39results about How to "Rapid Typing" patented technology

The present invention provides the Free Fingers Typing Technology by fingers tapping on a surface, a technology that allows the user to type, point / select (on the display of a computer or computer-based-device) or play music with bare fingers without keyboard, without display pointing / selecting device and even without computer. The invention provides at least: one methodology to execute the finger movements, one convention to code the finger movements, two techniques to recognize the finger movements, a family of apparatus to optically monitor and recognize finger movements as well as the specifications of a plurality of related computer programmes all used with the objective of interpreting finger-surface taps and converting them into computer characters, keyboard key strokes, functions of display-pointing / selecting device, music notes etc. . . . The invention provides means for free fingers typing, pointing / selecting and music playing suitable for all devices requiring a keyboard such as computers, personal digital assistants, cellular phones, gaming devices, musical instruments or other keyboard or display pointing / selecting based devices

The present invention relates to a method and a mobile computing device for reliable and fast text entry. The method relies on a virtual keyboard layout that has a multi-touch interaction surface spread out on both the front and rear sides of a handheld computing device. A user-adaptive updating algorithm allows the virtual keyboard layout to adapt to the user's handling of the device.

The invention discloses an acute B-lymphocyte leukemia initiated cell phenetic classification kit. The kit is used in cooperation with a sever-color flow cytometer. The structure of the kit is configured with the following fluorescently-labeled monoclonal antibody: CD58FITC, CD10PE, CD34PerCP, CD38APC, CD19APC-Cy7 and CD45PacificBlue. The kit disclosed by the invention can be used for fast and accurately detecting the immunophenotype of the leukemia initiated cell of a B-ALL patient in first visit; and the CD58 expression proportion on the leukemia initiated cell tested by the kit provided by the invention can be used as one of the prognostic indicators of the B-ALL patient, and the indicator has important guiding significance in the prognosis of the B-ALL patient and the decision of a clinic treatment scheme.

The present invention relates to a method and a mobile computing device for reliable and fast text entry. The method relies on a virtual keyboard layout that has a multi-touch interaction surface spread out on both the front and rear sides of a handheld computing device. A user-adaptive updating algorithm allows the virtual keyboard layout to adapt to the user's handling of the device.

The invention discloses a method for detecting the single nucleotide polymorphism (SNP) of an ox PRDM16 gene, which comprises the following steps: carrying out PCR amplification on the ox PRDM16 geneby adopting ox whole-genome DNA to be detected, which contains the PRDM16 gene, as a template and adopting a primer pair P as primers; digesting a PCR amplification product by restriction endonucleaseMspl and then carrying out polyacrylamide gel electrophoresis on an amplified fragment after enzyme digestion; and authenticating the 212237th site SNP of the ox PRDM16 gene according to a polyacrylamide gel electrophoresis result. Because the PRDM16 gene function relates to birth weight, daily gain and body weight and growth characteristics, the detecting method lays foundation for establishingthe relation of the SNP of the PRDM16 gene and the growth characteristics so as to be used for the marker assisted selection (MAS) of the growth characteristics of a Chinese ox and rapidly establishing an ox population with favorable genetic resources.

The invention discloses an SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and an application thereof. A mark primer is at least one selected from Na12E02, KBRH138G23 or BrSS15. The SSR molecular mark provided by the invention can be applied in rapid identification and detection of the self-incompatibility of brassica campestris ssp. chinensis Makino, increase breeding efficiency and accelerate a breeding process.

The invention discloses animal chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method. The multiplex TaqMan-MGB probe real-time fluorescent PCR method can perform quick detection on Ch. Abortus, Ch.pecorum and Ch.psittaci. On the basis of the conserved region of the three chlamydia target sequences MOMP, the detection method designs three pairs of primers and three TaqMan-MHB probes. The method can quickly, efficiently, specifically and sensitively detect the target sequences by a two-step amplification process under simple reaction conditions, is simple to operate, does not need any expensive instrument or reagent, and has the advantages of no technical requirements for operating personnel, low detection cost and short detection time. The method can avoid cross contamination which can possibly occur due to agarose electrophoresis, thereby enhancing the detection accuracy and reliability.

