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Biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables

A fungal and biocontrol technology, applied in the direction of bacteria, biocides, microorganisms, etc., to achieve the effect of strong broad-spectrum and high genetic stability

Active Publication Date: 2017-06-13
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The problem solved by the present invention is to overcome the defects of the above-mentioned method of using chemical agents to prevent and treat apple tree leaf diseases, and to provide a method for preventing and treating fungal diseases of apple trees such as apple anthracnose leaf blight and apple leaf spot, as well as tomato early blight and watermelon wilt. Streptomyces cacaoi FN, a biocontrol agent for other fruit and vegetable fungal diseases, uses Streptomyces cacaoi FN to prepare Streptomyces biocontrol agents for the control of apple tree leaf diseases, which can not only completely replace chemical control methods, but also exclude food Potential safety hazards, and have achieved outstanding results in the effectiveness of preventing and controlling apple tree leaf diseases and broad-spectrum antibacterial properties

Method used

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  • Biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables
  • Biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables
  • Biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Isolation, purification and screening of Streptomyces cacaoi FN

[0063] 1. Isolation and purification of Streptomyces cacaoi FN

[0064] Collect soil samples from Funing Vegetable Garden and put them into sterile bags, take 10g and put them into a sterilized triangular flask containing 100mL distilled water and glass beads, place on a shaker at 200rpm for 30min, let stand for 10min, shake well and use a pipette Draw 5mL from the liquid device and add it to a small triangle bottle containing 45mL sterile water, that is, dilute 10 2 times, sequentially serially diluted to 10 -3 、10 -4 、10 -5 、10 -6 Use a pipette to draw 100 μl and spread it on a 2216E plate containing antibiotics (potassium dichromate 50 mg / L, nalidixic acid 15 mg / L and nystatin 50 mg / L), and set 3 replicates for each concentration , placed in a 28°C incubator for 5-10 days. Use a sterilized bamboo stick to pick a single colony of actinomycetes and put it on a freshly prepared 2216E plate...

Embodiment 2

[0072] The growth characteristic of embodiment 2 cocoa Streptomyces FN

[0073] 1. Growth temperature

[0074] Dispense 2216E liquid culture medium (bacteria peptone No.2 5g / L, yeast powder 1g / L, the balance is distilled water, natural pH) into transparent test tubes, and sterilize with damp heat at 121°C for 30 minutes. There should be no precipitation in the culture medium. Streptomyces cocoa FN was inoculated with shaker flask 48 in 2216E liquid medium as an inoculant, and was inoculated into test tubes containing 2216E liquid medium at a rate of 1‰, and the control was not inoculated. Cultivate at 0°C, 7°C, 15°C, 20°C, 28°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C incubators for 3d-5d, set 3 repetitions, observe And record the growth (precipitation) situation.

[0075] 2. Acid and alkali resistance

[0076] Adjust the 2216E liquid medium with dilute HCl or NaOH to different pH (1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5 , 10.0, ...

Embodiment 3

[0092] Example 3 Application of Streptomyces cocoa FN to the prevention and treatment of fungal diseases of fruits and vegetables

[0093] Determination of the effect of Streptomyces cocoa FN on apple anthracnose leaf blight, apple leaf spot, apple ring spot, apple rot, apple root rot, apple blight rot, cucumber blight, cucumber blight, tomato early blight, Inhibition rate of watermelon wilt, rape sclerotinia and cabbage black spot pathogens. Draw a straight line through the center of the plate on the back of the PDA plate with a diameter of 90 mm, inoculate 7 mm of probiotic bacteria cake at two points 25 mm away from the center on the line, and inoculate 7 mm of blank PDA medium on the control plate. Inoculate the center of the PDA plate with a 7mm bacterial cake of pathogenic bacteria, and culture it at 28°C for 5-10 days. The radius of the control pathogenic bacteria and the radius of the treated pathogenic bacteria were counted respectively, and the antibacterial rate was...

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Abstract

The invention discloses a biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables, belongs to the technical field of biocontrol bacterium, and particularly provides Streptomyces cacaoi FN. The preservation number of the biocontrol bacterium is CGMCC NO. 13080. The biocontrol bacterium has the functions of preventing and treating apple tree fungal diseases such as apple anthrax leaf blight and apple alternaria leaf spot and fungal diseases of other fruit trees such as tomato early blight and watermelon fusarium wilt, and can not only completely replace chemical prevention and treatment methods and eliminate potential safety hazard of food, but also has outstanding effects of effectiveness and antibacterial broad spectrum properties of apple tree fungal disease prevention and treatment. Meanwhile, the streptomycete has excellent properties of hereditary stability, broad spectrum bacterium resistance, thermal resistance, acid-base resistance, ultraviolet radiation resistance and the like, and can be adopted as an optimal biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables.

Description

technical field [0001] The invention relates to the technical field of biological bacteria agents, in particular to a biocontrol bacteria for preventing and treating fungal diseases of fruits and vegetables. Background technique [0002] During the planting process, fruits and vegetables are often threatened by bacterial and fungal infections, especially fungal diseases. Common symptoms include necrosis (leaf spots, leaf blight), rot (root rot, fruit rot, etc.), wilting (root rot, etc.) , stem base, and vascular tissue are invaded). Symptoms are mildew (downy mildew, black mold, smoke mold, etc.), powder, rust, granular, sclerotinia, etc. There are different methods for preventing and treating fungal diseases of fruits and vegetables. For example, the invention patent with the application number 201410670233.2 discloses "a fungicidal composition containing cymoxanil and its application", which is characterized in that the use of cymoxazone and cymoxanil The composition is ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/465
CPCA01N63/00A01N63/10C12N1/205C12R2001/465
Inventor 周天惠严立恩李玲娣王江王松艳王灵敏
Owner 陕西枫丹百丽生物科技有限公司
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