144results about How to "High broad spectrum" patented technology

A hydrophilic composite immobilized microbe carrier is preapred from foam polyurethane, polyisocyanate, MLZ-type high-molecular water absorbent and activated carbon. Its advantages are large specific surface area, macropore permeability, high hydrophilicity, reactivity and adsorbability, excellent affinity to microbe, and high stability.

Disclosed is a chemistry-microorganism combination restoration method of polycyclic aromatic hydrocarbons and heavy metal composite contaminated site soil. The method comprises steps of (1), preparing soil particles and air drying and grinding contaminated site soil; (2), setting a restoration system, adding methyl beta cyclodextrin leacheate to a blender; (3), adjusting the reaction temperature to be 50+ / -2 DEG C; (4), adjusting an ultrasonic emission device to 35kHz for ultrasound of 30 minutes; (5) conducting continuous leaching, and repeating the continuous leaching for 3 times; and (6) conducting depth restoration of degrading bacteria, and inoculating polycyclic aromatic hydrocarbons degrading bacteria to soil which is subjected to continuous leaching restoration, adjusting the N / P to be 10:1 and conducting degradation for 90-140 days continuously. By the aid of the method, the restoration of the polycyclic aromatic hydrocarbons and heavy metal contaminated site soil generated by enterprises such as transferred coking plants, steel mills and power plants has wide application prospects.

The invention relates to an in-vitro detection reagent for clinical diagnosis, particularly a reagent for determining metabolic disorder in a human body, particularly a p-hydroxy phenylalanine urine detection reagent and a preparation method thereof. The urine detection reagent is prepared by respectively dissolving Hg2<+>, Hg<2+>, Ni<2+> and alpha-nitroso-beta-naphthol in corresponding acid solutions and proportionally mixing. The mercuric sulfate is dissolved in a sulfuric acid solution as a donor of Hg<2+>, the mercurous nitrate is dissolved in a nitric acid solution as a donor of Hg2<+>, the nickel sulfate is dissolved in distilled water as a donor of Ni<2+>, and the alpha-nitroso-beta-naphthol is dissolved in a nitric acid solution. The invention overcomes the defects in the prior art, and provides a new-formula metabolic disorder quick urinalysis reagent which has the advantages of high stability, high sensitivity and wide application range.

A method for treating wastewater in high-plateau and high-cold area, the wastewater and garbage leakage liquid being discharged through processes of physicochemical, biological and ecological steps. In the biological steps, carrier biological reaction device is employed, and by using immobilization technology of microporous biological carrier (WZT) and microorganism to improve the treating effect. In the ecological step, the artificial ecological wetland is employed. The method can treat wastewater especially wastewater in high-plateau and high-cold area with distinct effect because the critical technology of carrier biological reaction device and artificial ecological wetland can suit the climatic condition in high-plateau and high-cold area and it combines the physicochemical, biological and ecological steps organically, the wastewater treated can all reach the I class emission standard of country second category watershed.

The invention belongs to the technical field of soil microbial restoration, and particularly relates to a microbial restoration agent for heavy metal contaminated soil and a restoration method. The restoration method mainly comprises the two steps of preparation of the microbial restoration agent and spraying restoration of the soil. Treatment is conducted on the metal contaminated soil by adopting the microbial restoration agent for the heavy metal contaminated soil and the restoration method, the content of heavy metal in the soil can be rapidly reduced, and the restoration effect is good. The restoration method is simple in operation and low in cost, the restoration method is low in project investment, the dosage of the microbial restoration agent can be adjusted according to practical conditions, when a plurality of metal elements exist in the contaminated soil, treatment can be conducted by mixing and matching different thalli to prepare a microbial restoration agent according to needs. The soil fertility is not damaged and indigenous microorganisms are not harmed when restoration is conducted on the soil by adopting the restoration method, secondary pollution does not exist, the microbial restoration agent and the restoration method are safe and environmentally friendly, and suitable for restoration of a large area of heavy metal contaminated soil, is good in practical application effect, and can be used in a large scale.

The invention relates to an external detection reagent for clinic diagnosis, particularly to a hydroxyphenylalanine urine detection reagent which is a determination reagent for metabolic disorder in the body and its preparation method. The urine detection reagent is prepared by respectively dissolving Hg2<+>, Hg<2+>, Ni<2+> and Cd<2+> in corresponding acid solutions and mixing in proportion. The mercuric sulfate as a donor of Hg<2+> is dissolved in a sulfuric acid solution, the mercurous nitrate as a donor of Hg2<+> is dissolved in a nitric acid solution, the nickel sulfate as a donor of Ni<2+> is dissolved in distilled water, and the cadmium sulfate as a donor of Cd<2+> is dissolved in a nitric acid solution. The invention overcomes the defects and disadvantages existing in the prior art, and the detection reagent is a urine analysis reagent for metabolic disorder with novel formula and has high stability, sensitivity and wide application range.