The invention relates to the technical field of cell biology detection, and particularly relates to an accurate detection kit for typing of a tumor immune cell subset. The invention provides markers for single-cell immune cell typing detection and an antibody combination thereof. The combination covers 42 markers and metal pre-labeled antibodies thereof for immune basic detection, lymphoid cell extension detection and myeloid cell extension detection. The antibody combination and a mass spectrum flow cytometry are used for typing detection of immune cells, various detection requirements can bemet, accurate detection of complex tumor immune cell heterogeneity and rapid and accurate typing of the immune cell subset are achieved, single-staining control and calculation compensation of all channels are not needed, samples are effectively saved, and the detection efficiency is improved.

The invention belongs to the field of biotechnology and particularly relates to a compound amplification system and a kit for MSI (microsatellite instability) gene mutation detection. The compound amplification system for MSI gene mutation detection is provided and amplifies mononucleotide microsatellite gene sites and dinucleotide microsatellite gene sites simultaneously; the mononucleotide microsatellite gene sites comprise NR-21, NR-22, NR-24, NR-27, BAT-25, BAT-26 and MONO-27; the dinucleotide microsatellite gene sites comprise one or more of D2S123, D17S250, D18S34 and D5S346. The mononucleotide microsatellite sites and the dinucleotide microsatellite sites are combined for detection and analysis of MSI, the detection accuracy and sensitivity can be effectively improved, and the system can be used for quickly detecting typing of tumor to guide treatment.

The invention belongs to the technical field of nucleic acid detection, and particularly relates to a kit for detecting thalassaemia gene mutations. The kit includes amplification primers shown as SEQID NO:1-SEQ ID NO:10 and extension primers shown as SEQ ID NO:11-SEQ ID NO:28. The kit realizes the genotyping of various types of mutation hotspots of thalassaemia genes in the same reaction system,has both flexibility and scalability, is simple in operation and high in throughput and low in cost, and is of great significance for the screening, prenatal diagnosis and the like on people with thalassaemia.

The invention relates to the field of genetics, in particular to a method and a device for detecting SNP marker sites based on low-depth sequencing. The method comprises the following steps: acquiring genome DNA of an individual to be detected; performing individual low-depth whole genome sequencing on the genome DNA, and comparing a sequencing result to a reference genome to obtain polymorphic site information; based on a hidden Markov model, performing genetic typing on the polymorphic site information by utilizing a reference haplotype database, wherein the reference haplotype database comprises mutation site information of a breeding population to which the to-be-detected individual belongs. According to the invention, the low-depth sequencing data of a single sample is utilized to carry out high-accuracy and standardized genotyping of the SNP sites of tens of millions of orders of magnitude of the whole genome in an extremely short time.

The purpose is to provide a high-precision DNA typing method and kit that make full use of a high-throughput sequencer and eliminate the ambiguity derived from phase ambiguity. The invention provides an HLA DNA typing method characterized by including: (1) a step for providing a set of primers that each hybridize specifically to an upstream region and a downstream region of at least two genes selected from genes belonging to HLA class I and class II in the nucleotide sequence of the human genome and amplify under identical PCR conditions; (2) a step for amplifying these at least two genes in a test sample (DNA) simultaneously in the same container under identical PCR conditions using this set of primers; (3) a step for determining the nucleotide sequence of the PCR product; and (4) an optional step for conducting a homology search of a database.

