282results about How to "Simple sampling" patented technology

A blood sampling device useful for collecting a blood sample from a separate vascular access device is described herein. The blood sampling device includes a body shaped and sized for partial insertion into a separate vascular access device. The body includes a reservoir defined within the body, which has an internal volume sufficient to contain enough blood for use in a diagnostic blood test. The body also includes a gas permeable vent disposed on the body, in which the gas permeable vent is in gaseous communication with the reservoir. When connected to a separate vascular access device the blood sampling device collects a blood sample as blood flows into the reservoir from the separate vascular access device and as gases pass out the reservoir via the gas permeable vent.

The invention discloses a portable shallow soft soil original state sampling device and a sampling method. A solution is provided for solving the problems that soft soil with a high water content is likely to fall along the inner wall of a sampler, an original state soil column is easily disturbed in repairing, separating and post-treating processes, and the inner cavity of the sampler is difficult to clean and the like. The sampling device comprises the sampler, a positioner and a separator. Smooth sampling is realized through a sampling rod, a steering base, a force transfer base and a sampling barrel; a negative pressure is generated in the inner cavity of the force transfer base by virtue of a piston and a baffle plate, so that a soil sample is prevented from falling. By opening out a liner pipe and the separator, the soil column is repaired, separated and packaged. The method comprises the following steps: removing miscellaneous soil in the surface layer, assembling the sampling device, and pressing the sampler into a specific depth; then lifting up the sampling rod to close the inner cavity of the force transfer seat; and screwing the sampler out, detaching the sampling barrel, pushing the liner pipe out by using the separator and performing packaging with a cover. The device disclosed by the invention is simple in structure, convenient to operate, small in sampling disturbance and high in efficiency.

The invention discloses a novel coronavirus detection kit. The kit comprises a bottom plate, and a sample pad, a colloidal gold pad, an NC membrane and a water absorption pad which are fixed on the bottom plate, wherein a detection line and a quality control line are arranged on the NC membrane, an antigen coated on the colloidal gold pad is a mixed antigen protein composed of N protein marked bycolloidal gold and S-RBD protein marked by colloidal gold, and the detection line is a mouse anti-human IgM antibody and mouse anti-human IgG antibody detection line. The detection kit provided by theinvention takes N protein and S-RBD protein as antigens, can improve the sensitivity of COVID-19 preliminary detection, and is suitable for quick screening of large-scale crowds.

The invention discloses a soil leaching liquor collection method under natural conditions, which belongs to the research field of environmental science, soil science and plant nutrition. The soil leaching liquor collection method of the invention mainly includes the following steps: a soil profile in the vertical direction is obtained and the dug-out soil is piled up layer by layer according to depth; a horizontal cave is dug based on the target depth of the soil profile and the upper surface of the cave is guaranteed to be level; a leaching plate is inserted into the cave and slurry modulated out of soil of the soil layer of the target depth is placed into the leaching plate; the dug-out soil is backfilled layer by layer and then compacted layer by layer, moreover, in order to recover the raw condition of the backfilled soil as possible, the backfilled soil is irrigated for a plurality of times. The method of the invention can realize collecting and monitoring the soil leaching liquor under natural conditions, in particular is suitable for monitoring the influence caused by the soil leaching liquor of such soil solute as nitrogen, phosphorus and pesticide, etc., on the groundwater.

The invention discloses a method for detecting diversity of fish species on the basis of an environmental DNA technology. The method comprises the following steps of (1) conducting sampling; (2) extracting eDNA; (3) performing PCR amplification; (4) recovering and purifying target fragments and constructing a target fragment library; (5) conducting OTU clustering; (6) creating a fish comparison database; (7) conducting comparison analysis on representative sequences of OTUs with data information of the created fish database to determine the species diversity composition of fishes in a to-be-detected water area. According to the method, there is no need to catch the fishes, sampling is simple, and the fish bodies cannot be damaged; detection is convenient, and there is no need to conduct fish appearance identification; detection is simple and fast, the precision is high, and a detection result is accurate.

