The invention belongs to the technical field of food processing, and particularly relates to a stuffing for an iron stick yam sesame ball with an iron stick yam as a main stuffing, an iron stick yam sesame ball and a preparation method thereof. The stuffing for the iron stick yam sesame ball comprises the following materials in parts by weight: 20-40 parts of iron stick yam granules, 20-30 parts of white granulated sugar, 20-30 parts of pureed carrots, 1-3 parts of salad oil and 3-5 parts of wheat starch. The iron stick yam sesame ball comprises the following materials in parts by weight: 19-22 parts of dough sheets, 1-3 parts of sesame and 2-5 parts of stuffings, wherein the dough sheet comprises the white granulated sugar, glutinous rice flour, a sesame ball improver and alkali powder. The iron stick yam sesame ball is prepared through material selection, cleaning, cutting, blending, stirring, forming the stuffings, dough kneading, making, quick-freezing, inspection, packaging and warehousing. The stuffing disclosed by the invention has medicinal and edible characteristics of the iron stick yam by adopting the iron stick yam as the main stuffing of the iron stick yam sesame ball, solves the problem that the phenomena of indigestion, dyspepsia and the like are easily caused on the traditional sesame ball, enables the sesame ball to be easily digested and absorbed and eliminates the fishy and greasy feeling of the traditional sesame ball on taste.