GeXP multi-primers and method for detecting akabane viruses, bovine viral diarrhea viruses and foot and mouth disease viruses
A technology for bovine viral diarrhea and foot-and-mouth disease, applied in the field of biotechnology applications, can solve the problems of high price, long measurement period, and cumbersome operation
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specific Embodiment approach
[0026] Example 1 Primer Verification
[0027] The singleplex RT-PCR product was analyzed by capillary electrophoresis on GeXP system. The size of the amplified fragments of each target gene was AKAV: 267-270bp, BVDV: 349-354bp, FMDV: 370-375bp, and the size of the amplified fragments was consistent with the design.
Embodiment 2
[0028] Example 2 Establishment of Multiplex Detection System and Verification Results of Single Template Specificity
[0029]In the multi-primer detection system, only a specific fragment of a single virus template was amplified in each reaction without cross-reaction, suggesting that this method has strong specificity and can distinguish and distinguish each virus according to the size of the amplified fragment. The results are shown in Figure 1-Figure 4 .
Embodiment 3
[0030] Embodiment 3 multiple detection system single template sensitivity test result
[0031] Using cloned plasmids to transcribe RNA in vitro as a template, the detection limit of each virus was 100 copies / μL.
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