The invention provides a goldmag particle-based acridinium ester
chemiluminescence immunological detection method of human
epididymis secretory protein (HE4). The goldmag particle-based acridinium ester
chemiluminescence immunological detection method mainly comprises following steps: (1) goldmag particle is taken as an immunoreaction and
solid-phase separation carrier, and HE4 coated
antibody is connected with the surface of the goldmag particle via
coupling; (2) blank sites on the surface of the goldmag particle, which are not combined with the HE4 coated
antibody, are blocked with a blocking solution; (3) acridinium ester (AE) is used for marking HE4 labelled
antibody; (4) a sample to be detected, and the acridinium ester marked HE4 antibody are added into the blocked HE4 antibody coated goldmag particle for reaction so as to obtain a double-antibody
sandwich compound, wherein the acridinium ester marked HE4 antibody is capable of realizing specific binding with HE4
antigen; (5) washing is carried out; and (6)
chemiluminescence detection is carried out. The goldmag particle-based acridinium ester chemiluminescence immunological detection method is high in detection sensitivity, specificity, accuracy, and stability, and is simple and rapid;
linearity range is wide; no
radioactive contamination is caused; and operation is safe.