The invention belongs to the field of molecular biological gene engineering, and particularly relates to a double-control double-regulation prokaryotic expression vector system and a construction method and application thereof. Two expression vectors finish expression of a target gene in the vector system, wherein one expression vector is a main expression vector, and the target gene is cloned on the expression vector; the other expression vector is an auxiliary expression vector, and the auxiliary expression vector controls a switch for regulating the promoter of the main expression vector; the main expression vector contains an SP6 promoter and a lactose regulating gene; the auxiliary expression vector contains an arab promoter, an araC regulating gene and an SP6RNA polymerase gene; and the target gene expressed by the main expression vector and the auxiliary expression vector are controlled and / or regulated by an inducer.

The invention discloses a chemistry-plant combined remediation method of fluoride-type combined pollution site soil. The chemistry-plant combined remediation method of fluoride-type combined pollution site soil comprises the following steps: firstly, crushing and screening pollution soil particles; then placing the soil particles into a soil multielement ex-situ remediation device, adding mixed eluant, and carrying out continuous ex-situ synergism elution by adopting the mode of ultrasonic intensification and warming treatment synchronously; then inoculating purslane for eluted soil, and applying a nutritious source buffer liquid equal to 30%-40% of soil mass; culturing and growing artificially, and completing the remediation. Perfluorinated compounds, polycyclic aromatic hydrocarbon and multiple types of heavy metals in site soil can be eluted efficiently and synchronously; purslane inoculation and outer source nutritional substance adding are beneficial to the deep degradation by combining with residual state polycyclic aromatic hydrocarbon after soil elution, and are also beneficial to the recovery of microorganism ecological function universality and stability of the pollution soil environment.

A broad-spectrum deemulsifier for crude oil contains the polymer of polyol, ethylene oxide and propylene oxide (20-50 wt.%), the polymen of polyamine, ethylene oxide and propylene oxide (20-50), pour depressor (10-20) and solvent (15-25). Its advantages are broad spectrum for more than 10 kinds of crude oils, and low dosage (5-10 ppm).

The invention relates to the technical field of immunotherapy of infectious diseases, in particular to recombinant gene construction of chimeric antigen receptor (CAR) for treating HIV (human immunodeficiency virus) infection and an application of the CAR. A single-chain antibody (ScFv) can identify gp120 of surfaces of HIV infected cells through series connection of antibody light chain and heavy chain variable zones of the gp120 of the surfaces of the HIV infected cells; the ScFv is prepared into the CAR, CAR coding genes are shifted to plasimid vectors, and lentiviral vectors shifted with the CAR coding genes are transduced to CD8+T lymphocytes. The CD8+T lymphocytes are discovered to be remarkable in restraining and killing activities of the HIV infected cells in both in-vitro and in-vivo experiments and can serve as active ingredients to prepare anti-HIV infection drugs, and good application prospect is achieved.

The invention relates to an ozone pre-oxidation and laccase advanced water treatment method, which comprises the following steps of: mixing waste water, ozone and sodium percarbonate in a mixer; adding the mixture in an ozone reactor device for stirred reaction; making the mixture enter a tertiary sedimentation basin for solid-liquid separation; adding the separated supernate and laccase into a laccase reaction tank, and fully mixing the laccase with the waste water for reaction; mixing the diluted mediator and the effluent from the laccase reaction tank, and performing flocculent settling solid-liquid separation on the mixture in a final sedimentation basin; and performing anaerobic and aerobic biochemical treatment on the effluent. The method overcomes the defects of over-high cost, low efficiency of enzyme treatment, high enzyme consumption, difficult sedimentation and separation of enzyme per se, and the like. The method only makes two processing units of ozone pre-oxidation and laccase-mediator catalytic polymerization achieve good mutual acceleration and gradient treatment effects, accelerates decomposition of O3 to make micro-flocculation effect of the ozone pre-oxidation in the optimized pH value range, is favorable for reducing the coagulant consumption, can recycle the waste water, and has scale application.