The invention relates to a composition and kit for detecting polymorphism of a human MDR1 gene, a sample treatment method and an application thereof. The composition comprises primers for detecting polymorphism of the 1236 site of the human MDR1 gene and the primers are shown in the formulas of SEQ ID NO. 2, SEQ ID NO. 3 and SEQ ID NO. 4. Primers for detecting polymorphism of the 2677 site of thehuman MDR1 gene and are shown in the formulas of SEQ ID NO. 6, SEQ ID NO. 7 and SEQ ID NO. 8. Primers for detecting polymorphism of the 3435 site of the human MDR1 gene and are shown in the formulas of SEQ ID NO. 10, SEQ ID NO. 11 and SEQ ID NO. 12. The primers do not interfere with each other, have high specificity, and are excellent in sensitivity and repeatability.

The invention discloses KASP-based soybean core SNP markers and application thereof, and belongs to the technical field of molecular detection. The KASP-based SNP markers comprise 65 core SNP markers for identifying soybean resources. Compared with the conventional high-throughput SNP chip detection, the KASP-based soybean core SNP markers have the advantages that only a sequential method is needed for analysis during variety weight determination or purity analysis, and other sites do not need to be detected when different sites required by weight determination are reached; and therefore, the KASP markers developed by the invention have detection flexibility in the aspects of samples, the number of sites and the like, and are suitable for rapid typing of a large number of samples.

The invention discloses a detection method of a human platelet allogeneic antigen gene. The detection method comprises the following steps of: carrying out polymerase chain reaction on a primer marked by a first marker to obtain an amplification product; taking partial amplification product out and adding the partial amplification product into a buffer solution to obtain a mixture, wherein the buffer solution comprises a carrier marked by a second marker and a probe marked by a third marker; dropping the mixture to a reagent strip to obtain a detection result, wherein the reagent strip comprises a biological substance which can be specifically bound with the third marker. The detection method of the human platelet allogeneic antigen gene provided by the invention carries out HPA (Human Platelet Antigen) gene amplification by means of PCR (Polymerase Chain Reaction) method, achieves the fast typing of the HPA gene by using a lateral flow immune chromatography technology in combination, shortens detection time, improves detection sensitivity and is of great clinical significance and value in the fast typing of the HPA gene.

The invention discloses a method for quickly detecting CYP2C19 gene polymorphism based on a pyrosequencing technique. The method comprises the following steps: S1) extraction for genome DNA; S2) primer design for CYP2C19 gene polymorphic site; S3) PCR amplified reaction; S4) pyrosequencing; S5) genotype analysis; S6) Sanger sequencing verification. The primer of the CYP2C19 gene polymorphic site includes the gene sequences and pyrosequencing primers in 1kb scope upstream and downstream the CYP2C19*2(rs4244285), CYP2C19*3(rs4986893) and CYP2C19*17 (rs12248560) sites. The method for quickly detecting CYP2C19 gene polymorphism established by the invention can be used for quickly and accurately parting CYP2C19 genes and has the characteristics of high speed, accuracy and low cost.

The invention belongs to the fields of genomics and bioinformatics, relates to an HLA genotype-SNP linkage database, a construction method thereof, and a HLA typing method. Specifically, the construction method of the HLA genotype-SNP linkage database comprises the following steps: a) selecting one or more HLA loca sequences as a reference sequence; b) comparing the known type HLA genes in a conventional HLA database with the reference sequence to find out a difference site of the reference sequence i.e. a SNP site, and to obtain an SNP linkage relationship relative to the reference sequence for constructing the HLA genotype-SNP linkage database. The present invention also relates to a method for determining SNP linkage relationship of HLA gene, and an HLA typing device. The method of the present invention achieves low-cost, high-throughput, high-accuracy and high-resolution typing for HLA.

The invention provides a kit and a usage method thereof. The kit comprises one or more pairs of 16 upstream and downstream primer pairs for amplifying STR loci, namely, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, vWA, Penta E, Penta D and Amelogenin, wherein the sequences of the 16 pairs of upstream and downstream primers are shown as SEQ ID NO:1 to SEQ ID NO:32. By adopting the kit and the usage method provided by the invention, the related 16 pairs of upstream and downstream primers have the advantages of high specificity and high amplification efficiency, and genotyping of the 16 STR loci can be performed rapidly and accurately.

