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Method for mutating flavomycin producing bacteria by using high-voltage corona electric field and high-voltage corona electric field device thereof

A technology of high-voltage corona and flavomycin, applied in the direction of stress-stimulated microbial growth, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy

Inactive Publication Date: 2012-10-03
那日
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  • Abstract
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  • Application Information

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Problems solved by technology

And the method that utilizes high-voltage corona electric field to carry out mutagenesis to microorganism has not been reported yet

Method used

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  • Method for mutating flavomycin producing bacteria by using high-voltage corona electric field and high-voltage corona electric field device thereof
  • Method for mutating flavomycin producing bacteria by using high-voltage corona electric field and high-voltage corona electric field device thereof
  • Method for mutating flavomycin producing bacteria by using high-voltage corona electric field and high-voltage corona electric field device thereof

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Embodiment Construction

[0014] The present invention will be described in detail below with reference to specific embodiments.

[0015] 1. Materials and methods

[0016] 1.1 Materials

[0017] 1.1.1 Strain The starting strain: 08-28 (Streptomyces variegata S.bambergiensis) was provided by Inner Mongolia Zhongmu Bio-Pharmaceutical Co., Ltd.

[0018] 1.1.2 Reagents Flavomycin standard (1mg is equivalent to 1395 flavomycin units), China Veterinary Drug Administration; Streptomycin Sulfate (650μg / mg), imported; Ager, Japan; Dextrin, K 2 HPO 4 3H 2 O, Tianjin Fengchuan Chemical Reagent Technology Co., Ltd.; FeSO 4 ·7H 2 O, glucose, MgSO 4 ·7H 2 O, Tianjin Sitong Chemical Factory; Ammonium Formate, Peptone, Tianjin Guangfu Fine Chemical Research Institute; Yeast Extract, Beijing Aoboxing Biotechnology Co., Ltd.; Ascorbic Acid, Tianjin No. 3 Chemical Reagent Factory; Beef Extract, Tianjin Yingbo Biochemical Reagent Co., Ltd.; Amylase, Beijing Shuangxuan Microbial Culture Medium Products Factory; Tri...

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Abstract

The method discloses a method for mutating flavomycin producing bacteria by using a high-voltage corona electric field. The method is characterized in that the mutation voltage is 21kV, the needlepoint distance is 8mm, and the mutation time is 4min. A high-voltage corona electric field device adopted in the method is characterized in that the needlepoint distance is 20mm, the needle plate distance is 25mm, and the needlepoint diameter d is less than 0.01mm. As the flavomycin producing bacteria 08-28 is very sensitive to the electric field and the fatality rate of strains is increased thereupon along with the increasing of mutation dosage, and when the mutation voltage is 21kV, the needlepoint distance is 8mm and the mutation time is 4min, the mutation dosage is the best. Mutated strains No.3 are obtained through the mutation, screening and secondary culture of the electric field; and compared with the original strains, the mutated strains No.3 have the advantages that the capability of for producing the flavomycin is increased by 1.92 times, thereby showing that high-voltage corona electric field has better mutation effect on the flavomycin producing bacteria.

Description

technical field [0001] The invention relates to a method and a device for mutagenizing flavomycin-producing bacteria by using a high-voltage corona electric field. Background technique [0002] Flavomycin, also known as Moeno-mycin, Flavophospolipl, and Bam-bermycin, was developed by the German Hearst Company in the early 1970s. A new type of antibiotic growth promoter. Flavomycin is mainly composed of five microbial active substances, of which moenomycin A is the main component. This antibiotic is formed by anaerobic fermentation of fungi, exists in mycelium, and can be separated by extraction. Physicochemical analysis of the purified antibiotic showed that it was a phosphorus-containing sugar ester, different from any existing antibiotic. It has the characteristics of small dosage, remarkable growth-promoting effect, stable performance, no antagonistic effect with other additives, no resistance to drug resistance, no pollution, no residue, and high safety. Flavomycin ha...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12M1/42
CPCC12M35/02
Inventor 那日
Owner 那日
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