The invention discloses an analysis device based on an electrochemical luminescence technology and double-pole electrode configuration. The analysis device comprises a mini-type dark room for storing a reaction reagent and having a reaction and a liquid storage tank for storing detection objects and having an oxidization-reduction reaction, wherein a double-pole electrode and an electric signal applying unit are arranged between the mini-type dark room and the liquid storage tank respectively; one end of the double-pole electrode is packaged in an inner cavity at the bottom of the mini-type dark room and the other end of the double-pole electrode stretches into the liquid storage tank; and meanwhile, a positive pole of the electric signal applying unit stretches into the liquid storage tank through a driving electrode and a negative pole of the electric signal applying unit is connected with the top of the mini-type dark room through a driving electrode. According to the analysis device based on the electrochemical luminescence technology and the double-pole electrode configuration, the stored detection objects are linked with signal molecules, so that the oxidization-reduction reaction in the liquid storage tank is transmitted into the mini-type dark room in an electron form, a light signal is generated and a corresponding electrochemical luminescence signal is realized, so that the sensitivity and reliability of detection are extremely improved.

The invention discloses a nano-porous copper-silver bimetal / bimetal oxide and a preparation method and application of the nano-porous copper-silver bimetal / bimetal oxide. Nano-porous copper-silver bimetal obtained after an amorphous matrix is dealloyed is used as an anode for electrochemical oxidation, the finally-obtained bimetal oxide is loaded to the surface of the nano-porous copper-silver bimetal in a nanowire shape, and the amorphous matrix is the CuxZryAgz alloy ingredient, wherein x, y and z are atomic percents, x is larger than or equal to 35 and is smaller than or equal to 45, y is larger than or equal to 35 and is smaller than or equal to 45, z is larger than or equal to 10 and is smaller than or equal to 30, and the sum of x, y and z is 100. The preparation process is simpler, the mechanical integrity is better, cyclic reuse can be achieved, bar products are easy to recycle, and secondary pollution of powder products is avoided.

The invention discloses a preparation method of a series of chitosan polyamine derivative-silver supported nanometer titanium dioxide composite material. The composite material comprises chitosan polyamine derivatives and silver supported nanometer titanium dioxide. The preparation method of the composite material comprises the following steps: preparing the series of the chitosan polyamine derivatives; suspending the derivatives in a certain amount of an organic solvent which can be an alcohol, acetonitrile, DMF, or DMSO, or in a mixed system of the organic solvent and water; stirring; heating; adding the prepared silver supported nanometer titanium dioxide; reacting for a period of time; carrying out reduced pressure filtration; washing; and drying to obtain the chitosan polyamine derivative-silver supported nanometer titanium dioxide composite material. The composite material has the advantages of nontoxicity, low price, biodegradability and the like of chitosan natural substances, and also has the advantages of high stability and efficient antibacterial property of nanometer titanium dioxide and the advantages comprising the efficient antibacterial property and the like of silver ions, so the composite material has large application values in the antibacterial property and the heavy metal ion removal, and has superior performances incomparable by single materials.

The invention discloses a compound demulsifier for heavy inferior crude oil. The compound demulsifier can improve the emulsifying property of the oil-water interface relatively well for O / W type heavy inferior crude oil and thick oil so as to realize a relatively good dehydration effect with relatively small dosage; and moreover, the dehydration efficiency is obviously improved, and a little oil is carried by the discharged water. The composition and content of the compound demulsifier are as shown in the specification.

The present invention belongs to the field of fire detecting and warning technology. The smoke concentration detector includes air filter, semiconductor laser, laser beam space correcting lens, laser beam space filtering diaphragm, detecting chamber, detecting chamber rack, smoke scattering detector, laser power detector, extinction mechanism, reflector and casing. All the parts are installed inside the casing except the air filter inserted to the air inlet of the casing.

The invention discloses a high-efficiency compound demulsifier for heavy and poor crude oil. The high-efficiency compound demulsifier can improve the emulsification performance of oil-water interfaces of oil / water (O / W) heavy and poor crude oil and thickened oil, so that high dehydration effect can be achieved in a small using amount and the dehydration efficiency is greatly improved and the discharged water carries a small amount of oil. The compound demulsifier comprises the following components in percentage by mass: 30 to 50 percent of block polyether polymer demulsifier, 3 to 5 percent of novel organosilicon polymer demulsifier, 0.5 to 5 percent of demulsifier additive, and 40 to 60 percent of solvent.

The invention belongs to microbial strains and preparation methods of the microbial strains, and particularly relates to Escherichia coli and a preparation method of the Escherichia coli. A cloned iss gene segment in outer membrane protein of Escherichia coli pathogenic bacteria of a coded bird is regrouped in an expression plasmid pEGX-6p-1 after BamHI and SalI double enzyme digestion, and a pronucleus expression plasmid pEGX-6p-1 / iss is built; the pronucleus expression plasmid pEGX-6p-1 / iss is transformed into Escherichia coli BL21-DE3, and regrouped Escherichia coli BL21-DE3 / pEGX-6p-1 / iss is built. The technical problem that the vaccine broad-spectrum performance is poor in the prior art is solved. The Escherichia coli has the advantages of being good in broad spectrum performance, good in immune effect, wide in industrialized development prospect and the like.