The invention discloses multiple real-time quantitative PCR primer, probe and a detection method for identifying viral pathogens relevant to fevers with eruption syndromes as infection diseases, which is used for carrying out multiple real-time fluorescent quantitative PCR detection on varicella-herpes zoster viruses, human small DNA (Deoxyribonucleic Acid) viruses B19, enteroviruses (enteroviruses 71 type and coxsackie viruses A16 type), dengue viruses, rubella viruses and measles viruses. The invention can simultaneously carry out qualitative or quantitative detection on eight kinds of human viruses in various types of samples by multiple double-tubes PCR. The detection method has the advantages of simple operation, short time consumption, high sensitivity and strong specificity, is suitable for field detection, early diagnosis, epidemics detection and research and the like, and takes the actions of assistance and identification diagnosis on the fevers with eruption syndromes.

The invention discloses a method for detecting pathogens of infectious diseases, which possibly exist in a biological sample. The pathogens of the infectious diseases comprise avian influenza virus H5 subtype, avian influenza virus H7 subtype, SARS coronavirus, hanta virus, plague yersinia pestis and bacillus anthracis. The method comprises amplifying a nucleic acid fragment of a biological sample and detection by a probe. The invention also provides a primer for amplification and a probe for detection. The invention also provides a kit including the primer. The method has the advantages of high flexibility, strong specificity, easy operation, wide sample range, the detection for the pathogens of various infectious diseases at the same time and the suitability for early diagnosis of respiratory infectious diseases.

The invention discloses a polyimide containing porphyrin, and aromatic polyimide with better thermal stability is obtained by using aromatic dicarboxylic anhydride with better thermal stability and aromatic organic diamine as basic framework. The preparation method comprises the following step of carrying out condensation reaction on diamino tetraphenyl porphyrin, the aromatic organic diamine and the aromatic dicarboxylic anhydride to synthesize soluble polyimide containing porphyrin, having the characteristics of simple operation, strong controllability and recyclable use of products. The polyimide containing the porphyrin can be used for preparing a corresponding nano fiber film of the polyimide containing the porphyrin by using an electrostatic spinning method, and the nano fiber film is used for detecting explosive steam under an extreme environment, having advantages of high sensitivity and rapid response.

The invention discloses a method for manufacturing customized shoe pads. The method comprises the following steps of: (1) selection of the conventional shoe pad lower die; (2) manufacturing of a plastic shoe pad die: filling a plastic material into a die cavity of the shoe pad lower die, and thus obtaining the plastic shoe pad die; (3) footprint acquisition, in which a customer stands on the plastic shoe pad die and steps on the plastic material to form a concave footprint; (4) processing of a shoe pad upper die: processing and manufacturing an upper die of a shoe pad mold according to the footprint on the plastic shoe pad die; and (5) die assembly. Therefore, the footprint of the customer who naturally stands is acquired by manufacturing the plastic shoe pad die which can be quickly shaped through the conventional shoe pad lower die, a customized shoe pad upper die is manufactured according to a bottom cambered surface of the footprint, shoe pads which are customized by the customer is quickly and accurately manufactured by assembling the shoe pad upper die and the shoe pad lower die, manufacturing and sampling processes are simplified, time consumption is reduced, and the foot type of the customer is not required to be scanned by a precise imager, so the cost of the customized shoe pads is greatly reduced.

The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise chlamydia pneumoniae, haemophilus influenzae, mycoplasma pneumoniae, pneumoniae streptococcus and legionella pneumophila. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.

The invention discloses a coal seam low-temperature fixed-point frozen sampling device and a use method thereof. The coal seam low-temperature fixed-point frozen sampling device comprises a through hole coring bit. The outer wall and the inner wall of the right end of the through hole coring bit are connected with a sampling drill pipe and a frozen low-temperature sample storing device correspondingly. The right end of the sampling drill pipe is connected with a drill pipe adapter. A dry ice cooling layer and a heat-insulation heat-preservation layer are arranged between the sampling drill pipe and the frozen low-temperature sample storing device. According to the sampling method, a drilled hole is constructed to the designed depth, the coal seam low-temperature fixed-point frozen sampling device is put into the bottom of the drilled hole and pushed towards a coal seam, a collected coal sample pushes a round baffle to enter an inner cavity of a low-temperature sample storing barrel and is cooled rapidly, meanwhile, water is supplied into a frozen sealed barrel, the coal sample mixed with water in the frozen sealed barrel is frozen rapidly in a low-temperature environment, dissipation of gas is lowered, a sealing bolt is detached after a drill is retreated, the internal coal sample is rapidly poured out by pushing the round baffle with a metal guide rod, and the coal sample is transferred into a coal sample tank to be sealed and stored. The coal seam low-temperature fixed-point frozen sampling device and the use method thereof realize low-temperature fixed-point frozen sampling, can effectively reduce the loss of gas in the sampling process, and are beneficial to improving the accuracy of the measured gas content of the coal seam.

