255results about How to "Wide range of clinical applications" patented technology

A method and apparatus for controlling blood withdrawal and infusion flow rate with the use of a pressure controller. The pressure controller uses pressure targets based upon occlusion limits that are calculated as a function of flow. The controller has the ability to switch from controlling withdrawal pressure to controlling infusion pressure based upon the detection of an occlusion. The controller distinguishes between partial and total occlusions of the withdrawal vein providing blood access. Depending on the nature of occlusion, the controller limits or temporarily reverses blood flow and, thus, prevents withdrawal vessel collapse or reverses blood flow to quickly infuse blood into the vessel without participation from operator.

A method and apparatus for controlling blood withdrawal and infusion flow rate with the use of a pressure controller. The pressure controller uses pressure targets based upon occlusion limits that are calculated as a function of flow. The controller has the ability to switch from controlling withdrawal pressure to controlling infusion pressure based upon the detection of an occlusion. The controller distinguishes between partial and total occlusions of the withdrawal vein providing blood access. Depending on the nature of occlusion, the controller limits or temporarily reverses blood flow and, thus, prevents withdrawal vessel collapse or reverses blood flow to quickly infuse blood into the vessel without participation from operator.

A method and apparatus for ultrafiltration of blood operating by removing blood from a peripheral blood vessel at a rate of less than two percent of total cardiac output of a patient, extracting fluid at a rate of 0.1 liter to 1.0 liters per hour while retaining cells and proteins in the blood, and returning the concentrated blood to a secondary blood vessel is disclosed. Blood is removed and returned using small gage needles. As a pump circulates blood from the patient, a filter removes ultrafiltrate from the blood using duty cycle or pump control, and a transparent container collects the removed fluid.

The invention discloses a kit for detecting high density lipoprotein cholesterol. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution I, 0.1-10 mmol / L polyanionic compound, 0.1-100 mmol / L bivalent cation, 0.1-10 mmol / L developer, 1-100 g / L surfactant I, 1-100 g / L surfactant II and 1-50 g / L preservative agent; and the reagent R2 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution II, 0.1-10 mmol / L 4-aminoantipyrine, 1-100 g / L surfactant III, 0.1-10 KU / L cholesterase, 0.1-10 KU / L cholesterol oxidase, 0.1-10 KU / L peroxidase and 1-50 g / L preservative agent. According to the invention, improvement is made on the basis of a selective inhibition method; the preferable compound surfactant pairs are added to inhibit and eliminate lipemia interference in the first reaction step, so that lipemia samples can be directly detected by a full-automatic biochemical analysis instrument without being pretreated; and the kit is simple to operate, improves the detection efficiency and lowers the detection cost.

The invention discloses a general-purpose nanochip for mass spectrometry, and relates to the technical field of mass spectrometry. The host material of the nanochip is silicon-based semiconductor material, array spotted holes are distributed at the surface of the host material, the inner surfaces of the spotted holes are nanostructures; the surface of the host material has regional hydrophobic modification, the internal portions of the array spotted holes are hydrophilic zones, and the outer portions of the array spotted holes are hydrophobic zones; or the internal portions of the array spotted holes are hydrophobic zones, and the outer portions of the array spotted holes are hydrophilic zones. The present invention further discloses a preparation method and a clinical application of the nanochip. The nanostructures can be used to extract the molecules at the surface of the sample of the biological tissue sample to be tested to improve the laser energy absorption and utilization so asto improve the ionization efficiency and enhance the mass spectrometry signals. The general-purpose nanochip can be widely applied to the field of clinical testing.

The invention relates to a monoclonal antibody and an antibody composition for neutralizing canine distemper virus (CDV), belonging to the technical field of biology. The monoclonal antibody 2G3 disclosed by the invention has high activity for neutralizing CDV and is used for identifying epitopes differently acting on CDV with a monoclonal antibody for neutralizing CDV prepared from another screened hybridoma cell 1D7 strain. Two monoclonal antibodies are prepared into the antibody composition, the neutralizing capability on CDV of which is obviously prior to the single monoclonal antibodies 2G3 and 1D7 in the composition; after being combined, the two monoclonal antibodies can generate antiviral synergistic enhancement action; and both the range and the capability for neutralizing CDV are increased. The monoclonal antibody disclosed by the invention is used for preparing the antibody composition for treating clinical canine distemper paroxysm animal; the virus neutralizing capability is stronger; failure of the monoclonal antibody caused by CDV heteromorphosis can be avoided; and the clinical application range is wider.

