256results about How to "Wide range of clinical applications" patented technology

A method and apparatus for controlling blood withdrawal and infusion flow rate with the use of a pressure controller. The pressure controller uses pressure targets based upon occlusion limits that are calculated as a function of flow. The controller has the ability to switch from controlling withdrawal pressure to controlling infusion pressure based upon the detection of an occlusion. The controller distinguishes between partial and total occlusions of the withdrawal vein providing blood access. Depending on the nature of occlusion, the controller limits or temporarily reverses blood flow and, thus, prevents withdrawal vessel collapse or reverses blood flow to quickly infuse blood into the vessel without participation from operator.

A method and apparatus for ultrafiltration of blood operating by removing blood from a peripheral blood vessel at a rate of less than two percent of total cardiac output of a patient, extracting fluid at a rate of 0.1 liter to 1.0 liters per hour while retaining cells and proteins in the blood, and returning the concentrated blood to a secondary blood vessel is disclosed. Blood is removed and returned using small gage needles. As a pump circulates blood from the patient, a filter removes ultrafiltrate from the blood using duty cycle or pump control, and a transparent container collects the removed fluid.

A method and apparatus for controlling blood withdrawal and infusion flow rate with the use of a pressure controller. The pressure controller uses pressure targets based upon occlusion limits that are calculated as a function of flow. The controller has the ability to switch from controlling withdrawal pressure to controlling infusion pressure based upon the detection of an occlusion. The controller distinguishes between partial and total occlusions of the withdrawal vein providing blood access. Depending on the nature of occlusion, the controller limits or temporarily reverses blood flow and, thus, prevents withdrawal vessel collapse or reverses blood flow to quickly infuse blood into the vessel without participation from operator.

This invention provides a method for separating and extracting high-purity stachyose product from Rehmannia glutinosa and Stachys sieboldii. This invention also provides a method for continuously separating and extracting stachyose and catalpol from Rehmannia glutinosa. The method comprises: separating and purifying the extract by passing through continuous pressure-filtration, microfiltration, ultrafine filtration and nanofiltration membranes, and active carbon column, macroporous adsorption resin column, cation exchange resin column, and anion exchange resin column chromatography to obtain stachyose and catalpol. The method has scuh advantages as increased product quality, low wnwegy consumption, and increased raw material utility.

The invention discloses a DOA (depth of anesthesia) monitoring device which comprises an EEG (electroencephalogram) collecting electrode, a pre-arrangement module, a Fourier transform module, an Ac (activity of cortex) calculation module, an As (activity of subcortex) calculation module, a balanced DOA calculation module and a display module, wherein the pre-arrangement module comprises a pre-amplification module and an analog-digital conversion module which are connected sequentially; the Ac calculation module receives output data of the Fourier transform module and calculates subparameters of Ac; the As calculation module receives output data of the Fourier transform module and calculates subparameters of As; the balanced DOA calculation module receives subparameters output by the Ac calculation module and the As calculation module and obtains a balanced DOA with a weighted sum method; and the display module displays the balanced DOA. On the premise that the DOA monitoring accuracy in the state of pure inhalation or intravenous general anesthetics is reserved, the DOA monitoring device has the advantage that the DOA monitoring is more accurate in the state of opioid medicine composite inhalation or intravenous general anesthetics.

The invention belongs to the technical field of medicines. The invention provides an injectable medicated particle-inlaid porous composite microsphere preparation. The injectable medicated particle-inlaid porous composite microsphere preparation is prepared by the following steps: wrapping polypeptide and protein medicines or chemical medicines by using hydrophilic materials such as chitosan, collagen or albumin to form medicine-loading particles; constructing microspheres with three-dimensional (3D) porous structures by using medicated particles and porous microsphere materials; and inlaying the medicated particles in the porous microspheres. The invention also provides a preparation method of the porous composite microsphere preparation and an application of the porous composite microsphere preparation in preparation of tissue defect repair materials. The porous composite microsphere preparation provided by the invention can realize slow release of the medicines and is favorable for proliferation and differentiation of seed cells.

