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Microbial restoration agent for heavy metal contaminated soil and restoration method

A technology for microbial remediation and contaminated soil, applied in the restoration of contaminated soil, soil conditioning materials, chemical instruments and methods, etc., can solve the problems of long duration of microbial remediation, inability to remove heavy metals, competition of indigenous strains, etc., and achieve sustainable remediation Short time, high cost performance, no secondary pollution effect

Active Publication Date: 2017-12-01
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial remediation technology for heavy metal-contaminated soil has certain limitations, such as long duration of microbial remediation, slow effect, single function, general formation of complexes to reduce the harm of heavy metals, and heavy metals cannot be removed from the soil. In addition, the microbes are small, and it is difficult to separate them from the soil, and there are problems such as competition with the indigenous strains at the restoration site.

Method used

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  • Microbial restoration agent for heavy metal contaminated soil and restoration method
  • Microbial restoration agent for heavy metal contaminated soil and restoration method
  • Microbial restoration agent for heavy metal contaminated soil and restoration method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A microbial remediation method for heavy metal contaminated soil, characterized in that it comprises the following steps:

[0029] (1) Preparation of microbial remediation agent: culture Aspergillus tabingensis on sucrose basal medium for 5 days and collect mycelial balls, add 721 type cation exchange resin at a ratio of 30g (resin) / g (dry mycelial balls), shake at 150 rpm for 2 hours , centrifuged at 4000 rpm for 20 minutes, and the supernatant was concentrated and then freeze-dried for 24 hours to obtain an extracellular polymer (EPS) powder, which is a microbial remediation agent; bury the infiltration layer and water collection device under the soil polluted by heavy metals , and set up the pump;

[0030] (2) Mix the microbial remediation agent with water, the concentration of the remediation agent (ratio of the mass of the remediation agent to the volume of water) W / V is 10%, to obtain a mixed solution, and use the mixed solution for the polluted environment contai...

Embodiment 2

[0035] A microbial remediation method for heavy metal contaminated soil, characterized in that it comprises the following steps:

[0036] (1) Preparation of microbial remediation agent: Use food production wastewater to cultivate sulfate-reducing bacteria and Aspergillus flavus to a stable stage, collect the bacteria, add deionized water at a ratio of 10g / L (dry weight of bacteria), under 40KHZ conditions Ultrasound for 50 minutes, followed by centrifugation at 4000rpm for 25 minutes, the resulting supernatant is the extracellular polymer (EPS) solution of the two bacteria, which is the microbial remediation agent; bury the infiltration layer and catch water under the soil polluted by heavy metals device, and set up the pump;

[0037] (2) Dilute the concentration of the microbial remediation agent with water to 0.001% to obtain a mixed solution, and use the mixed solution for drip irrigation on lead-contaminated soil. The volume of the mixed solution used is 10 times the volum...

Embodiment 3

[0041] (1) Preparation of microbial remediation agent:

[0042] (1) Extraction of EPS: grow Aspergillus tabinensis to the stable stage, collect mycelium balls, and add 732 Type cation exchange resin and deionized water, then stirred at 150rpm for 4 hours, collected mycelial balls and supernatant, and centrifuged at 4000rpm for 20 minutes, the obtained supernatant was crude extracellular polymer (EPS) solution, crude extracellular The polymer (EPS) solution is put into a 3500Da dialysis bag, and the dialysis bag is placed in high-purity water for dialysis. During the dialysis process, 200rpm magnetic stirring is used, and the water is changed every 4 hours. The sperm extracellular polymer (EPS) solution is obtained by dialysis for 24 hours. ; After the dialysis is completed, use 20000Da polyethylene glycol to concentrate until the volume becomes 1% of the original, and freeze-dry to obtain EPS powder;

[0043] (2) Carrier pretreatment and loading:

[0044] ① GO (graphene oxid...

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Abstract

The invention belongs to the technical field of soil microbial restoration, and particularly relates to a microbial restoration agent for heavy metal contaminated soil and a restoration method. The restoration method mainly comprises the two steps of preparation of the microbial restoration agent and spraying restoration of the soil. Treatment is conducted on the metal contaminated soil by adopting the microbial restoration agent for the heavy metal contaminated soil and the restoration method, the content of heavy metal in the soil can be rapidly reduced, and the restoration effect is good. The restoration method is simple in operation and low in cost, the restoration method is low in project investment, the dosage of the microbial restoration agent can be adjusted according to practical conditions, when a plurality of metal elements exist in the contaminated soil, treatment can be conducted by mixing and matching different thalli to prepare a microbial restoration agent according to needs. The soil fertility is not damaged and indigenous microorganisms are not harmed when restoration is conducted on the soil by adopting the restoration method, secondary pollution does not exist, the microbial restoration agent and the restoration method are safe and environmentally friendly, and suitable for restoration of a large area of heavy metal contaminated soil, is good in practical application effect, and can be used in a large scale.

Description

technical field [0001] The invention belongs to the technical field of soil microbial remediation, and in particular relates to a microbial remediation agent and a remediation method for heavy metal polluted soil. Background technique [0002] Soil is one of the main natural resources that human beings rely on for survival. It is an important place for the exchange and circulation of materials and energy in the earth's ecosystem. reversed. From April 2005 to December 2013, my country carried out the first national soil pollution survey. The survey results show that the overall situation of the soil environment in the country is not optimistic. Some areas have serious soil pollution, the soil environment quality of cultivated land is worrying, and the soil environment problems of industrial and mining wasteland are prominent. The total point exceeding standard rate of national soil is 16.1%. From the perspective of pollutant exceeding standard, the point exceeding standard ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C09K17/32
CPCB09C1/105B09C2101/00C09K17/32
Inventor 刘幽燕卢宇浩程宁唐爱星李青云
Owner GUANGXI UNIV
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