The invention discloses a primer, a method and a kit for detecting mutation sites of human BRCA1 and BRCA2 gene all-coding sequences. The primer comprises forward and reverse primers which can amplifyfor covering all the 24 coding zones of BRCA1 gene and of which base sequences are shown as SEQ ID NO:3-52. The primer further comprises forward and reverse primers which can amplify for covering allthe 27 exon coding zones of BRCA2 gene and of which base sequences are shown as SEQ ID NO: 53-134. A detection method based on NGS technique comprises the following steps: combining a specific primerwith a target template DNA sequence; adopting a universal primer for amplifying a to-be-detected sample target area; purifying a library by using a magnetic bead; performing high-throughput sequencing on the acquired library and analyzing mutation of BRCA1 and BRCA2 genes. The primer, method and kit disclosed by the invention can cover all the to-be-detected mutation sites; uniformity is high; experimental procedures can be simplified; detection sensitivity is promoted; a guidance is supplied for selective medication of patients suffering from familial breast cancer and ovarian cancer as wellas for genetic predisposition; morbidity risk is effectively reduced.

The invention belongs to the technical field of medicine, and provides an injectable PLGA porous composite microsphere preparation embedded with BMP-2 containing particles. A drug-carrying particle is formed by wrapping BMP-2 with hydrophilic materials such as chitosan, collagen or albumin; a microsphere with a 3D porous structure is constructed by the drug-carrying particle and a porous microsphere material PLGA; the drug-carrying particle is embedded inside the porous microsphere. The invention also provides a preparation method of the porous composite microsphere preparation, and an application in preparation of a repair material for defects of bone or cartilage tissue. The porous composite microsphere preparation of the invention can not only realize slow release of drugs at an administration site, but also facilitate proliferation and differentiation of porous supports of seed cells.

The medicated pillow includes pillow core and pillow cover, the pillow core has four medicine cavities fixed onto the upper surface filled with chrysanthemum, mulberry leaf, honeysuckle and gingko leaf separately; and the pillow cover has several independent medicine bags filled with Chinese medicine. The medicine cavities and bags are separated from the pillow core and pillow cover easily. The pillow is comfortable. Under the action of body's temperature, the effective components of the medicines in the medicines cavities and bags reach the meridians and acupoints of head to promote metabolism.

The invention discloses a detection kit for fusion mutation of ROS1 and various genes. The detection kit comprises a specific reverse transcription primer, a Q-PCR specific primer and a probe, wherein the nucleotide sequences of the Q-PCR specific prime and the probe comprise Mix 1: CD47; SDC4; SLC34A2 and ROS1 gene fusion, Mix 2: CD47; EZR; SDC4; SLC34A2 and ROS gene fusion, Mix 3: LRIG3; TPM3; GOPC and ROS1 gene fusion, Mix 4: GOPC and ROS1 gene fusion. By adopting a specific primer and probe technology, the detection kit disclosed by the invention can be used for specifically detecting human ROS1 gene fusion mutations. A real-time fluorescent PCR system is established for simultaneously detecting 15 fusion mutations of an RET gene; the detection kit is high in sensitivity, and 5-10-copy mutations can be detected; the detection kit is wide in sample detection range and high in detection speed.

The invention discloses a non-contact positioning auxiliary device used for the minimally invasive intracranial hematoma cleaning operation. The non-contact positioning auxiliary device comprises a fixed rack, a right-angle connecting rod, a right-angle connecting rod fixing shaft, a height adjusting device, a horizontal telescopic boom, a vertical telescopic boom, a vertical-direction laser alignment device and a horizontal-direction laser alignment device, wherein the far end of the horizontal telescopic boom is provided with the vertical-direction laser alignment device, the vertical-direction sector-shaped laser face emitted by the vertical-direction laser alignment device is vertical to the long shaft of the horizontal telescopic boom, a light source points to the downward direction, and a guiding datum line mark is provided; the far end of the vertical telescopic boom is provided with the horizontal-direction laser alignment device, the sector-shaped laser face emitted by the horizontal-direction laser alignment device can rotate along the optical axis of the laser through a rotation adjusting mechanism, and the sector-shaped laser face emitted by the horizontal-direction laser alignment device is enabled to be always vertical to the vertical-direction sector-shaped laser face through the right-angle-shaped adjusting booms. According to the non-contact positioning auxiliary device, the non-frame cantilever laser alignment guide technology is adopted, therefore, the puncture needle is accurately guided continuously during the operation; the non-contact design increases the visual operative field, and thus the steric hindrance in the puncture process is avoided.

The invention discloses a kit for detecting colorectal cancer susceptibility gene mutation and a detection method thereof. The kit contains specific primers, universal primers, PCR (Polymerase Chain Reaction) mixing reagents, positive controls and magnetic beads aiming at PIK3CA, KRAS and BRAF gene mutation sites. The method comprises the following steps: extracting genome DNA of a sample to be detected, combining the specific primers with target template DNA sequences, amplifying a target area of the sample to be detected by the universal primers, purifying a library by the magnetic beads, performing high-throughput sequencing on the obtained library, and analyzing mutation conditions. The method and the kit disclosed by the invention can simultaneously detect a plurality of susceptibility genes, simplify experimental procedures and simultaneously improve the detection sensitivity, are suitable for performing early screening and postoperative diagnosis of the colorectal cancer, and provide guide for individualized medication.