The invention discloses a kit for detecting high density lipoprotein cholesterol. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution I, 0.1-10 mmol / L polyanionic compound, 0.1-100 mmol / L bivalent cation, 0.1-10 mmol / L developer, 1-100 g / L surfactant I, 1-100 g / L surfactant II and 1-50 g / L preservative agent; and the reagent R2 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution II, 0.1-10 mmol / L 4-aminoantipyrine, 1-100 g / L surfactant III, 0.1-10 KU / L cholesterase, 0.1-10 KU / L cholesterol oxidase, 0.1-10 KU / L peroxidase and 1-50 g / L preservative agent. According to the invention, improvement is made on the basis of a selective inhibition method; the preferable compound surfactant pairs are added to inhibit and eliminate lipemia interference in the first reaction step, so that lipemia samples can be directly detected by a full-automatic biochemical analysis instrument without being pretreated; and the kit is simple to operate, improves the detection efficiency and lowers the detection cost.

The invention discloses a general-purpose nanochip for mass spectrometry, and relates to the technical field of mass spectrometry. The host material of the nanochip is silicon-based semiconductor material, array spotted holes are distributed at the surface of the host material, the inner surfaces of the spotted holes are nanostructures; the surface of the host material has regional hydrophobic modification, the internal portions of the array spotted holes are hydrophilic zones, and the outer portions of the array spotted holes are hydrophobic zones; or the internal portions of the array spotted holes are hydrophobic zones, and the outer portions of the array spotted holes are hydrophilic zones. The present invention further discloses a preparation method and a clinical application of the nanochip. The nanostructures can be used to extract the molecules at the surface of the sample of the biological tissue sample to be tested to improve the laser energy absorption and utilization so asto improve the ionization efficiency and enhance the mass spectrometry signals. The general-purpose nanochip can be widely applied to the field of clinical testing.

The invention relates to a monoclonal antibody and an antibody composition for neutralizing canine distemper virus (CDV), belonging to the technical field of biology. The monoclonal antibody 2G3 disclosed by the invention has high activity for neutralizing CDV and is used for identifying epitopes differently acting on CDV with a monoclonal antibody for neutralizing CDV prepared from another screened hybridoma cell 1D7 strain. Two monoclonal antibodies are prepared into the antibody composition, the neutralizing capability on CDV of which is obviously prior to the single monoclonal antibodies 2G3 and 1D7 in the composition; after being combined, the two monoclonal antibodies can generate antiviral synergistic enhancement action; and both the range and the capability for neutralizing CDV are increased. The monoclonal antibody disclosed by the invention is used for preparing the antibody composition for treating clinical canine distemper paroxysm animal; the virus neutralizing capability is stronger; failure of the monoclonal antibody caused by CDV heteromorphosis can be avoided; and the clinical application range is wider.

The invention discloses a primer, a probe and a detection kit for detection of an EML4-ALK fusion gene mutation. The primer and probe provided by the invention are SEQ ID NO 1-SEQ ID NO 24. The primer and probe of the invention can carry out specific detection on EML4-ALK fusion gene mutation. The present invention has the following advantages: (1) an established real-time fluorescent PCR system capable of simultaneously detecting 9 kinds of fusion mutation of EML4-ALK gene; (2) high sensitivity capable of detecting mutation of 5-10 copies; (3) simple operation, cheap detection and wide clinical application range; (4) a wide range of samples from fresh pathological tissue to paraffin embedded tissue or pleural fluid; and (5) high speed detection with a detection procedure taking only 90 minutes.

The invention provides a sinomenine injection special for intravenous administration. The sinomenine injection is an injection which comprises 0.005-0.3wt% of sinomenine and an aqueous solvent for injection, or an injection which comprises sterile injection powder or lyophilized injection powder used for preparation just before injection to cause the sinomenine concentration to be 0.005-0.3wt% in the injection and the aqueous solvent for injection. The sinomenine injection of the invention is used for treating rheumatism, chronic pain and other chronic inflammatory diseases. Compared with other existing injection forms, the sinomenine injection special for intravenous injection has lower drug adverse reaction, and the clinical curative effect is obviously improved.

The invention discloses a primer, a method and a kit for detecting mutation sites of human BRCA1 and BRCA2 gene all-coding sequences. The primer comprises forward and reverse primers which can amplifyfor covering all the 24 coding zones of BRCA1 gene and of which base sequences are shown as SEQ ID NO:3-52. The primer further comprises forward and reverse primers which can amplify for covering allthe 27 exon coding zones of BRCA2 gene and of which base sequences are shown as SEQ ID NO: 53-134. A detection method based on NGS technique comprises the following steps: combining a specific primerwith a target template DNA sequence; adopting a universal primer for amplifying a to-be-detected sample target area; purifying a library by using a magnetic bead; performing high-throughput sequencing on the acquired library and analyzing mutation of BRCA1 and BRCA2 genes. The primer, method and kit disclosed by the invention can cover all the to-be-detected mutation sites; uniformity is high; experimental procedures can be simplified; detection sensitivity is promoted; a guidance is supplied for selective medication of patients suffering from familial breast cancer and ovarian cancer as wellas for genetic predisposition; morbidity risk is effectively reduced.