The invention discloses a soil sampler used for geological detection and belongs to the technical field of geological detection. The soil sampler comprises a drill rod; the bottom of the drill rod isconnected with a sampling cylinder; the bottom of the sampling cylinder is connected with a drill head; a plurality of sampling scrapers are distributed at intervals of equal central angles in the sampling cylinder; a plurality of outlets which are in one-to-one correspondence with the opening surfaces of the sampling scrapers are formed in the cylindrical wall of the sampling cylinder; the outlets are provided with opening-closing mechanisms which can open and close the outlets; a sampling driving mechanism which is used for pushing the sampling scrapers out of the outlets or retracting the sampling scrapers is arranged in the sampling cylinder; the sampling driving mechanism includes a driving shaft; the driving shaft is provided with protruding driving blocks; the number of the protruding driving blocks is equal to the number of the sampling scrapers; the driving shaft servers as the rotation axis of the protruding driving blocks; each protruding driving block is tangent to the backof the scraper corresponding to the protruding driving block; and a reset spring is connected between the back of each sampling scrapper and the driving shaft. According to the soil sampler of the invention, the sampling scrapers can be flexibly pushed out or retracted, and therefore, soil from other soil layers can be prevented from entering the sampling cylinder, and the purity of a sample canbe effectively ensured.

The invention discloses a method for extracting fetus gene information. In the provided method, maternal cell DNA and acellular DNA are separated from a maternal sample, wherein the acellular DNA is from the fetus. Two DNA samples are strictly processed by same reagents and methods and subjected to parallel sequencing in a same sequencing reaction; and the fetus gene information and fetus gene abnormity can be obtained by comparing the sequencing results of two DNA samples. The provided autologous comparing method can be applied to detection of chromosome aneuploidy, and can also be applied to gene detections such as chimera detection, copy number variation detection, single point mutation detection, and the like.

The invention provides a recombinant protein and test paper for detecting a novel coronavirus 2019-nCoV IgA antibody, and a preparation method and application, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein is shown in SEQ ID No.1; the test paper comprises a bottom plate as well as a sample absorption pad, a fluorescent microsphere pad, anitrocellulose membrane and a water absorption pad which are in sequential lap joint and adhesion to the bottom plate; a 2019 novel coronavirus specific antigen marked by fluorescent microspheres is sprayed to the fluorescent microsphere pad; a detection zone and a quality control zone are fixed on the nitrocellulose membrane; an anti-human IgA antibody is sprayed to the detection zone; and a sheep anti-chicken IgY antibody is sprayed to the quality control zone. By detecting the IgA antibody in a saliva sample, the test paper provided by the invention is capable of simply, rapidly and accurate detecting novel coronaviruses, and early-stage detection on the novel coronaviruses can be achieved.

The invention provides an application of Survivin antibody, and particularly relates to an application for preparing an esophageal cancer immunochromatography detecting test strip. The invention also provides an esophageal cancer immunochromatography detecting test strip, comprising a nitric acid fibrin film layer and a glass fiber film layer, wherein, a T test area and a C quality control area are arranged on the nitric acid fibrin film layer, the test area comprises Survivin monoclonal antibody or polyclonal antibody, the quality control area is enveloped with second antibody IgG; the glassfiber film layer is fixed with the Survivin polyclonal antibody or monoclonal antibody which are marked by colloidal gold. The method simultaneously provides a method for detecting the esophageal cancer immunochromatography with the test strip. The method for detecting the esophageal cancer immunochromatography with the test strip which is invented with the monoclonal antibody or the polyclonal antibody has the advantages of non-invasive, simple operation, sensitivity, easy sampling and the like, can fast, directly and qualitatively detect the Survivin in a sample, and can be used for screening esophageal cancers for people in a high incidence area.

The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise EB virus, herpes simplex virus, cytomegalic inclusion disease virus and adenovirus. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.