The invention provides a method for detecting a 146S antigen in a foot-and-mouth disease vaccine based on a capillary electrophoresis method and application thereof. The method comprises the followingsteps of introducing a water-phase sample of the foot-and-mouth disease vaccine into a capillary tube by adopting pressure sample introduction, carrying out electrophoretic separation, detecting andrecording a characteristic peak of a 146S antigen in the water-phase sample, then integrating to obtain a peak area of the characteristic peak, and then obtaining the concentration of the 146S antigenaccording to a quantitative standard curve. The method provided by the invention can be used for quantitatively detecting multiple serotype 146S antigens at the same time, can be used for detecting monovalent, bivalent or trivalent foot-and-mouth disease vaccines, has the advantages of low sample size, high sensitivity, high detection speed and the like, has important significance for realizing rapid sampling inspection of market vaccines and improving the market detection efficiency, and meanwhile, has huge application value in the aspects of product research and development and quality supervision of multivalent foot-and-mouth disease vaccines.

The invention provides a method for detecting a haplogroup of chondriogen, which relate to a method for detecting the characteristic site of an mtDNA (mitochondrial Deoxyribose Nucleic Acid) haplogroup by using an oligonucleotide probe set. In the method, the mutation of specific mtDNA is detected via judgment of an FAM (6-carboxy-fluorescein) fluorescence signal by taking FAM and HEX (5-hexachloro-fluorescein phosphoramidite) fluorescence signals as detection signals in an Mx3005PReal-timePCRAlleleDiscrimination-SNP's mode. The method has the advantages of reasonable design, capabilities of quickly and accurately classifying mtDNA haplogroups at low cost and detecting the characteristic site of any haplogroup, particular suitability for analysis of a largeer amount of samples, no limit from a specific site, application to determination of any individual mtDNA haplogroup, particularly low requirement on a nucleic acid template of a detection sample, and wide application range in the fields of medical jurisprudence, archaeology and the like.

The invention belongs to the technical field of nucleic acid detection, and particularly relates to a kit for detecting thalassaemia gene mutations. The kit includes amplification primers shown as SEQID NO:1-SEQ ID NO:10 and extension primers shown as SEQ ID NO:11-SEQ ID NO:28. The kit realizes the genotyping of various types of mutation hotspots of thalassaemia genes in the same reaction system,has both flexibility and scalability, is simple in operation and high in throughput and low in cost, and is of great significance for the screening, prenatal diagnosis and the like on people with thalassaemia.

The invention discloses a method for detecting the single nucleotide polymorphism of the cattle PRDM16 gene. The whole genome DNA of the cattle to be tested including the PRDM16 gene is used as a template, and the primer pair P is used as a primer to amplify the cattle PRDM16 gene by PCR; After digesting the PCR amplification product with the endonuclease MspI, polyacrylamide gel electrophoresis was performed on the amplified fragment after digestion; the single nucleotide polynucleotide at position 212237 of the cattle PRDM16 gene was identified according to the results of polyacrylamide gel electrophoresis. Morphology; since the PRDM16 gene function involves birth weight, daily weight gain, and body weight growth traits, the detection method provided by the present invention has laid a foundation for the establishment of the relationship between the SNP of the PRDM16 gene and the growth traits, so as to be used for the marker assistance of the growth traits of Chinese yellow cattle Selection (MAS) quickly establishes cattle populations with excellent genetic resources.

The purpose of the invention is to provide a high-precision DNA typing method and kit that eliminates the ambiguity caused by phase ambiguity by using a high-throughput sequencer. The present invention provides a DNA typing method for HLA, which is characterized in that it comprises: (1) preparing at least two genes that are specifically related to at least two genes selected from HLA class I and class II genes in the base sequence of the human genome. The step of hybridizing the upstream region and the downstream region of the gene and amplifying the primer set under the same PCR conditions; (2) using the aforementioned primer set to simultaneously amplify the test sample in the same container under the same PCR conditions ( (3) the step of determining the base sequence of the PCR product; and (4) optionally, the step of performing a homology search with a database.