The invention relates to an electronic biosensor based on single electric charge detection; the invention discloses an electronic sensor of an ion-gated bipolar amplifier (IGBA); the electronic sensor comprises a combined type MOSFET, a BJT and a sample container connected with a floating gate electrode layer of the MOSFET; the electronic sensor has very high sensitivity, and can be used in solution ion detection, biology molecular detection and gas detection.

The invention discloses a polycaprolactone / calcium sulfate composite material and a preparation method thereof, belonging to the field of macromolecule modification. The method comprises the following steps: utilizing biosurfactant to carry out surface modification on anhydrous calcium sulfate or calcium sulfate whiskers; and adopting a coprecipitation and mould pressing method to prepare anhydrous calcium sulfate or calcium sulfate whiskers reinforced polycaprolactone composite material. The composite material prepared by the method in the invention has the advantages of high mechanical strength and adjustable performance, thereby laying a foundation for the application thereof.

The invention belongs to the technical field of medicine, and provides an injectable PLGA porous composite microsphere preparation embedded with BMP-2 containing particles. A drug-carrying particle is formed by wrapping BMP-2 with hydrophilic materials such as chitosan, collagen or albumin; a microsphere with a 3D porous structure is constructed by the drug-carrying particle and a porous microsphere material PLGA; the drug-carrying particle is embedded inside the porous microsphere. The invention also provides a preparation method of the porous composite microsphere preparation, and an application in preparation of a repair material for defects of bone or cartilage tissue. The porous composite microsphere preparation of the invention can not only realize slow release of drugs at an administration site, but also facilitate proliferation and differentiation of porous supports of seed cells.

The invention provides an automatic measuring method and device for the volume change of a skeletal muscle. The device includes a data acquisition module, an image preprocessing module, a measurement module and a quantitative evaluation module, wherein the data acquisition module is combined with ultrasonic imaging equipment and collects a skeletal muscle cross-sectional ultrasound image, the image preprocessing module is used for reducing big speckle noise in the ultrasound image and enhancing the interest region contour of the image, the measurement module is used for extracting the interest region contour of the preprocessed ultrasound image, and the quantitative evaluation module is used for the quantitative analysis of the automatically extracted regional contour. The method and the device can automatically measure the volume change of the skeletal muscle, and have important clinical application value, for example, and a quantitative basis can be provided for the clinical diagnosis and rehabilitation evaluation of patients with sarcopenia.

The present invention belongs to the field of biomedical engineering, in the concrete, it relates to a photosensitive scaffold with the function of preventing and curing restenosis. The photosensitizer can be used to modify the scaffold so as to obtain photosensitive scaffold, said photosensitive scaffold is placed in the pathological lumen, and irradiated by light, so that the photosensitizer on the scaffold can be activated so as to produce photochemical reaction to form active oxygen substance, resist endometrial hyperplasia / and prevent production of scaffold restricture.

The invention discloses probes, primers, a detection system and a kit for detecting mutations of an EGFR gene, and the probes and the primers have the sequences of SEQ ID NO.1 to SEQ ID NO.24. The invention is characterized in that (1), the amplification efficiency is greatly improved to a largest extent; (2) the sensitivity is high, and the detection sensitivity can reach 0.2%; (3) compared with a digital PCR method, operations are simple, the cost is saved, and the clinical application scope is wide; (4) plasma samples with large reaction volume are detected, the DNA sampling quantity of the plasma samples becomes larger, the system is more stable, and the detection rate of the plasma samples is increased; (5) 25 mutations of the EGFR gene can be detected simultaneously in three reaction tubes, and results are intuitive and clear; (6) the detection speed is fast, and consumed time is only 1 / 2 that of the digital PCR; and (7) a detection method can detect peripheral blood samples, has convenient sampling, and can dynamically monitor curative effect of EGFR-TKI drugs on patients.