The invention discloses a low-temperature demulsifier for a crude oil emulsion and a production method thereof. The low-temperature demulsifier comprises the following components: 35-65% of functional demulsifier, 1-10% of low-temperature complexing agent, 0.5-5% of additive and 35-65% of organic solvent. The production method of the low-temperature demulsifier comprises the step of compositely preparing with a reaction kettle with a clamp sleeve. The low-temperature demulsifier has wide suitable crude oil types and has better application performance compared with the traditional low-temperature demulsifier.

The invention discloses a multifunctional traditional Chinese medicine organic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent and a preparation method thereof. Under the action of EDC / NHS, a traditional Chinese medicine organic acid reacts with chitosan quaternary ammonium salt molecules to synthesize the traditional Chinese medicine organic acid-chitosan quaternary ammonium salt broad-spectrum antibacterial agent with antibacterial, anti-inflammatory and antioxidant properties. The method is simple and convenient in steps, low in investment, high in output and suitable for large-scale production and use. Through the synergistic effect of the traditional Chinese medicine organic acid and the chitosan quaternary ammonium salt, the broad-spectrum antibacterial property superior to that of the chitosan quaternary ammonium salt is obtained; through parameter optimization, the obtained traditional Chinese medicine organic acid-chitosan quaternary ammonium salt antibacterial agent has no cytotoxicity; on the other hand, the traditional Chinese medicine organic acid endows the antibacterial agent with good anti-inflammatory performance and oxidation resistance; and due to no cytotoxicity and multi-functionalization, the traditional Chinese medicine organic acid-chitosan quaternary ammonium salt broad-spectrum antibacterial agent has a wider application prospect.

The invention provides a chimeric antigen receptor, a NKG2D CAR-NK cell expressing the chimeric antigen receptor, a preparation method and an application thereof. The chimeric antigen receptor comprises an antigen binding domain, a transmembrane domain, and a costimulatory signaling domain, and the antigen binding domain is capable of specifically binding to a tumor specific antigen NKG2D ligand and activating NK cells through the transmembrane domain and the costimulatory signaling domain. The CAR-NK cell uses the NKG2D ligand as a target antigen and specifically uses the NKG2D CAR-NK cells to kill tumor cells. The cell can be used as a therapeutic drug for tumor diseases for the treatment of tumors with high expression of the ligand of NKG2D molecules, and provides a novel method for tumor prevention and treatment.

The invention provides graphene composite photocatalytic aerogel with a hierarchical porous structure and a preparation method thereof. The graphene composite photocatalytic aerogel has a lamellar structure and a three-dimensional structure and also has the hierarchical porous structure with a macropore, a mesopore and a micropore. An adsorption effect of graphene aerogel is combined with a photocatalysis effect of titanium dioxide, pollutants adsorbed to pores in the graphene aerogel and on the surface of the graphene aerogel are rapidly degraded by utilizing a photocatalysis effect of nano TiO2 and the graphene aerogel is generated in situ, so that the adsorption saturation time of the graphene aerogel is prolonged and the decontamination effect is greatly improved. The hierarchical porous structure in the composite graphene aerogel can be relatively conveniently regulated and controlled according to actual application requirements through changing types and adding amounts of added active carbon fibers, so that the universality that the graphene composite photocatalytic aerogel is applicable to different pollutants is improved. A whole set of synthesis process is simple and stirring is not needed in a chemical reduction in-situ self-assembling process; reaction conditions are moderate.

The invention relates to the technical field of pharmaceuticals, and provides a substituted aryl tetracyclic antifungal compound and pharmaceutically acceptable salts. The substituted aryl tetracyclic antifungal compound is as shown in a structural formula of the description. The invention further provides a preparation method of the compound and an application of the compound in preparing antifungal medicaments.