The invention discloses double RT-PCR (reverse transcription-polymerase chain reaction) primer, kit and method for amplifying North America and European porcine blue ear disease viruses; the method has the advantages of high specificity, high sensitivity, low time and labor consumption and the like, and is of important guidance significance to molecular epidemiology, genetic evolution, vaccine research and development and the like for studying porcine blue ear disease. In addition, denaturing and annealing temperature in the method only takes 20 s, amplification can be performed as well just with one PCR apparatus, and the method may also act as a method for identifying and detecting North America and European porcine blue ear disease viruses and helps quickly classify porcine blue ear viruses.

The invention provides a kit and a using method thereof. The kit comprises one or more pairs of 16 pairs of upper and lower primer pairs for amplifying STR sites D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, vWA, Penta E, Penta D and Amelogenin, and the sequences of the 16 pairs of upstream and downstream primer pairs are shown in SEQ ID NO: 1-32. The16 pairs of upstream and downstream primer pairs related to the above-mentioned kit and the using method have the advantages of high specificity and high amplification efficiency, and can quickly andaccurately classify 16 STR sites.

The invention provides a visual membrane receptor chip, a preparation method and the applications thereof. The preparation method of the chip comprises the following steps of: making a membrane Si3N4 with a specific thickness on the surface of a silicon substrate so that a silver background presents on the surface of the silicon substrate; further coating a T-shaped polyamino alkaline polydimethylsiloxane membrane in a spinning way; and activating. A specific nucleotide sequence and SNP (Single Nucleotide Polymorphism) points (or point mutation) are detected by utilizing the visual membrane receptor chip with a silver background. When a specific target sequence is detected, the chip surface has visually recognizable color change from silver to blue or red. The detection technology is rapid, accurate and extremely economical and has low cost, high sensitivity and strong specificity and free selection between low flux and high flux. Compared with the visual membrane receptor chip with a gold background or the like, the visual membrane receptor chip provided by the invention ensures that the silver background provides stronger color comparison and has a clearer result in case of a blue signal, and the like.

The invention provides a group of probes and a kit containing the group of probes. The group of probes and the kit containing the group of probes are used for manufacturing a library building kit for detecting polymorphism of a pharmacogenomics related gene CYP3A4 by utilizing a hybrid capture method. According to the present invention, the genotype and the drug metabolism capability of CYP3A4 can be rapidly obtained through one-time detection, and the kit has advantages of simple operation, low time consumption and easy automatic detection, is especially suitable for the large sample size related research, and provides great significance for the discovery of the new genotype.

The invention discloses an acute B-lymphocyte leukemia initiated cell phenetic classification kit. The kit is used in cooperation with a sever-color flow cytometer. The structure of the kit is configured with the following fluorescently-labeled monoclonal antibody: CD58FITC, CD10PE, CD34PerCP, CD38APC, CD19APC-Cy7 and CD45PacificBlue. The kit disclosed by the invention can be used for fast and accurately detecting the immunophenotype of the leukemia initiated cell of a B-ALL patient in first visit; and the CD58 expression proportion on the leukemia initiated cell tested by the kit provided by the invention can be used as one of the prognostic indicators of the B-ALL patient, and the indicator has important guiding significance in the prognosis of the B-ALL patient and the decision of a clinic treatment scheme.

The invention discloses a lyssa virus (Lv) diagnosing and typing chip and a manufacturing method thereof, wherein the chip is a gene chip (or an oligonucleotide microarray) for Lv diagnosis and typing, and the method can simultaneously detect and type seven genotypes of Lv in a clinical sample and has the characteristics of rapidness, sensitivity, strong specificity, high accuracy and the like.