The medicated pillow includes pillow core and pillow cover, the pillow core has four medicine cavities fixed onto the upper surface filled with chrysanthemum, mulberry leaf, honeysuckle and gingko leaf separately; and the pillow cover has several independent medicine bags filled with Chinese medicine. The medicine cavities and bags are separated from the pillow core and pillow cover easily. The pillow is comfortable. Under the action of body's temperature, the effective components of the medicines in the medicines cavities and bags reach the meridians and acupoints of head to promote metabolism.

The invention discloses a compound acetaminophen injection for livestock and its preparation method. the injection comprises acetaminophen 50-200, diclofenac sodium 10-50, polyethylene glycol-400 100-500, propylene glycol 100-500, and sodium bisulfite 10-30. the preparation method comprises (1) mixing polyethylene glycol-400 and propylene glycol, heating to 70-80deg.C, adding diclofenac sodium, stirring, adding acetaminophen, stirring; (2) heating water for injection to 30-40deg.C, adding sodium bisulfite, stirring; and (3) merging the solutions of steps 1 and 2, adjusting pH to 4.5-6.5 with hydrochloric acid or sodium hydroxide, and adding water for injection to 1000 to obtain the final product. The invention strengthen antipyretic and analgesic effects, is effective for treating pain and fever due to different kinds of inflammation, and can improve animal survival rate and increase animal body weight.

The invention discloses a detection kit for fusion mutation of ROS1 and various genes. The detection kit comprises a specific reverse transcription primer, a Q-PCR specific primer and a probe, wherein the nucleotide sequences of the Q-PCR specific prime and the probe comprise Mix 1: CD47; SDC4; SLC34A2 and ROS1 gene fusion, Mix 2: CD47; EZR; SDC4; SLC34A2 and ROS gene fusion, Mix 3: LRIG3; TPM3; GOPC and ROS1 gene fusion, Mix 4: GOPC and ROS1 gene fusion. By adopting a specific primer and probe technology, the detection kit disclosed by the invention can be used for specifically detecting human ROS1 gene fusion mutations. A real-time fluorescent PCR system is established for simultaneously detecting 15 fusion mutations of an RET gene; the detection kit is high in sensitivity, and 5-10-copy mutations can be detected; the detection kit is wide in sample detection range and high in detection speed.

The invention discloses a non-contact positioning auxiliary device used for the minimally invasive intracranial hematoma cleaning operation. The non-contact positioning auxiliary device comprises a fixed rack, a right-angle connecting rod, a right-angle connecting rod fixing shaft, a height adjusting device, a horizontal telescopic boom, a vertical telescopic boom, a vertical-direction laser alignment device and a horizontal-direction laser alignment device, wherein the far end of the horizontal telescopic boom is provided with the vertical-direction laser alignment device, the vertical-direction sector-shaped laser face emitted by the vertical-direction laser alignment device is vertical to the long shaft of the horizontal telescopic boom, a light source points to the downward direction, and a guiding datum line mark is provided; the far end of the vertical telescopic boom is provided with the horizontal-direction laser alignment device, the sector-shaped laser face emitted by the horizontal-direction laser alignment device can rotate along the optical axis of the laser through a rotation adjusting mechanism, and the sector-shaped laser face emitted by the horizontal-direction laser alignment device is enabled to be always vertical to the vertical-direction sector-shaped laser face through the right-angle-shaped adjusting booms. According to the non-contact positioning auxiliary device, the non-frame cantilever laser alignment guide technology is adopted, therefore, the puncture needle is accurately guided continuously during the operation; the non-contact design increases the visual operative field, and thus the steric hindrance in the puncture process is avoided.

An injection-type composite bone repair material for treating bone fracture, nonunion, dysostosis and osteoporosis contains the fibrin in mammal's blood as carrier, genetically recombinant bone morphogenetic protein, fibroblast growth factor and beta-converting growth factor. Its advantages are sure curative effect, wide application range and high safety.

The invention discloses a kit for detecting colorectal cancer susceptibility gene mutation and a detection method thereof. The kit contains specific primers, universal primers, PCR (Polymerase Chain Reaction) mixing reagents, positive controls and magnetic beads aiming at PIK3CA, KRAS and BRAF gene mutation sites. The method comprises the following steps: extracting genome DNA of a sample to be detected, combining the specific primers with target template DNA sequences, amplifying a target area of the sample to be detected by the universal primers, purifying a library by the magnetic beads, performing high-throughput sequencing on the obtained library, and analyzing mutation conditions. The method and the kit disclosed by the invention can simultaneously detect a plurality of susceptibility genes, simplify experimental procedures and simultaneously improve the detection sensitivity, are suitable for performing early screening and postoperative diagnosis of the colorectal cancer, and provide guide for individualized medication.