The invention relates to a preparation method of a layered unsaturated silver-zinc-copper-loaded composite antibacterial antiviral material. The preparation method comprises the following steps: weighing a silver salt, a zinc salt, a copper salt, a modifier, a complexing agent and a solvent, and modifying and complexing metal ions by adopting ultrasonic-assisted high-speed shearing and stirring toprepare metastable complex silver-zinc-copper composite ions; then carrying out stirring and dispersing, adsorbing the metastable complex silver-zinc-copper composite ions to interlayers of a two-dimensional nanometer layered material through unsaturated adsorption, then adding the material into a supercritical reaction kettle, and pressing carbon dioxide into the reaction kettle by using a high-pressure pump; and controlling the pressure and the temperature of the reaction kettle, subjecting a supercritical fluid to rapid pressure relief through a nozzle, and successively conducting centrifugation, drying and crushing to obtain the layered unsaturated silver-zinc-copper-loaded composite antibacterial antiviral material. The composite antibacterial antiviral material prepared by the method has the advantages of good heat resistance, high safety and the like, can be used for preparing antibacterial and antiviral polyester, nylon and other fibers, can be blended with tencel, combed cotton and the like, and is widely applicable to the fields of underwear, sports coats, home textiles and the like, comfortable in fabric hand feeling and strong in antibacterial and antiviral functions.

The invention relates to a polyethylene composite antibacterial coating containing nanometer silver particles and a preparation method thereof. The polyethylene composite antibacterial coating containing nanometer silver particles comprises polyethylene and nanometer silver particles uniformly distributed in polyethylene, wherein the mass fraction of the nanometer silver particles in the polyethylene composite antibacterial coating containing the nanometer silver particles is no less than 0.0714 wt%, preferably 0.0714 to 0.714 wt%. The preparation method of the invention has the advantages ofeasy operation, low spraying temperature, high efficiency, etc.; since a thermal spraying process is not utilized, the degradation and oxidation of polyethylene are effectively prevented, and the physical and chemical performance of the polymer is maintained unchanged. The polyethylene composite antibacterial coating containing the nanometer silver particles is applicable to the industries of foodpackaging, medical apparatuses and instruments, space equipment materials and the like. The coating can effectively reduce the consumption of Ag.

The invention belongs to the technical field of medicaments and provides a substituted benzothiazole compound and a pharmaceutically acceptable salt thereof. The structural formula of the substituted benzothiazole compound is as shown in formula I, wherein in formula I, R is a substituted naphthalene ring or a substituted aromatic heterocycle. The invention also provides the applications of the substituted benzothiazole compound and the pharmaceutically acceptable salt thereof to the preparation of medicaments for resisting fungi, tumors, viruses, high blood pressure and thrombus and topoisomerase inhibitors.

The invention relates to the technical field of high polymer materials, in particular to a food-grade antibacterial antistatic PLA material and a preparation method. The PLA material comprises the following substances in parts by weight: 50-70 parts of PLA resin, 10-20 parts of PBAT, 5-20 parts of filling mineral powder, 0.5-3 parts of an antistatic agent, 6-20 parts of an antibacterial agent, 3-5parts of a coupling agent and 1-2 parts of a lubricant. Compared with the prior art, the PLA material has the following beneficial effects that the used antibacterial agent is an inorganic-organic compounded antibacterial agent, so that the broad spectrum of the antibacterial material is further improved, the antibacterial effect is better, the cost is reduced, and the PLA material conforms to FDA, ROHS and REACH certification and tests and can be used for food and medical products. The used antistatic agent is sulfonate prepared by a distillation method, has good compatibility and no precipitate, and also has an excellent antistatic and dust-removing performance in a low-humidity environment.

The invention discloses a salmonella molecular detection kit based on bcfD genes and a non-diagnostic detection method. The kit comprises (1) 10* Lamp buffer; (2) 8000U / mL BstDNA polymerase; (3) 10mM dNTPs; (4) a Lamp primer group: outer primer 5pmol / micro liter, inner primer 40pmol / micro liter, and annular primer 20pmol / micro-liter; (5) double distilled water; (6) fluorescent dye: 10*SYBR Green I; (7) positive control: salmonella standard strain genome DNA; and (8) negative control: double distilled water. The LAMP detection method has the advantages of rapidness, high efficiency, simplicity in operation, high specificity, high sensitivity, broad-spectrum property, applicability to in-situ detection and the like; no complicated instrument is needed; novel technical support is provided for the detection of salmonella; the salmonella molecular detection kit can be used for screening and detecting salmonella in animal husbandry production units, basic medical sanitary units, entry-exit and different disease control centers and is wide in market prospect, good in economic benefit and social benefit and wide in application range.

The invention discloses compound oil phase dispersing emulsion splitter which uses polysilicone emulsion splitter as strengthening agent and is produced by preparing the traditional non-siliceous emulsion splitter, additive and mixed solvent. The compound oil phase dispersing emulsion splitter effectively increases the application effect of the traditional non-siliceous emulsion splitter by using the special function of the polysilicone emulsion splitter and the joint function of the traditional non-siliceous emulsion splitter and has the functions of high emulsion splitting efficiency, less consumption, high comprehensive performance price ratio, wide adaptability to different crude oils and low temperature emulsion splitting.