin production, a breeding method and application thereof. Background technique [0002] Astaxanthin, also known as astaxanthin, astaxanthin, lobster shell pigment, etc., is naturally purple-red and widely exists in the biological world, especially in crustaceans, salmon, rainbow trout, etc., and is their main component. Pigment (1. Johnson E A, AnG H. Anstaxanthin from microbial sources. Critical Reviews in Biotechnology, 1991.11: 297-326; 2. Torrissen O J, Christiansen R. Requirements for carotenoidsin fish diets. J Appl Ichthyol., 1995.11: 225-230 3. Pharmaceuticals M. Aquaxan TMHD algal meal use in aquaculture diets: Enhancing nutritional performance and pigmentation. Inc. Technical Report TR, 1999.2102.001). [0003] The chemical name of astaxanthin is 3,3′-dihydroxy-4,4′-diketone-β,β′-carotene, and the mo...

AI Technical Summary

Problems solved by technology

Among them, the price of chemically synthesized astaxanthin is relatively cheap, and it still has certain competitiveness in feed additives, but its structure is mostly cis, and the animal utilization rate is low. Threat; For countries with developed shrimp and crab processing industries, crustacean waste is a good source of astaxanthin, but its astaxanthin content is very low, organic solvent pollution in the extraction process, low product purity and other problems It limits its further development; the content of astaxanthin in Haematococcus pluvialis generally accounts for 0.2%-2% of dry cell weight, up to 4%. It is an excellent source of astaxanthin, so it has been widely studied and application, but due to the extremely harsh growth conditions of Haematococcus pluvialis, high requirements on water quality, environment and light, as well as factors such as long cycle and large floor area, its large-scale production is relatively difficult; Phaffia rhodozyme It is the strain with the second highest content of astaxanthin in microorganisms. Due to its advantages such as simple cultivation method, short cycle and high astaxanthin activity, people have gradually shifted their attention from Haematococcus pluvialis to P. pluvialis in the past two decades. yeast on