The invention relates to application of sinomenine as a medicament for preventing and treating pulmonary interstitial fibrosis. The medicament is prepared by sinomenine or pharmaceutically acceptable salt thereof and other adjuvants, wherein sinomenine or pharmaceutically acceptable salt thereof serves as an activating agent. The routes of administration of the medicament include intravenous drip, intramuscular injection, oral administration, transdermal absorption, atomization inhalation and bronchoalveolar lavage. Experiments show that sinomenine does not have obvious difference with the dexamethasone control group in the effect on inhibiting formation of bleomycin-induced pulmonary fibrosis in mice and prove that sinomenine can alleviate pulmonary alveolitis and fibrosis degrees of themice with bleomycin-induced pulmonary fibrosis and the mechanisms are probably realized by inhibiting expressions of TGF (transforming growth factor)-beta1 and alpha-SMA (smooth muscle actin) and thecontent of HYP (hydroxyproline).

The invention discloses probes, primers and a kit for detecting a T790M mutation of an EGFR gene. The probes and the primers have the following sequence: SEQ ID NO: 01 to SEQ ID NO. 07. The probes, the primers and the kit have the following benefits: (1) SNP sites on the primers are designed as G / A merged basic groups, so that all efficiencies are compatible, and the amplification efficiency is improved; (2) the sensitivity is high, that is, the detection sensitivity can reach 2 permillage; (3) compared with those adopting a digital PCR method, the operation is simple, the cost is reduced, and the clinical application range is wide; (4) through blood plasma sample detection with a large reaction volume, the DNA loading quantity of blood plasma samples is increased, the detection system is more stable, and the detection rate of the blood plasma samples is improved; (5) the detection speed is high, that is, the detection process can be completed within only 120 minutes, and the time consumed in the detection process is only a half of that consumed according to the digital PCR method; (6) the probes, the primers and the kit, provided by the invention, can be utilized for detecting peripheral blood samples, so that convenient sampling and dynamic detection can be realized.

A liposome preparation of ribavirin used as an antivirus medicine is disclosed. Said liposome preparation is the artificial cells, which can carry the ribavirin into the cells infected by virus, resulting in high effect and low toxin. It is prepared through dissolving ribavirin in the buffering solution of phosphate, adding the mixture of phosphatide, cholesterin and VE, heating to 58-62 deg.C, and maintaining the temp for 30 min to obtain uniform emulsion.

The invention discloses a primer and a probe for detecting an RET fusion gene. The primer comprises an RET fusion mutation specificity reverse transcription primer, an RET gene fusion mutation detection specificity primer and a probe nucleotide sequence. The invention discloses a detection reagent kit for detecting the RET fusion gene. The detection reagent kit comprises an mRNA reverse transcription cDNA system, wherein the mRNA reverse transcription cDNA system is used for detecting the PCR reaction of the detection reagent. The specific primer and probe technology is adopted, so that the human RET gene fusion mutation can be specifically detected; a real-time fluorescent PCR system is established, and 17 fusion mutations of the RET genes can be simultaneously detected; the sensitivity is high, the mutation of 5 to 10 copies can be detected; the operation is simple, the detection is cheap, and the clinical application range is wide; the specimen detection range is wide, and the specimen can be fresh pathological tissues, paraffin-embedded tissues or pleural fluid; the detection speed is high, and the detection process can be completed in 90 minutes.

The invention belongs to the technical field of medicine, and provides a PLGA (poly(lactic-co-glycolic acid)) porous composite microsphere preparation embedded with BMP-2 and TGF-beta1 containing microspheres therein, which not only can bring about benefits for the adhesion and the growth of seed cells by virtue of a 3D porous structure but also can undergo controlled- and sustained-release and can be applied to injection. The invention also provides a preparation method of the porous composite microsphere preparation as well as application of the porous composite microsphere preparation in preparing bone or cartilage tissue defect repairing materials. The porous composite microsphere disclosed by the invention, which can serve as a good carrier for cell adhesion and proliferation and can be directly injected to a target part, can offer a possibility of non-surgical repair to bone and cartilage tissue injuries; and the porous composite microsphere preparation is extensive in clinical application and market prospect.