The invention relates to sampling equipment, in particular to distributed sampling equipment for industrial sewage. The distributed sampling equipment for the industrial sewage aims at achieving the technical purposes that the speed of sampling the sewage is high, and a user conveniently samples the sewage in different positions. The distributed sampling equipment for the industrial sewage comprises a base plate, a bracket, a top plate, a first fixing sleeve, a first sliding rail, a first sliding block, a rack, a first connecting rod, a second connecting rod, a first cylinder body, a first piston and the like; the bracket is arranged at the left side of the top of the base plate, the top plate is connected to the top of the bracket, the first fixing sleeve is connected to the right side ofthe bottom of the top plate, and the first sliding rail is arranged at the left side of the top of the base plate. The distributed sampling equipment for the industrial sewage has the advantages thatthe speed of sampling the sewage is high, and the user conveniently samples the sewage in different positions; through the arrangement of a first hose and a second hose, the user can be farther awayfrom a sewage pool, and the hands are prevented from being stained by the sewage.

The invention discloses a soil leaching liquor in-situ measurement method, which belongs to the research field of environmental science, soil science and plant nutrition. The soil leaching liquor in-situ measurement method of the invention includes the following steps: a soil profile in the vertical direction is obtained and a horizontal cave is dug based on the target depth of the soil profile; a horizontal leaching plate is arranged in the cave to collect the leaching liquor, and the leaching liquor in the leaching plate can automatically flow into a sample bottle through an outlet pipe; soil of the soil layer with a corresponding depth is used for filling the cave; according to the steps, a vertical waterproof wall body is built along the vertical soil profile to ensure that the outlet pipe can pass through the wall body; meanwhile, the soil is backfilled layer by layer and then compacted layer by layer, and moreover, in order to recover the raw condition of the backfilled soil as possible, the backfilled soil is irrigated for a plurality of times. The soil leaching liquor in-situ measurement method of the invention is especially suitable for monitoring the influence caused by the soil leaching liquor of such soil solute as nitrogen, phosphorus and pesticide, etc. to the groundwater; particularly, under the condition of realizing the in situ monitoring, the sampling difficulty is reduced and the sampling process becomes simple and fast.

The invention relates to an automatic mobile cotton bale and cotton sampling device which comprises a base, a mobile guide track, a station rotation table and a working station table, wherein a cotton packaging material cutting mechanism, a cotton bale bundling material shearing mechanism and a cotton sample grasping mechanism are respectively arranged on the working station table; the cutting mechanism comprises a first guide track; a cutter rotating disc which is connected with a cutter base is arranged on the first guide track; a cutter sliding track matched with a cutter holder is arranged on the cutter base; a cutter is arranged on the cutter holder; the cutting mechanism comprises a second guide track; a scissor rotating disc is arranged on the shearing mechanism; the scissor rotating disc is connected with a scissor base; a pair of cotton bale bundling material scissors is arranged on the scissor base. Due to adoption of a cotton shifting mechanism, cotton, of which fiber is cut off when external packaging is opened, can be shifted off, and cotton, of which fiber is not cut off, can be gasped out from a cotton bale to be detected, so that detection accuracy can be improved, and detection errors can be avoided; and due to adoption of multiple functional mechanisms on the working station table, the whole process of sampling cotton and external cotton packaging can be completed by using one piece of equipment.

The invention relates to a drilling and exhausting integrated type geogas sampling device. The drilling and exhausting integrated type geogas sampling device comprises a rod body, a hammering pile, a gas suction pipe, a steering device, a gas nozzle and a sealing device, wherein the hammering pile is fixed at an opening of the rod body; the steering device is mounted at the upper part of the rod body and can rotate relative to the rod body; the gas suction pipe is mounted in the rod body; the upper end of the gas suction pipe is fixedly connected with the middle part of the steering device; the sealing device is mounted between the upper part of the gas suction pipe and the rod body; and the gas nozzle is mounted on an inner wall of the top of the gas suction pipe. In a geogas measurement process, after a sampling hole is formed in soil by using a drilling rod, the rod body does not need to be pulled out and can be directly connected with a gas capturing device and an exhausting barrel to directly sample, so that the occurrence of hole collapsing and the mixing of atmosphere substances are avoided, the smooth development of geogas measuring work is guaranteed and the working quality is also improved; a rotary rod can be used for controlling a movable door and also can be used as a handle so that the drilling rod is easy to pull out; and furthermore, by virtue of the integrated sampling device, a sampling process is simplified, sampling time is shortened and the working efficiency is improved.

