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Bacillus subtilis with insect prevention function as well as preparation method and application thereof

A Bacillus subtilis, functional technology, applied in the field of microorganisms, achieves the effects of strong broad-spectrum, improved economic benefits and cost savings

Inactive Publication Date: 2018-11-20
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in terms of biological insect control, related technologies have not yet involved

Method used

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  • Bacillus subtilis with insect prevention function as well as preparation method and application thereof
  • Bacillus subtilis with insect prevention function as well as preparation method and application thereof
  • Bacillus subtilis with insect prevention function as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The preparation method of bacillus subtilis WSWFJ-15 is carried out according to the following steps:

[0026] (1) Strain activation: Activate the Bacillus strain WSWFJ-15 stored at -80°C on LB solid medium for 12-16 hours, pick a single colony on LB solid medium, and culture at 35°C for 1-2 days , to obtain activated strains.

[0027] (2) Seed solution preparation: use a sterile inoculation loop to scrape a loop of the activated bacterial strain in step (1) and inoculate it into 50ml LB liquid medium, and cultivate it for 24h at a temperature of 35°C and a rotation speed of 160rpm to obtain a seed solution .

[0028] (3) Fermentation liquid preparation: according to the ratio of bacterial species and culture liquid volume ratio of about 1:120, insert the seed liquid obtained in step (2) into the fermentation material culture medium of the workshop, at a temperature of 37°C (can float up and down) 0.5 DEG C), rotating speed is 120rpm under the condition of cultivating...

Embodiment 1

[0037] Embodiment 1: Utilize WSWFJ-15 to prepare microbial insecticide to kill the effect test of butterfly class pest

[0038] (1) Purpose of the test

[0039] The insecticidal effect of Bacillus subtilis WSWFJ-15 was verified and the effective insecticidal components were determined by spraying test on the larvae of the street tree butterfly in Qinglong Town, Anning.

[0040] (2) Test method

[0041] WSWFJ-15 microbial insecticide is now used to conduct spray tests on different types of pests, and the mortality and death time of each pest are counted. Three treatments were set up in the test: 200 times liquid of microbial insecticide, 500 times liquid of microbial insecticide, 750 times liquid of microbial insecticide, 1000 times liquid of microbial insecticide and clear water control. Each treatment was repeated 3 times, arranged in random blocks, and the spraying amount of each treatment was the same.

[0042] (3) Survey and statistics

[0043]Investigate the insect po...

Embodiment 2

[0050] Embodiment 2: application WSWFJ-15 prepares biopesticide to the killing effect test of pomegranate lampworm larvae

[0051] (1) Purpose of the test

[0052] The anti-insect effect of Bacillus subtilis WSWFJ-15 was verified by spraying test on the larvae of pomegranate moth in Qinglong Town Microbial Fermentation Engineering Research Center Co., Ltd., Anning.

[0053] (2) Test method

[0054] WSWFJ-15 is now used to prepare microbial insecticides to conduct spray tests on the larvae of the lamp moth, and the mortality and death time of each pest are counted. Three treatments were set up in the test: 200 times liquid of microbial insecticide, 500 times liquid of microbial insecticide, 750 times liquid of microbial insecticide, 1000 times liquid of microbial insecticide and clear water control. Each treatment was repeated 3 times and arranged in random blocks. The amount of spraying was the same for each treatment.

[0055] (3) Survey and statistics

[0056] Investiga...

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Abstract

The invention discloses a Bacillus subtilis with an insect prevention function as well as a preparation method and application thereof. The Bacillus subtilisis numbered WSWFJ-15, and has a preservation registration number of CGMCC No. 15855. The Bacillus subtilis is cultured and prepared into microbial insecticidal bacteria liquid or a microbial insecticidal fertilizer. The Bacillus subtilis can be used as a microbial insecticide after fermentation, can be added to fertilizers to prepare a fertilizer product with the insect prevention function for use, has a good insecticidal effect and a strong broad-spectrum property, and does not need to apply multiple varieties for multiple times, thereby saving costs and improving the economic efficiency.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis with an insect-proof function and a preparation method and application thereof. Background technique [0002] Since the 1940s, humans have used pesticides to eliminate insects and weeds, and they have recovered about 15% of the total agricultural output loss every year. However, due to the long-term abuse of pesticides, the harmful substances in the environment have greatly increased, endangering ecology and human beings, and forming pesticide pollution. The pesticides causing pollution are mainly organochlorine pesticides, metal preparations containing lead, arsenic, mercury and other substances, and some specific herbicides. [0003] Most pesticides are poisonous to humans and animals, and can cause acute poisoning and death after a large amount of exposure or accidental ingestion. According to the report of the World Health Organization, due to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P7/04C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 赵崇钧王斌张琪何月秋尚海丽莫夏明李绍董
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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