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Photosensitive PRP (platelet-rich plasma) gel and preparation method and application thereof

A platelet-rich plasma and gel technology used in the field of regenerative medicine to achieve seamless integration

Active Publication Date: 2017-06-13
ZHONGSHAN GUANGHE MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are related studies that load PRP into scaffold materials constructed of hyaluronic acid, gelatin, chitosan, collagen and other substances to form PRP composite gel, which realizes the controllable release of PRP internal factors to a certain extent, but these The composite gel is also pre-prepared in vitro, and it is difficult to seamlessly and firmly combine with the wound site

Method used

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  • Photosensitive PRP (platelet-rich plasma) gel and preparation method and application thereof
  • Photosensitive PRP (platelet-rich plasma) gel and preparation method and application thereof
  • Photosensitive PRP (platelet-rich plasma) gel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Preparation of photosensitive PRP gel formed from carboxymethylcellulose modified with photosensitive group o-nitrobenzyl alcohol (CMC-NB) and rabbit blood PRP, and its rheological and morphology analysis and tissue adhesion test

[0061] (1) Synthesis of photosensitive group o-nitrobenzyl alcohol (NB).

[0062]

[0063] Synthesis of Compound 1: References [Pauloehrl, T.; Delaittre, G.; Bruns, M.; Meiβler, M.; H.G.; Bastmeyer, M.; Barner-Kowollik, C.Angew.Chem.Int.Ed.2012,51,9181.]

[0064] Synthesis of compound 2: Dissolve compound 1 (1g, 3.3mmol) and ethylenediamine (1.1mL) in methanol (50mL), reflux for overnight reaction, rotary evaporation under reduced pressure, dissolve the crude product in methanol, and dissolve in acetic acid Reprecipitate from ethyl ester. After several times of dissolution-reprecipitation, filtration and vacuum drying, the pure compound 2 (0.93 g, yield 85%) was obtained.

[0065] (2) Synthesis of CMC-NB: 500mg of carboxymethylcellulos...

Embodiment 2

[0072] Photosensitive PRP hydrogel for slow release of cytokines

[0073] Dissolve CMC-NB in ​​physiological saline, configure a solution with a mass concentration of 4%, and sterilize at high temperature. Take a certain volume of freshly extracted rabbit blood PRP and mix it with the above CMC-NB solution to form a gel precursor solution. Subsequently, 200 μL of the gel precursor solution was added to the wells of the 48-well plate, and each well was irradiated with light (365nm LED light source, light intensity: 20mW / cm2) for 5 minutes to form a photosensitive PRP gel as the experimental group. In addition, in the control group, 160 μL of freshly extracted rabbit blood PRP was added dropwise to a 48-well plate, and then 40 μL of 10% wt calcium chloride solution containing 1000 U / mL thrombin was added, and mixed evenly until a PRP gel was formed. Next, 200 μL of PBS was added to the wells of the experimental group and the control group, and the last added PBS was collected e...

Embodiment 3

[0075] Effect of photosensitive PRP hydrogel on repairing skin damage in diabetic rats

[0076] Preparation of the skin defect model of type Ⅱ diabetic rats: take 20 normal SD rats, and use standard methods to induce diabetes in SD rats (references Mohsen Khosravi Maharlooei, Mansooreh Bagheri, ZhabizSolhjou, Behnam Moein Jahromi, Majid Akrami, Lili Rohani , Ahmad Monabati, Ali Noorafshan, Gholamhossein Ranjbar Omrani, Diabetes Research And Clinical Practice 2011, 93, 228-234). Subsequently, a complete skin defect model with a diameter of 2 cm was made on the back of each SD rat. And according to the different treatment methods of the wound site, the 20 SD rats were randomly divided into five groups on average: the wounds of the SD rats in the experimental group were treated with 200 μL of photosensitive PRP gel formed in situ; the SD rats in the positive control group The wounds were treated with 200 μL of freshly extracted rabbit blood PRP gel; the wounds of SD rats in the ...

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Abstract

The invention relates to a photosensitive PRP (platelet-rich plasma) gel and a preparation method and application thereof. The preparation method comprises the following steps of mixing a biocompatible medium solution of a macromolecule modified by an o-nitrobenzyl photo response group and an extracted PRP according to a certain ratio, so as to form a gel precursor solution; then, radiating the gel precursor solution by light, and enabling the o-nitrobenzyl group in the macromolecule modified by the o-nitrobenzyl photo response group to generate photochemical reaction under the excitation action of a light source, so as to produce an aldehyde functional group; generating coupling reaction with amino distributed at the surface of the protein in the PRP to form an imine bond, so as to realize the preparation of the photosensitive PRP gel. Compared with the prior art, the photosensitive PRP gel prepared by the method has the advantages that the internal cell factors can be slowly released, and the tight and seamless bonding between the PRP gel and wounds is realized.

Description

technical field [0001] The invention relates to the technical field of regenerative medicine, in particular to a photosensitive platelet-rich plasma gel and its preparation method and application. Background technique [0002] Platelet-rich plasma (platelet-rich plasma, PRP) is the plasma containing high concentration of platelets obtained by centrifuging animal or human whole blood. PRP contains a number of growth factors, including platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-beta), insulin-like growth factor (IGF), epithelial growth factor (EGF), and vascular epithelial growth factor (VEGF) etc., can effectively promote wound healing. In 1997, Whitman first proposed the concept of autologous platelet-rich gel (APG), which overcame the immune rejection faced by allogeneic plasma, and then autologous PRP was successively used in orthopedics, maxillofacial surgery, burns and plastic surgery, etc. Treatment of field wounds. At present, PRP ha...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K35/19A61K47/60A61K47/61A61K47/54A61K9/06A61K9/00A61L27/20A61L27/18A61L27/50A61L27/54A61L24/06A61L24/08A61L24/00
CPCA61K9/0009A61K9/06A61K35/16A61K35/19A61L24/001A61L24/0015A61L24/06A61L24/08A61L27/18A61L27/20A61L27/50A61L27/54A61L2300/414A61L2430/02A61L2430/06A61L2430/10A61L2430/12A61L2430/32A61L2430/38
Inventor 汪洪朱麟勇林秋宁汪泱
Owner ZHONGSHAN GUANGHE MEDICAL TECH CO LTD
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