Primer and method for detecting mycoplasma, application of primer and product applying primer

Abstract

The invention provides a primer and a method for detecting a mycoplasma, application of the primer and a product applying the primer, and relate to the technical field of biology. The primer for detecting the mycoplasma can simultaneously amplify various mycoplasma 16SrDNA conserved regions. When the primer is applied to mycoplasma detection, various kinds of mycoplasmas can be simultaneously detected; broad spectrum performance is realized; the application range is enlarged. The method for detecting the mycoplasma has the advantages that the detection speed is high; the broad spectrum performance is high; the sensitivity is high; various samples can be simultaneously detected; the detection cost is reduced. The problem that a product and a method which have wide broad spectrum and can simultaneously and fast detect various mycoplasmas are lacked in the prior art is solved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer for detecting mycoplasma and its application, as well as a product and a method thereof. Background technique [0002] Mycoplasma (Mycoplasma), also known as Mycoplasma, is the smallest and simplest prokaryote found so far. The only visible organelle in Mycoplasma is ribosome. [0003] Mycoplasma contamination is one of the most common contaminations in the cell culture process, especially in the culture process of mammalian cells. The main reasons are: Mycoplasma is very small in size, with a diameter of 0.1 μm, which cannot be removed by general filter sterilization, and is difficult to remove under an optical microscope. See its morphological structure clearly; mycoplasma is tenacious and can survive at pH 7.6-8.0, and is resistant to penicillin; in the early stage of mycoplasma contamination, some sensitive cells can see that the growth and proliferation of the sensitive...

AI Technical Summary

Problems solved by technology

For relevant scientific researchers, the most serious consequence of mycoplasma contamination is that the contamination unknowingly changes many parameters and experimental results, such as changes in cytokine transcription and expression levels, the effect of viruses on cell viability and proliferation, foreign substances Regulatory effects on cell physiology, etc., reduce the credibility of research results or even get wrong data

And due to pollution, it is necessary to repurchase or resuscitate cells to regain cells that can grow stably and pass on, which causes a huge waste of time and research costs for scientific researchers.

[0006] Commonly used detection methods for mycoplasma include traditional culture method, ELISA method, PCR method, etc. At present, PCR method is the mainstream detection method for mycoplasma, but there are still many problems in the existing PCR detection method for mycoplasma, for example, insufficient sensitivity or specificity Wait

Moreover, the current mainstream kits can only target a certain type of mycoplasma, and their broad-spectrum is poor. They are not suitable for the actual situation that there are actually many kinds of mycoplasma that can contaminate cells, and are not suitable for actual scientific research and laboratory applications.

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