SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and application thereof
A head cabbage and molecular marker technology, applied in the fields of crop genetics, breeding and biology, can solve the problems of large workload, long detection period, easy to be restricted by environmental conditions, etc., and achieve the effect of improving reliability and rapid typing
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[0045] Example 1: Detection of self-incompatibility of non-heading Chinese cabbage parents and F2 generation individual plants
[0046] The specific implementation steps of this example are:
[0047] 1. Establishment of parental materials and hybrid populations:
[0048] The parents are two near-isogenic lines of non-heading Chinese cabbage variety 'Aijiaohuang': self-compatible line 002 and self-incompatible line 210, bred and preserved by the cabbage research group of Yu Horticultural College of Nanjing Agricultural University (Cao Shouchun , Hou Xilin, Hao Xiuming. Research on Breeding and Seed Propagation Techniques of Non-heading Chinese Cabbage Bantamia incompatibility Line. Journal of Nanjing Agricultural University, 2002, 25: 111-113). Among them, 002 showed strong self-incompatibility, and the affinity index was less than 1.0; 210 showed self-compatibility, and the affinity index was more than 5.0. Using 210 as the female parent and 002 as the male parent for cross-...
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