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Application of Survivin antibody and esophageal cancer immunochromatography detecting test strip prepared therewith

A technology for immunochromatographic detection and esophageal cancer, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems that are not suitable for general population promotion, cannot achieve rapid screening, and is not conducive to popularization and application, and achieve fast detection speed, The effect of fast diagnosis and low cost

Inactive Publication Date: 2009-09-23
BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the above detection of esophageal cancer, the detection operation of each method requires professional technicians to carry out in a professional laboratory, the technical requirements are high, and the equipment is relatively expensive, and the time required for a single sample processing is long, and rapid screening cannot be achieved. In addition, the above-mentioned common detection methods for esophageal cancer include immunohistochemistry and radioimmunoassay based on molecular biological markers. When sampling, patients or high-risk groups need to endure certain pains and are not easy to obey.
The above-mentioned detection methods for esophageal cancer are not suitable for popularization in the general population, and it is not conducive to the promotion and application in the vast rural and remote areas with high incidence and large number of patients in my country.

Method used

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  • Application of Survivin antibody and esophageal cancer immunochromatography detecting test strip prepared therewith
  • Application of Survivin antibody and esophageal cancer immunochromatography detecting test strip prepared therewith
  • Application of Survivin antibody and esophageal cancer immunochromatography detecting test strip prepared therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of Survivin colloidal gold immunochromatographic detection test strip is provided in this embodiment, please refer to figure 1 As shown, the test strip of the present embodiment is provided with a cardboard base plate 5, and on the base plate 5, a blood-absorbing glass fiber membrane layer 4 (as a sample loading area) is arranged successively from left to right, and an anti-human Survivin polyclonal antibody is fixed. Glass fiber membrane layer 3, nitrocellulose membrane 2 and absorbent filter paper 1. Each layer is successively pasted on the cardboard bottom plate 5 alternately and assembled to form the Survivin colloidal gold immunochromatographic detection test strip of this embodiment.

[0038] Wherein, the size of the nitrocellulose membrane 2 is: length 10 cm, width 2.5 cm. On nitrocellulose membrane 2, be provided with T test area and C quality control area, described test area T is the zone band that is marked with anti-human Survivin monoclonal antibody...

Embodiment 2

[0056] Take 100 μl of serum from 2 pathologically diagnosed patients with esophageal cancer from the Laboratory Department of Beijing Cancer Hospital, and take 100 μl of serum from another 2 healthy patients as a control. Immediately after taking blood, use the Survivin colloidal gold immunochromatographic test paper of Example 1. strips are tested. Among them, in the detection of the serum of 2 patients with esophageal cancer, after inserting the bottom end of the test strip (glass fiber membrane layer 4 for absorbing serum) into the serum to be tested in the EP tube, the quality control line and the detection line were separated in about 1.5 minutes. Purple-red bands appeared everywhere; and in the detection of 2 cases of healthy human serum, after inserting the bottom end of the test strip (serum-absorbing glass fiber membrane layer 4) into the serum to be tested in the EP tube, about 1.5 A purplish red band appeared at the quality control line, but there was still no react...

Embodiment 3

[0058] In the Survivin colloidal gold immunochromatographic detection test strip of the present embodiment, the test area T is a zone marked with anti-human Survivin monoclonal antibody, and the concentration of Survivin monoclonal antibody is 0.6mg / ml; the quality control area C is the zone marked with goat anti-rabbit secondary antibody IgG, the concentration of the secondary antibody is 2 mg / ml; in the colloidal gold-labeled anti-human Survivin polyclonal antibody immobilized on the glass fiber membrane layer 3, the labeling concentration of the antibody is 15 μg / ml, The diameter of the colloidal gold particles is 20nm, and the OD280 value is adjusted to 0.8 when the colloidal gold is applied after labeling, and the application concentration is 50 μl / cm. Other parameters, structure and preparation method of the test strip are the same as in Example 1.

[0059] Using the Survivin colloidal gold immunochromatographic detection test strip of this example, 100 μl each of the se...

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Abstract

The invention provides an application of Survivin antibody, and particularly relates to an application for preparing an esophageal cancer immunochromatography detecting test strip. The invention also provides an esophageal cancer immunochromatography detecting test strip, comprising a nitric acid fibrin film layer and a glass fiber film layer, wherein, a T test area and a C quality control area are arranged on the nitric acid fibrin film layer, the test area comprises Survivin monoclonal antibody or polyclonal antibody, the quality control area is enveloped with second antibody IgG; the glassfiber film layer is fixed with the Survivin polyclonal antibody or monoclonal antibody which are marked by colloidal gold. The method simultaneously provides a method for detecting the esophageal cancer immunochromatography with the test strip. The method for detecting the esophageal cancer immunochromatography with the test strip which is invented with the monoclonal antibody or the polyclonal antibody has the advantages of non-invasive, simple operation, sensitivity, easy sampling and the like, can fast, directly and qualitatively detect the Survivin in a sample, and can be used for screening esophageal cancers for people in a high incidence area.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to the application of Survivin (survivin) antibody, specifically, the application of Survivin antibody in the preparation of esophageal cancer immunochromatographic detection test strips and the prepared esophageal cancer immunochromatographic detection test note. Background technique [0002] Esophageal cancer is a malignant tumor that occurs in the epithelial tissue of the esophagus. The occurrence of esophageal cancer is related to the chronic stimulation of nitrosamine compounds, inflammation and trauma, genetic factors, and the content of trace elements in drinking water, food and vegetables. The incidence of esophageal cancer in my country is very high, second only to gastric cancer. Esophageal cancer is the most common tumor in rural my country. It has been confirmed that the surgical resection rate of early detection of esophageal cancer can reach 100%, and the 5-year sur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577G01N33/558G01N33/552
Inventor 张青云
Owner BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL
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