The invention relates to a device for sampling secretions from plant roots, comprising a planting pot (1), a bracket (2), a collecting pipe (3), a water tank (4), a switch I (5), a switch II (6) and a sampling bottle (8), wherein the bracket (2) is in the shape of a root and is arranged in the planting pot (1); the collecting pipe (3) is fixedly connected to the bracket (2); an uncovered head face of the collecting pipe (3) is arranged in the planting pot (1) and the other uncovered head face forms a total outlet end to be arranged at the outer end of the bottom of the planting pot (1); an outlet end of the collecting pipe (3) is arranged in the sampling bottle (8) after connected with the switch II (6); the outlet end of the collecting pipe (3) is connected with the water tank (4) after connected with the switch I (5); and the height of the water level of the water tank (4) is equal to that of the plant roots of the planting pot (1). Therefore, the sampling is easier.

The invention relates to the technical field of biologics, and in particular relates to a primer probe and kit for detecting an oncogene of a cervical cancer and a detection method for a non-detection disease purpose. The probe is high in specificity; and the false positive rate is reduced. By the method, quantified detection for nucleic acid to be detected is realized by hybridization, signal magnification and enzymatic chemiluminescence; the magnification factor is determined without a magnification process for increasing an index; and the repetitiveness and the stability are high.

The invention discloses a sample bag. The sample bag comprises a first sealing strip, a second sealing strip, a first metal wire and a second metal wire. A bag mouth can be conveniently closed or opened by utilizing the components, and the sample bag has the advantages that the sampling is easy, and the storage and carrying are convenient. Pulling lugs are respectively and fixedly arranged at the central positions of the second sealing strip and the first sealing strip, and parts of the pulling lugs are bonded and fixed between the sealing strips and plastic films. As the parts of the pulling lugs can be machined together with the sealing strips and the plastic films, and clung to and fixed with one another, the machining can be completed in one step, and the production cost is further reduced. The sample bag solves the ubiquitous problems of complication in operation and inconvenience in carrying in the prior art, and the production cost is lowered.

The invention discloses a backpressure equal-channel angular extruding mould which comprises a pressing plate, a guide pillar, a knocking bar, a convex mould, a guide sleeve, a spring, a concave mould, a mould base, a double-fork type punching head, a heating ring, a positioning slide block, a backpressure ejecting rod and an ejecting rod bracket, wherein the rigidity and strength of punching head are increased by the double-fork type punching head; the positioning slide block capable of sliding back and forth is arranged in a horizontal channel, so that the last sample can be conveniently taken out from the horizontal channel; and backpressure is applied to the sample by a backpressure device in an ejecting rod pressurizing form during an extruding process, so that the extruding temperature is efficiently reduced, the grain is refined and the comprehensive mechanical property of magnesium alloy is improved.

The invention relates to a fluorescence labeling tracer and a preparation method thereof. The fluorescence labeling tracer is a product which is obtained by reacting a polymer of acrylic acid-hydroxy-propyl acrylate copolymer or acrylic acid-acrylamide copolymer and an organic fluorescence derivating agent of L-tryptophan, tyrosine, sulfanilic acid or sodium sulfanilate. The fluorescence labeling tracer is high in sensitivity and selectivity. When used for detecting the concentration of a water treatment agent in an industrial water system, the fluorescence labeling tracer is quick and convenient, and high in repeatability, samples can be taken easily, the required amount of samples is small, and the fluorescence labeling tracer can be used for directly and continuously detecting the running condition of a circulating water system.

The present invention relates to a biological sample collection kit, which uses a unique dry preservative for the preservation of nucleic acids, DNA and RNA, for collection, stabilization, transportation and long-term room temperature storage of biological samples that can be from sources such as saliva for molecular diagnostic applications. The invention features a collection device for biological samples such as saliva containing nucleic acids, and dry preservatives that are affixed directly or to a carrier substrate and placed within the collection device such that the samples come into contact with the dry preservatives resulting in the preservation and stabilization of the nucleic acids.

The invention provides a method for analyzing tiny particles in drinking water. The method for analyzing the tiny particles in the drinking water comprises the following steps of A: dyeing a drinkingwater sample by using Nile red, then performing filtering, and collecting the tiny particles on filter paper; and B, detecting and analyzing chemical components of the tiny particles by adopting an FTIR infrared spectrometer, a scanning electron microscope and an X-ray spectrometer. According to the analysis method, the chemical components of the tiny particles in the water can be identified; thecontent of the chemical components can be qualitatively or semi-quantitatively analyzed; an analysis result is accurate; and sampling is easy.