The invention discloses a preparation method of a medical antibacterial bandage. The preparation method comprises spinning, weaving of a blank towel, preparation of an antibacterial liquid, preparation of an antibacterial soaking liquid, modification treatment, softening and drying. The modification treatment process comprises dipping a blank towel obtained by the step b into a washing machine, carrying out cleaning through water at 95-100 DEG C according to a bath ratio of 1: 20-27 for 25-36min, cooling the blank towel to 5-10 DEG C, washing the blank towel through water for 4-8min, adding anantibacterial soaking solution into the water, adjusting the temperature of the water solution to 65-75 DEG C, and carrying out soaking through the washing machine for 40-50min. The drying process comprises taking out a bandage and carrying out freeze drying to obtain gel on the surface of the bandage. The invention provides the preparation method of the medical antibacterial bandage. The medicalantibacterial bandage has the traditional sterilization and antibacterial functions and a cleaning function, has effects of improving blood circulation, promoting metabolism, eliminating fatigue andrefreshing brain, feels smooth, supple and comfortable, absorbs moisture and is breathable and comfortable.